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  4. Recombinant Human Phosphatidylinositol-glycan-specific phospholipase D (GPLD1), partial

Recombinant Human Phosphatidylinositol-glycan-specific phospholipase D (GPLD1), partial

  • 中文名称：
    人GPLD1重组蛋白
  • 货号：
    CSB-YP009721HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人GPLD1重组蛋白
  • 货号：
    CSB-EP009721HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人GPLD1重组蛋白
  • 货号：
    CSB-EP009721HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人GPLD1重组蛋白
  • 货号：
    CSB-BP009721HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人GPLD1重组蛋白
  • 货号：
    CSB-MP009721HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GPLD1
  • Uniprot No.：
  • 别名：
    Glycoprotein phospholipase D; Glycosyl-phosphatidylinositol-specific phospholipase D; GPI-PLD; GPI-specific phospholipase D; GPIPLD; GPIPLDM; GPLD1; MGC22590; PHLD_HUMAN; Phosphatidylinositol glycan specific phospholipase D; Phosphatidylinositol-glycan-specific phospholipase D; PI-G PLD; PIGPLD; PIGPLD1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
  • 基因功能参考文献：
    1. computational study suggests that although curcumin to some extent binds with Tp receptor, yet the inhibition of Arf6GDP to Arf6GTP conversion appeared to be an important mechanism by which curcumin inhibits U46619-induced increase in PLD activity in PASMCs. PMID: 28780751
    2. Study discovered novel and independent associations of prediabetes and related traits with MASP1, and some evidence for associations with THBS1, GPLD1 and ApoA-IV, suggesting a role for these proteins in the pathophysiology of type 2 diabetes. PMID: 27344311
    3. c-Myc influences GPI-AP signaling transcriptionally and posttranslational and represses GPI-AP anti-proliferative signaling in tumors PMID: 27232633
    4. Low aggressive MCF-7 breast cancer cells have low endogenous PLD enzymatic activity and cell invasion, concomitant with high expression of miR-203, -887, and -3619 and miR-182 and miR-182. PMID: 26567912
    5. suggest that the down-regulation of GPI-PLD protein may be involved in prion propagation in the brains of prion diseases PMID: 25867459
    6. our findings showed that ANRIL is an lncRNA responsible in anti-tumorigenesis caused by PLD inhibition and combined incorporation of ANRIL into PLD inhibition-induced anti-tumorigenic signaling network PMID: 25964559
    7. AMPK suppresses PLD activity, and PLD suppresses AMPK via mTOR. PMID: 25632961
    8. Functional regulation of phospholipase D expression in cancer and inflammation. PMID: 24990948
    9. At the cellular level, PLD and its reaction product, phosphatidate, interact with a large number of protein partners that are directly related to the actin cytoskeleton and cell migration. PMID: 24990954
    10. PLD1 and PLD2 mutants inhibit very-low-density lipoprotein-induced aldosterone production in HAC15 cells. PMID: 24956203
    11. This study indicated that PLD participates in the same signaling pathway in this breast cancer cell line. PMID: 22481092
    12. genetic variation of GPLD1 appears to associate with circulating glycosylphosphatidylinositol-specific phospholipase D levels PMID: 22260953
    13. The mRNA expression and activity of GPI-PLD in de novo and refractory or relapsed acute myeloid leukemia patients are obviously higher than those in normal controls. PMID: 20137110
    14. This study revealed the novel mechanism of bleomycin-induced redox-sensitive activation of phospholipase D leading to the generation of phosphaticid acid. PMID: 21131602
    15. The role of phospholipase D in the transport of VAT1 to plasma membranes and in phosphatidic acid metabolism in neutrophils is reported. PMID: 20858461
    16. In human ovarian cancer cell lines, GPI-PLD mRNA expression correlated with GPI-PLD enzyme activity and with the shedding of the GPI-anchored tumor and prognostic markers, urokinase receptor and CA125, from the cell surface. PMID: 12090469
    17. increased serum glycosylphosphatidylinositol-specific phospholipase D is associated with insulin resistance in a cohort with a wide range of insulin sensitivity PMID: 14767861
    18. identified as component of human trypanosome lytic factor 1 (TLF1) PMID: 15500911
    19. PLD activation is a novel finding for the D(3) receptor, and is the first example of an effector system where D(3) signals without G(i)/G(o) protein intermediates. PMID: 15500962
    20. CTLA-4 may be stored in a specialized compartment in regulatory T cells that can be triggered rapidly for deployment to the plasma membrane in a phospholipase D- and ADP ribosylation factor-1-dependent manner PMID: 15814706
    21. Data describe the role of angiotensin II type 1A receptor phosphorylation, phospholipase D, and extracellular calcium in isoform-specific protein kinase C membrane translocation responses. PMID: 16831865
    22. GPI-PLD probably had no contribution to hematopoietic stem cell homing, which may due to its low or no expression in cord blood, bone marrow and mobilized peripheral blood CD34(+) cells. PMID: 17467053
    23. Data indicate that a critical component of Ras signaling is the activation of PLD. PMID: 17949898
    24. We show that human PLD1b and PLD2a contain functional caspase 3 cleavage sites and identify the critical aspartate residues within PLD1b that affect its activation by phorbol esters. PMID: 18298948
    25. The serum GPI-PLD activities, the protein and mRNA levels of GPI-PLD in hepatocellular carcinoma patients were decreased by 40%, 60% and 56%, respectively. PMID: 19135435

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    GPLD1 family
  • 数据库链接：

    HGNC: 4459

    OMIM: 602515

    KEGG: hsa:2822

    STRING: 9606.ENSP00000230036

    UniGene: Hs.533291