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  4. Recombinant Human Polycomb group RING finger protein 2 (PCGF2)

Recombinant Human Polycomb group RING finger protein 2 (PCGF2)

  • 中文名称：
    人PCGF2重组蛋白
  • 货号：
    CSB-YP017606HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PCGF2重组蛋白
  • 货号：
    CSB-EP017606HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PCGF2重组蛋白
  • 货号：
    CSB-EP017606HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PCGF2重组蛋白
  • 货号：
    CSB-BP017606HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PCGF2重组蛋白
  • 货号：
    CSB-MP017606HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DNA binding protein Mel 18; DNA-binding protein Mel-18; Mel 18; MGC10545; PCGF 2; PCGF2; PCGF2_HUMAN; Polycomb group ring finger 2; Polycomb group RING finger protein 2; RING finger protein 110; RNF110; Zinc finger protein 144; ZNF 144; ZNF144
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-344
  • 氨基酸序列
    MHRTTRIKIT ELNPHLMCAL CGGYFIDATT IVECLHSFCK TCIVRYLETN KYCPMCDVQV HKTRPLLSIR SDKTLQDIVY KLVPGLFKDE MKRRRDFYAA YPLTEVPNGS NEDRGEVLEQ EKGALSDDEI VSLSIEFYEG ARDRDEKKGP LENGDGDKEK TGVRFLRCPA AMTVMHLAKF LRNKMDVPSK YKVEVLYEDE PLKEYYTLMD IAYIYPWRRN GPLPLKYRVQ PACKRLTLAT VPTPSEGTNT SGASECESVS DKAPSPATLP ATSSSLPSPA TPSHGSPSSH GPPATHPTSP TPPSTASGAT TAANGGSLNC LQTPSSTSRG RKMTVNGAPV PPLT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity.
  • 基因功能参考文献：
    1. Low expression of Mel-18 is correlated with gastric cancer. PMID: 27542229
    2. Mel-18 underexpression in luminal breast cancer cells caused ER-alpha downregulation.Its overexpression restored it in triple-negative breast cancer cells. MEL-18 suppressed SUMOylation of the ESR1 transactivators p53 and SP1. PMID: 25822021
    3. It was therefore concluded that the lower Mel-18 expression might contribute to colorectal cancer development/progression. PMID: 24964959
    4. Mel-18 functions as a tumor suppressor by its novel negative control of the epithelial-mesenchymal transition in breast cancer. PMID: 23474752
    5. PCGF2, a PRC1 gene, played a negative role in the granulocytic differentiation of human APL cells. PMID: 22085718
    6. these findings provide that Mel-18 is a novel regulator of tumor angiogenesis through regulating HIF-1alpha and its target VEGF expressions mediated by the PTEN/PI3K/Akt pathway, suggesting a new tumor-suppressive role of Mel-18 in human breast cancer. PMID: 21602890
    7. Loss of Mel-18 is associated with prostate cancer. PMID: 19395284
    8. Our analysis showed correlation between BMI1 and PCGF2 gene's expression and survival in children with medulloblastoma. PMID: 20717685
    9. Bmi-1/Mel-18 ratio can be potentially used as a tool for stratifying women at risk of developing breast malignancy. PMID: 21162745
    10. Mel-18 may serve as a useful marker in prognostic evaluation for patients with breast cancer. PMID: 20444850
    11. Decreased Mel-18 and increased Bmi-1 mRNA expression was associated with the carcinogenesis and progression of gastric cancer PMID: 21059209
    12. An association of Mel18 with emerin was observed in Hutchinson-Gilford progeria syndrome, but not in WT cells. PMID: 19727227
    13. Mel-18 plays a significant role in the angiogenic function of endothelial cells by regulating endothelial gene expression. PMID: 20801102
    14. BMI1 acts as an oncogene and Mel-18 functions as a tumor suppressor via downregulation of BMI1. PMID: 20170541
    15. The oncogenic role of MEL-18 in human primary breast carcinomas is determined by its capacity to inhibit INK4a/ARF proteins(p16INK4a, p14ARF, or h-TERT) or to induce telomerase activity. PMID: 17145810
    16. Our data suggest that Mel-18 regulates Bmi-1 expression during senescence via down-regulation of c-Myc. PMID: 17151361
    17. These results suggest that Bmi-1 and Mel-18 may have overlapping functions in cancer cell growth. PMID: 17452456
    18. Mel-18 and Bmi-1 may regulate the Akt pathway in breast cancer cells, and that Mel-18 functions as a tumor suppressor by repressing the expression of Bmi-1 and consequently down-regulating Akt activity. PMID: 17545584
    19. Results show that a phosphorylated form of Mel-18 targets the Ring1B histone H2A ubiquitin ligase to chromatin. PMID: 17936708
    20. The results of this study strengthen the conclusion that mel-18 functions as an anti-SUMO E3 factor, and extend its targets to include regulation of the sumoylation of the important cellular protein RanGAP1. PMID: 18706886
    21. Single Nucleotide Polymorphism and down regulation of Mel-18 is associated with prostate cancer. PMID: 19585577
    22. validated occurrence of an unusual TG 3' splice site in intron 1 PMID: 17672918
    23. The mouse counterpart of this gene regulates the expression of Th2 cytokines, and plays a critical role in Th2 cell differentiation and Th-2 dependent immune responses. PMID: 11520462
    24. The mouse counterpart of this gene regulates the expression of various chemokines and chemokine receptors, which may be important for T cell migration and differentiation. PMID: 11750047

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Detected in all tissues examined with high expression found in placenta lung and kidney and low expression, in liver, pancreas and skeletal muscle.
  • 数据库链接：

    HGNC: 12929

    OMIM: 600346

    KEGG: hsa:7703

    STRING: 9606.ENSP00000354033

    UniGene: Hs.371617