Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Human Pre-B-cell leukemia transcription factor 1 (PBX1)

Recombinant Human Pre-B-cell leukemia transcription factor 1 (PBX1)

  • 中文名称：
    人PBX1重组蛋白
  • 货号：
    CSB-YP017505HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PBX1重组蛋白
  • 货号：
    CSB-EP017505HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PBX1重组蛋白
  • 货号：
    CSB-EP017505HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PBX1重组蛋白
  • 货号：
    CSB-BP017505HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PBX1重组蛋白
  • 货号：
    CSB-MP017505HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PBX1
  • Uniprot No.：
  • 别名：
    DKFZp686B09108; Homeo box protein PBX1; Homeo box protein PRL; Homeobox protein PBX 1; Homeobox protein PBX1; Homeobox protein PRL; MGC126627; PBX 1; Pbx1; PBX1_HUMAN; Pre B cell leukemia homeobox 1; Pre B cell leukemia transcription factor 1; Pre-B-cell leukemia transcription factor 1; PRL
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-430
  • 氨基酸序列
    MDEQPRLMHS HAGVGMAGHP GLSQHLQDGA GGTEGEGGRK QDIGDILQQI MTITDQSLDE AQARKHALNC HRMKPALFNV LCEIKEKTVL SIRGAQEEEP TDPQLMRLDN MLLAEGVAGP EKGGGSAAAA AAAAASGGAG SDNSVEHSDY RAKLSQIRQI YHTELEKYEQ ACNEFTTHVM NLLREQSRTR PISPKEIERM VSIIHRKFSS IQMQLKQSTC EAVMILRSRF LDARRKRRNF NKQATEILNE YFYSHLSNPY PSEEAKEELA KKCGITVSQV SNWFGNKRIR YKKNIGKFQE EANIYAAKTA VTATNVSAHG SQANSPSTPN SAGSSSSFNM SNSGDLFMSV QSLNGDSYQG AQVGANVQSQ VDTLRHVISQ TGGYSDGLAA SQMYSPQGIS ANGGWQDATT PSSVTSPTEG PGSVHSDTSN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor which binds the DNA sequence 5'-TGATTGAT-3' as part of a heterodimer with HOX proteins such as HOXA1, HOXA5, HOXB7 and HOXB8. Binds to the DNA sequence 5'-TGATTGAC-3' in complex with a nuclear factor which is not a class I HOX protein. Has also been shown to bind the DNA sequence 5'-ATCAATCAA-3' cooperatively with HOXA5, HOXB7, HOXB8, HOXC8 and HOXD4. Acts as a transcriptional activator of PF4 in complex with MEIS1. Also activates transcription of SOX3 in complex with MEIS1 by binding to the 5'-TGATTGAC-3' consensus sequence. In natural killer cells, binds to the NFIL3 promoter and acts as a transcriptional activator of NFIL3, promoting natural killer cell development. Plays a role in the cAMP-dependent regulation of CYP17A1 gene expression via its cAMP-regulatory sequence (CRS1). Probably in complex with MEIS2, involved in transcriptional regulation by KLF4. Acts as a transcriptional activator of NKX2-5 and a transcriptional repressor of CDKN2B. Together with NKX2-5, required for spleen development through a mechanism that involves CDKN2B repression.; As part of a PDX1:PBX1b:MEIS2B complex in pancreatic acinar cells, is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element.
  • 基因功能参考文献：
    1. PBX1 plays an oncogenic role in clear cell renal carcinoma via JAK2/STAT3 pathway PMID: 29678569
    2. B-cell acute lymphoblastic leukemia patients with positive E2A-PBX1 fusion expression after transplant will have a poor prognosis. PMID: 29705861
    3. Expression of PBX1 in leukemia cells enhances chemo-resistance and colony formation. PMID: 28034988
    4. The sequence variants in our cases included missense substitutions adjacent to the PBX1 homeodomain (p.Arg184Pro, p.Met224Lys, and p.Arg227Pro) or within the homeodomain (p.Arg234Pro, and p.Arg235Gln), whereas p.Ser262Glnfs*2, and p.Arg288* yielded truncated PBX1 proteins. PMID: 29036646
    5. PBX1 is one of the determinants in the Cx32 promoter targeting site, preventing further damage of gap junction protein in H. pylori-associated gastric carcinogenesis. PMID: 28839434
    6. A hydrophobic residue in the TALE homeodomain of PBX1 promotes epithelial-to-mesenchymal transition of gastric carcinoma PMID: 28514754
    7. PBX1 haploinsufficiency leads to syndromic CAKUT. PMID: 28270404
    8. This is the first time the protein partners of either E2A-PBX1 or HOXA9 oncoproteins were identified using an unbiased biochemical approach. The identification of translation initiation factors associated with HOXA9 might indicate a novel function for HOX proteins independent of their transcriptional activity. PMID: 28707666
    9. involved in monogenic anomalies of kidney and urinary tract PMID: 28566479
    10. these results confirm that PBX1 directly regulates genes related to T cell activation and shows that the lupus-associated isoform PBX1-d has unique molecular functions. PMID: 28257976
    11. the PBX1 binding motif at its promoter acted to positively regulate STAT3 transcription. PMID: 27590741
    12. Our results suggest that Pbx1-d impacts lupus development by regulating effector T cell differentiation and promoting follicular helper T cells at the expense of regulatory T cell s and identify Pbx1 as a novel regulator of CD4(+) T cell effector function PMID: 27296664
    13. Notably, PBX1 and NFE2L1 levels are severely reduced in dopaminergic neurons of the substantia nigra of Parkinson's disease (PD) patients and decreased NFE2L1 levels increases damage by oxidative stress in human midbrain cells. PMID: 27354364
    14. PBX1 overexpression in MCF10A cells up-regulated most LiMe genes. PMID: 28263391
    15. results indicate that E2A-PBX1 cooperates with additional gene alterations to develop acute lymphoblastic leukemia; among them, enhanced expression of ZNF521 may play a clinically relevant role in E2A fusion genes to develop B-lineage ALL PMID: 27088431
    16. Review of the role of the E2A-PBX1 gene rearrangement in the prognosis of childhood acute lymphoblastic leukemia and its central nervous system relapse. PMID: 26509298
    17. This study thus suggests that RNF6 overexpression in leukemia is under the direction of PBX1 and that the PBX1/RNF6 axis can be developed as a novel therapeutic target of leukemia. PMID: 26971355
    18. Data identifies PBX1 amplification as a functional hallmark of aggressive ERalpha-positive breast cancers. PMID: 26215677
    19. We observed significant enrichment of the neuroactive ligand-receptor interaction pathway in TCF3-PBX1 as well as an enrichment of genes involved in immunity and infection pathways in ETV6-RUNX1 subtype PMID: 26237075
    20. This association study successfully identified two susceptibility Single Nucleotide Polymorphisms, (WNT9B and PBX1) associated with Mayer-Rokitansky-Kuster-Hauser syndrome risk, both separately and interactively. PMID: 26075712
    21. PBX1 promotes frontal cortex patterning by repressing genes that promote dorsocaudal fate. PMID: 26671461
    22. Hematopoietic pre-B cell leukemia transcription factor interacting protein enhances gastric cancer cell migration and invasion, and modulates epithelial-mesenchymal transition, which plays a key role in cancer cell migration and invasion. PMID: 26211905
    23. When intensive chemotherapy was used, the TCF3-PBX1 was associated with a favorable outcome in childhood pre-B ALL. PMID: 25551271
    24. Induction of PBX1 expression was associated with 13-cisRA responsiveness in neuroblastoma. PMID: 24947929
    25. In this study, we showed that TCF3-PBX1 positive pediatric BCP-ALL patients treated according to the JACLS ALL02 and CCLSG ALL2004 protocol had favorable outcomes PMID: 24578304
    26. E2A-PBX1 fusion gene caused by t(1;19)(q23;p13) may be a common genetic change in AIS and a survival determinant for female AIS patients at early stage. PMID: 23688269
    27. Data indicate that among 31 thymus development-related genes, PBX1 copy number gain and FOXC1 copy number loss were presented in 43.0% and 39.5% of the tumors, respectively. PMID: 23444221
    28. E2a-pbx1-positive patients are associated with more aggressive acute lymphoblastic leukemia. PMID: 23511488
    29. The results demonstrate that MEOX1 is a critical target gene and cofactor of PBX1 in ovarian cancers. PMID: 22567123
    30. PBX1 is a novel pioneer factor defining aggressive ERalpha-positive breast tumors, as it guides ERalpha genomic activity to unique genomic regions promoting a transcriptional program favorable to breast cancer progression. PMID: 22125492
    31. Splice isoform PBX1-d is expressed more frequently in CD4+ T cells from lupus patients than from healthy controls. Its presence correlates with an increased central memory T cell population. PMID: 22180614
    32. vthe importance of the Hox-Pbx interaction for the oncogenic activity of Hoxa1 PMID: 21957483
    33. Klf4 recruits a complex of Meis and Pbx proteins to DNA, resulting in Meis2 transcriptional activation domain-dependent activation of a subset of Klf4 target genes. PMID: 21746878
    34. Identified 2 known single nucleotide polymorphisms, which indicates that mutations in the coding sequence of PBX1 are not responsible for Mullerian duct abnormalities in Chinese women. PMID: 21575942
    35. The C-terminal extension of PBX1 folds to form a fourth alpha-helix to a level of 5-10%, even in the absence of binding partners. This suggests that PBX1 transiently preorganizes prior to binding DNA. PMID: 21087615
    36. Data show that binding of DHS with sequences of HNF1, CDX2, and PBX1 in vitro contribute to understanding of the complexity of cell-type-specific CFTR regulatory mechanisms. PMID: 19782160
    37. Data demonstrated that overexpressed PBX1 and MEIS1 increased endogenous SOX3 protein expression in both uninduced and RA-induced NT2/D1 cells. PMID: 19799567
    38. these studies demonstrate that the homeodomain proteins, MEIS1, PBX1B, and PBX2, play an important role in megakaryocytic gene expression PMID: 12609849
    39. PBX1 complexed with HOXA9 and DNA, so that the posterior Hox hexapeptide adopts an altered conformation. PMID: 12923056
    40. E2a-Pbx1 and Bmi-1 are likely to play a role in the pathogenesis of human lymphoid leukemias through downregulation of the INK4A-ARF gene PMID: 14536079
    41. E2A-PBX1 interacts directly with the KIX domain of CBP/p300 in the induction of proliferation in primary hematopoietic cells PMID: 15507449
    42. HoxD9 and Pbx1 are inappropriately expressed in most human esophageal squamous cell carcinoma. Understanding the role of Hox genes in esophageal epithelial cell carcinogenesis may not only augment early detection but also offer possiblde treatment. PMID: 15770739
    43. In pancreatic exocrine tissue from obese non-diabetic subjects with increased islet mass, we found that Pbx-1 and Pdx-1 were up-regulated (5.9+/-1.2 and 2.4+/-0.6 versus non-obese). PMID: 15979049
    44. No coding variant was associated with diabetes and no association was found among African American subjects. Three variants in Caucasians were associated with T2DM. Three variants were significant determinants of insulin sensitivity PMID: 16140554
    45. The patients with immunophenotype of Pre-B-acute lymphoblastic leukemia were found to carry: E2A/PBX1 and E2A/HLF. PMID: 16215946
    46. Required for pro-angiogenic homeobox Hox DNA binding and transcriptional activity in endothelial cells. PMID: 16328158
    47. Androgen-independent cell line DU145 cells lack PLZF gene expression, resulting in the upregulation of Pbx1 and HoxC8 expression. The Pbx1-HoxC8 heterocomplex may lead to androgen-independent growth in prostate cancer. PMID: 16637071
    48. Targeted-E2A-PBX1 inhibition leads to reduced expression of the EB-1 and Wnt16b genes; aberrant expression of these genes may be a key step in leukemogenesis in t(1;19)-positive pre-B leukemia. PMID: 16769578
    49. Amino acid substitutions that prevent helix formation prevent both the interaction with KIX domain of cyclic AMP response element-binding protein (CBP) and cell immortalization by E2A-PBX1. PMID: 16914730
    50. Expanded polyhistidine repeats in HOXA1 enhance aggregation and cell death, resulting in impaired neuronal differentiation and cooperative binding with PBX1. PMID: 17131398

    显示更多

    收起更多

  • 相关疾病：
    Congenital anomalies of kidney and urinary tract syndrome with or without hearing loss, abnormal ears, or developmental delay (CAKUTHED)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TALE/PBX homeobox family
  • 组织特异性：
    Expressed in the kidney. Expressed in the endothelial cells of the glomeruli and interstitium (at protein level). Expressed in all tissues except in cells of the B and T lineage. Expressed strongly in kidney and brain.
  • 数据库链接：

    HGNC: 8632

    OMIM: 176310

    KEGG: hsa:5087

    STRING: 9606.ENSP00000405890

    UniGene: Hs.557097