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  4. Recombinant Human Prestin (SLC26A5), partial

Recombinant Human Prestin (SLC26A5), partial

  • 中文名称：
    人SLC26A5重组蛋白
  • 货号：
    CSB-YP021528HU
  • 规格：
    ￥1500
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    SLC26A5
  • Uniprot No.：
  • 别名：
    SLC26A5; PRESPrestin; Solute carrier family 26 member 5
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Cytoplasmic Domain
  • 来源：
    Yeast
  • 分子量：
    29.0kDa
  • 蛋白标签：
    N-terminal His-tagged/Tag-Free
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site.
  • 基因功能参考文献：
    1. Our study thus provides experimental evidence that supports a causal relationship between the R130S mutation in the prestin gene and hearing loss found in patients with this missense mutation. PMID: 27041369
    2. Prestin expression imparts susceptibility to 2-hydroxypropyl-beta-cyclodextrin-induced hearing loss. PMID: 26903308
    3. I hypothesize that serum assays of OHC specific protein, prestin, will allow detection and quantification of OHC damage before audiometric testing can identify presence of hearing loss. PMID: 25920562
    4. anion-pi interaction is the mechanism for the voltage-dependent response of prestin PMID: 26283790
    5. The findings suggest that CASK and the truncated prestin splice isoform contribute to confinement of prestin to the basolateral region of the plasma membrane. PMID: 23542924
    6. The effects of fast temperature jumps induced by an infrared (IR) laser in control and prestin (SLC26a5)-transfected human embryonic kidney (HEK) cells, are reproted. PMID: 24138858
    7. Calmodulin-prestin interaction may be involved in the medial olivocochlear-mediated modulation of cochlear amplification PMID: 24453323
    8. COCH and SLC26A5 mRNA are expressed in specific structures and cells of the inner ear in archival human temporal bone PMID: 23660400
    9. prestin subunits are individually functional within a given multimer PMID: 23212912
    10. This result implies that in cell membranes prestin oligomerizes to a tetramer. PMID: 21975444
    11. Four mutations (C124A, C192A, C260A, and C415A), all in nonconserved cysteinyl residues, significantly differed in their nonlinear capacitance properties compared with wild-type prestin. PMID: 21813750
    12. Observational study of genetic testing. (HuGE Navigator) PMID: 20668687
    13. These data reveal that the STAS (sulfate transporters and anti-sigma factor antagonist) domain starts immediately after the last transmembrane segment and lies beneath the lipid bilayer. PMID: 20471983
    14. Cysteine mutagenesis reveals transmembrane residues associated with charge translocation in prestin PMID: 19926791
    15. an essential function of prestin in human auditory processing PMID: 12719379
    16. KCNQ4 phosphorylation via PKA and coupling to a complex that may include prestin can lead to the negative activation and the negative resting potential found in adult outer hair cells. PMID: 15660259
    17. Because mammals possess differentiated outer hair cells (OHC), they also benefit from a novel electromotile process, powered by the motor protein, prestin. PMID: 16873410
    18. Packing of helices and interactions between residues surrounding the sulfate transporter motif is essential for normal prestin activity. PMID: 17151276
    19. This is the first genetic and electrophysiological analysis of a human mutation in a coding exon of the pres gene by 47 patients with non-syndromic, sensorineural, mild-to-moderate hearing impairment. PMID: 17786286
    20. Cholesterol levels modulate the distribution of prestin within plasma membrane microdomains and affect prestin self-association PMID: 17933870
    21. results indicate that an evolutionarily conserved dimeric quaternary structure represents the native and functional state of SLC26 transporters PMID: 18073211
    22. Results quantify the relation between membrane cholesterol concentration and prestin-associated charge movement and enhance our understanding of how membrane composition modulates prestin function. PMID: 18567583
    23. heterozygosity for the mutation IVS2-2A>G in SLC26A5 gene may not, by itself, be sufficient to cause hearing loss. PMID: 19027966
    24. analysis of novel DNA sequence variations in SLC26A5, encoding prestin PMID: 19492055
    25. Results are consistent with significant movements in the COOH-terminal domain of prestin upon change in membrane potential, providing the first dynamic information on its molecular rearrangements. PMID: 19515900
    26. This indicates that Met-225 in prestin somehow adjusts nonlinear capacitance and the motility of prestin-expressing cells. PMID: 19737539
    27. Observational study of gene-disease association. (HuGE Navigator) PMID: 19492055
    28. Observational study of gene-disease association. (HuGE Navigator) PMID: 19027966
    29. Observational study of gene-disease association. (HuGE Navigator) PMID: 18988928
    30. Observational study of genotype prevalence. (HuGE Navigator) PMID: 16086836

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  • 相关疾病：
    Deafness, autosomal recessive, 61 (DFNB61)
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    SLC26A/SulP transporter (TC 2.A.53) family
  • 数据库链接：

    HGNC: 9359

    OMIM: 604943

    KEGG: hsa:375611

    STRING: 9606.ENSP00000304783

    UniGene: Hs.585146