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  4. Recombinant Human ADP-ribose glycohydrolase ARH3 (ADPRHL2)

Recombinant Human ADP-ribose glycohydrolase ARH3 (ADPRHL2)

  • 中文名称：
    Recombinant Human ADP-ribose glycohydrolase ARH3(ADPRHL2),Yeast
  • 货号：
    CSB-YP868344HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human ADP-ribose glycohydrolase ARH3(ADPRHL2),Yeast
  • 货号：
    CSB-EP868344HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human ADP-ribose glycohydrolase ARH3(ADPRHL2),Yeast
  • 货号：
    CSB-EP868344HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human ADP-ribose glycohydrolase ARH3(ADPRHL2),Yeast
  • 货号：
    CSB-BP868344HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human ADP-ribose glycohydrolase ARH3(ADPRHL2),Yeast
  • 货号：
    CSB-MP868344HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ADPRHL2
  • Uniprot No.：
  • 别名：
    [Protein ADP ribosylarginine] hydrolase like protein 2; [Protein ADP-ribosylarginine] hydrolase-like protein 2; ADP ribosylhydrolase 3; ADP ribosylhydrolase like 2; ADP-ribosylhydrolase 3; Adprhl2; AI836109; ARH3; ARHL2_HUMAN; ecto ADP ribosylhydrolase; FLJ20446; OTTMUSP00000009757; Poly(ADP ribose) glycohydrolase ARH3; Poly(ADP-ribose) glycohydrolase ARH3; Protein ADP ribosylarginine hydrolase like protein 2; RP23-113P22.3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 表达区域：
    1-363
  • 氨基酸序列
    MAAAAMAAAAGGGAGAARSLSRFRGCLAGALLGDCVGSFYEAHDTVDLTSVLRHVQSLEPDPGTPGSERTEALYYTDDTAMARALVQSLLAKEAFDEVDMAHRFAQEYKKDPDRGYGAGVVTVFKKLLNPKCRDVFEPARAQFNGKGSYGNGGAMRVAGISLAYSSVQDVQKFARLSAQLTHASSLGYNGAILQALAVHLALQGESSSEHFLKQLLGHMEDLEGDAQSVLDARELGMEERPYSSRLKKIGELLDQASVTREEVVSELGNGIAAFESVPTAIYCFLRCMEPDPEIPSAFNSLQRTLIYSISLGGDTDTIATMAGAIAGAYYGMDQVPESWQQSCEGYEETDILAQSLHRVFQKS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    ADP-ribose glycohydrolase that preferentially hydrolyzes the scissile alpha-O-linkage attached to the anomeric C1'' position of ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on serine, free poly(ADP-ribose) and O-acetyl-ADP-D-ribose. Specifically acts as a serine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to serine residues on proteins, thereby playing a key role in DNA damage response. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Does not hydrolyze ADP-ribosyl-arginine, -cysteine, -diphthamide, or -asparagine bonds. Also able to degrade protein free poly(ADP-ribose), which is synthesized in response to DNA damage: free poly(ADP-ribose) acts as a potent cell death signal and its degradation by ADPRHL2 protects cells from poly(ADP-ribose)-dependent cell death, a process named parthanatos. Also hydrolyzes free poly(ADP-ribose) in mitochondria. Specifically digests O-acetyl-ADP-D-ribose, a product of deacetylation reactions catalyzed by sirtuins. Specifically degrades 1''-O-acetyl-ADP-D-ribose isomer, rather than 2''-O-acetyl-ADP-D-ribose or 3''-O-acetyl-ADP-D-ribose isomers.
  • 基因功能参考文献：
    1. Study results establish ARH3 as a serine mono-ADP-ribosylhydrolase and as an important regulator of the basal and stress-induced ADP-ribosylome. PMID: 29234005
    2. By screening for the hydrolase that is responsible for the reversal of Ser-ADP ribosilation, the s identified ARH3/ADPRHL2 as capable of efficiently and specifically removing Ser-ADP ribosilation of histones and other proteins. PMID: 28650317
    3. ADP-ribosylhydrolase 3 (ARH3), not poly(ADP-ribose) glycohydrolase (PARG) isoforms, is responsible for degradation of mitochondrial matrix-associated poly(ADP-ribose). PMID: 22433848
    4. ARH3 has poly(ADP-ribose) glycohydrolase activity but is structurally unrelated to poly(ADP-ribose) glycohydrolase PMID: 16278211
    5. cloning, recombinant production, purification and crystallization of ARH3 consisting of 347 amino-acid residues PMID: 16511307
    6. The molecular architecture of human ARH3 constitutes the archetype of an all-alpha-helical protein fold and suggests reversibility mechanisms of protein ADP-ribosylation. PMID: 17015823
    7. poly(ADP-ribose) glycohydrolase ARH3 hydrolyzed O-acetyl-ADP-ribose to produce ADP-ribose in a time- and Mg(2+)-dependent reaction and thus could participate in two signaling pathways PMID: 17075046

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Chromosome. Mitochondrion matrix.
  • 蛋白家族：
    ADP-ribosylglycohydrolase family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 21304

    OMIM: 610624

    KEGG: hsa:54936

    STRING: 9606.ENSP00000362273

    UniGene: Hs.18021