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  4. Recombinant Human PRKC apoptosis WT1 regulator protein (PAWR)

Recombinant Human PRKC apoptosis WT1 regulator protein (PAWR)

  • 货号：
    CSB-YP842688HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP842688HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP842688HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP842688HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP842688HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    2310001G03Rik; PAR 4; PAR-4; Pawr; PAWR_HUMAN; PRKC Apoptosis WT1 Regulator; PRKC apoptosis WT1 regulator protein; Prostate apoptosis response 4 protein; Prostate apoptosis response protein 4; prostate apoptosis response protein PAR-4; Transcriptional repressor Par-4-like protein PAWR; Transcriptional repressor PAR4; WT1 Interacting Protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-340
  • 氨基酸序列
    MATGGYRTSS GLGGSTTDFL EEWKAKREKM RAKQNPPGPA PPGGGSSDAA GKPPAGALGT PAAAAANELN NNLPGGAPAA PAVPGPGGVN CAVGSAMLTR AAPGPRRSED EPPAASASAA PPPQRDEEEP DGVPEKGKSS GPSARKGKGQ IEKRKLREKR RSTGVVNIPA AECLDEYEDD EAGQKERKRE DAITQQNTIQ NEAVNLLDPG SSYLLQEPPR TVSGRYKSTT SVSEEDVSSR YSRTDRSGFP RYNRDANVSG TLVSSSTLEK KIEDLEKEVV RERQENLRLV RLMQDKEEMI GKLKEEIDLL NRDLDDIEDE NEQLKQENKT LLKVVGQLTR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.
  • 基因功能参考文献：
    1. These findings suggest that PAR4 plays a potential tumor suppressor role in esophageal squamous cell carcinoma cells PMID: 30363984
    2. we determined that increased miR-17-3P level plays crucial role in CRC cells survival by targeting Par4, contributing to colorectal carcinogenesis. PMID: 29115593
    3. PAR4 is the target of mir-107 in colorectal cancer cells. PMID: 27938501
    4. we confirmed that the RASSF2-PAR-4 axis was mainly responsible for miR-7 functions in CAFs using bioinformatics methods. Overexpression of miR-7 in CAFs led to down-regulation of RASSF2, which dramatically decreased the secretion of PAR-4 from CAFs and then enhanced the proliferation and migration of the co-cultured cancer cells. PMID: 27901488
    5. we investigated in the present study the mechanisms regulating the accumulation of a 25kDa cleaved-Par-4 (cl-Par-4) fragment in ovarian and endometrial cancer cell lines PMID: 27175591
    6. s demonstrate that TRIM21 expression predicts survival in pancreatic cancer patients. This work highlights a novel mechanism of Par-4 regulation, and identifies a novel prognostic marker and potential therapeutic target for pancreatic cancer. PMID: 27830973
    7. Data suggest that PAR4 and P2Y12 heterodimer internalization/endocytosis is required for beta-arrestin-2 recruitment to endosomes and up-regulation of Akt signaling; activation of PAR4 but not of P2Y12 drives internalization of the PAR4-P2Y12 heterodimer. (PAR4 = protease-activated receptor 4; P2Y12 = purinergic receptor P2Y, G-protein coupled, 12 protein; Akt = proto-oncogene protein c-akt) PMID: 28652403
    8. In this review, we will focus on the therapeutic perspective of Par-4 with a special reference to its (Par-4) virgin prospect of devastating metastasis control. PMID: 27568374
    9. in vitro and in vivo upregulation of Par-4 expression is indispensable for the trafficking of GRP78 to the cell membrane and subsequent apoptosis of cancer cell PMID: 28720068
    10. Prostate apoptosis response 4 (PAR4) expression modulates WNT signaling pathways in MCF7 breast cancer cells: A possible mechanism underlying PAR4-mediated docetaxel chemosensitivity. PMID: 28259909
    11. Decreased PAR4 expression in breast cancer is associated with shorter survival. PAR4 suppresses growth and invasiveness of breast cancer cells. PMID: 26977019
    12. s determined that PAR-4 induces cell apoptosis in response to stimuli, in vitro, but is also involved in the relocation of GRP78 from endoplasmic reticulum to the cell surface of ovarian cancer cell line. PMID: 26246468
    13. These results suggest that Porphyromonas gingivalis activates PAR4 signaling pathways, leading proMMP9 over-expression and cellular invasion in oral squamous cell carcinoma cells. PMID: 25670650
    14. PAR1-platelet releasate enhances vasculogenesis more potently than PAR4-platelet releasate, and the enhancements require a cooperation of multiple platelet-derived angiogenic regulators. PMID: 25495701
    15. A novel long non-coding RNA T-ALL-R-LncR1 knockdown and Par-4 cooperate to induce cellular apoptosis in T-cell acute lymphoblastic leukemia cells. PMID: 23906015
    16. Data indicate that PAR1 and PAR4 activate common promigratory signalling pathways in Hep3B liver carcinoma cells including activation of the receptor tyrosine kinases Met and PDGFR, the formation of ROS and the inactivation of PTP1B. PMID: 25373316
    17. a Par-4 mutant that is unable to bind Fbxo45 is stabilized and further enhances staurosporine-induced apoptosis. PMID: 24992930
    18. C-terminus of the rat homologue of Par-4 was crystallized and a 3.7 A resolution X-ray diffraction data set was collected PMID: 25195896
    19. These results indicate that the expression of PAR1 and PAR2 in esophageal squamous cell carcinoma is increased but PAR4 is decreased. PMID: 25297082
    20. our results indicate that the mechanism by which PAR-4 orchestrates the apoptotic process requires cleavage by caspase-8. PMID: 24931006
    21. Par-4 is expressed in trophoblastic cells and is involved in transport of GRP78 to the cell surface. PMID: 24282526
    22. The addition of TRAIL to WIN 55.212-2-treated cells led to apoptotic death probably mediated by up-regulation of the tumor suppressor factor PAR-4, whose levels increased after WIN treatment, and by the translocation of GRP78 on cell surface. PMID: 24795528
    23. The cancer cell specific activity of Par-4 is elicited through intracellular as well as extracellular mechanisms. PMID: 25001535
    24. prostate apoptosis response-4 (Par-4) has a role in human glioma stem cells in drug-induced apoptosis PMID: 24523904
    25. These results suggested a prognostic role of Par-4 in hypopharyngeal carcinoma. PMID: 24418097
    26. Phosphorylation by CK2 impairs Par-4 proapoptotic functions. PMID: 24457960
    27. Par-4 is a target of TGF-beta signaling and acts as an important factor during TGF-beta-induced epithelial-to-mesenchymal transition. PMID: 24503536
    28. Par-4 expression modulates apoptosis in response to docetaxel in MCF7 breast cancer cells. PMID: 23760770
    29. Down-regulation of protease-activated receptor 4 in lung adenocarcinoma is associated with a more aggressive phenotype PMID: 23886184
    30. Par-4-induced multinucleation as a mechanism of cell death in oncogene-addicted cells and establish Par-4 as a negative regulator of breast cancer recurrence. PMID: 23770012
    31. our results identify a novel intracellular pathway of apoptosis mediated by NF-kappaB through UACA elevation, which by attenuating endoplasmic reticulum stress and GRP78 translocation to the cell surface can blunt the sensitivity of cancer cells to apoptosis. PMID: 23204231
    32. Gamma-tocotrienol inhibited IL-13/STAT6-activated eotaxin secretion via up-regulation of PAR4 expression and enhancement of aPKC-PAR4 complex formation. PMID: 21764283
    33. a novel mechanism of apoptosis induction by PAR-4/ceramide-enriched exosomes, which may critically contribute to Alzheimer disease. PMID: 22532571
    34. identified a novel specific caspase-3 cleavage site in Par-4, and the cleaved fragment of Par-4 retains proapoptotic activity PMID: 22184067
    35. The biological significance of PrPc association with par-4 provided the first evidence of a relationship between the endogenous levels of PrPc and the resistance of glioma cells to the apoptotic effects of TMZ. PMID: 21328340
    36. 17beta-estradiol and Insulin-like growth factor-1 inhibit PAR-4 gene expression in MCF-7 cells. PMID: 21567071
    37. Par-4-overexpressing tumors exhibited a bystander effect on wild-type tumors growing distally in the same mouse. PMID: 21555373
    38. The expression of PAR-4 protein in B cells correlated positively with the percentage of CD38(+) cells, as well as with CD38(+)/ZAP-70(+) cells. PMID: 21526495
    39. Decreased Par-4 expression is associated with cholangiocarcinoma. PMID: 20724592
    40. siRNA against Par-4 gene could inhibit the apoptosis of human bone marrow mesenchymal stem cells. PMID: 19099901
    41. findings suggest that a lower expression level of Par-4 is related to an unfavorable prognosis in breast cancer patients PMID: 20637369
    42. Downregulation of PAR4 is associated with poor prognosis in breast cancer. PMID: 20514395
    43. Compared to normal controls, mean Par-4 levels appeared slightly lower in schizophrenia and bipolar disorder. However, in major depression, Par-4 was decreased by 67% compared to normal controls. PMID: 20067857
    44. Data show that RASSF2 forms a direct and endogenous complex with prostate apoptosis response protein 4 (PAR-4) and that this interaction is regulated by K-Ras and is essential for the full apoptotic effects of PAR-4. PMID: 20368356
    45. Endoplasmic reticulum stress causes translocation of the Par-4-GRP78 complex from the ER to the plasma membrane, and through a positive feedback loop, extracellular Par-4 binds to cell surface GRP78 activating the extrinsic apoptotic pathway. Review. PMID: 19823030
    46. Results suggest that, in breast cancer, Par-4 plays a similar tumor suppressor gene role as reported in endometrial carcinoma, and that Par-4 expression has a significant inverse association with expression of progesterone receptor. PMID: 20082875
    47. These data suggest PAWR is a novel PITX2-interacting protein that regulates PITX2 activity in ocular cells. PMID: 19801652
    48. mechanical strain increased PAR-4 gene expression in macrophages PMID: 11910304
    49. role in regulating Bcl-2 through a WT1-binding site on the bcl-2 promoter PMID: 12644474
    50. Par-4 enables cells to circumvent inhibition of the central executioner caspase-3 by alternative activation of caspases following a decrease in expression levels of inhibitors of apoptosis proteins PMID: 12685825

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 组织特异性：
    Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers.
  • 数据库链接：

    HGNC: 8614

    OMIM: 601936

    KEGG: hsa:5074

    STRING: 9606.ENSP00000328088

    UniGene: Hs.643130