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  4. Recombinant Human PIN2/TERF1-interacting telomerase inhibitor 1 (PINX1)

Recombinant Human PIN2/TERF1-interacting telomerase inhibitor 1 (PINX1)

  • 中文名称：
    人PINX1重组蛋白
  • 货号：
    CSB-YP850264HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PINX1重组蛋白
  • 货号：
    CSB-EP850264HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PINX1重组蛋白
  • 货号：
    CSB-EP850264HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PINX1重组蛋白
  • 货号：
    CSB-BP850264HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PINX1重组蛋白
  • 货号：
    CSB-MP850264HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PINX1
  • Uniprot No.：
  • 别名：
    67-11-3 protein; FLJ20565; Hepatocellular carcinoma-related putative tumor suppressor; Liver-related putative tumor suppressor; LPTL; LPTS; MGC8850; OTTHUMP00000224984; OTTHUMP00000224985; OTTHUMP00000224986; OTTHUMP00000225053; OTTHUMP00000225054; PIN2 interacting protein 1; Pin2-interacting protein X1; PIN2/TERF1-interacting telomerase inhibitor 1; PINX1; PINX1_HUMAN; Protein 67-11-3; TRF1-interacting protein 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-328
  • 氨基酸序列
    MSMLAERRRK QKWAVDPQNT AWSNDDSKFG QRMLEKMGWS KGKGLGAQEQ GATDHIKVQV KNNHLGLGAT INNEDNWIAH QDDFNQLLAE LNTCHGQETT DSSDKKEKKS FSLEEKSKIS KNRVHYMKFT KGKDLSSRSK TDLDCIFGKR QSKKTPEGDA SPSTPEENET TTTSAFTIQE YFAKRMAALK NKPQVPVPGS DISETQVERK RGKKRNKEAT GKDVESYLQP KAKRHTEGKP ERAEAQERVA KKKSAPAEEQ LRGPCWDQSS KASAQDAGDH VQPPEGRDFT LKPKKRRGKK KLQKPVEIAE DATLEETLVK KKKKKDSK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor.
  • 基因功能参考文献：
    1. Low PINX1 expression is associated with colorectal cancer cell migration, invasion and metastasis PMID: 30015978
    2. PinX1 silencing in CD133+ CSCs significantly decreased TRF1, Mad1, and p53 mRNA levels, while it increased hTERT and c-Myc mRNA levels. PMID: 30138944
    3. NF-kappaB p65 potentiated tumor growth via suppressing a novel target LPTS PMID: 29017500
    4. Our findings suggested that PinX1 could be a potential tumour suppressor in non-small-cell lung cancer and that loss of PinX1 promoted non-small-cell lung cancer progression. PMID: 28815183
    5. Low expression of PinX1 is associated with malignant behavior in basal-like breast cancer. PMID: 28586040
    6. The cost-effective expression of PinX1 could constitute a novel molecular predictor/marker for NSCLC management. PMID: 28372542
    7. PINX1 Polymorphisms are associated with Hepatocellular Carcinoma in Thai Patients with Chronic Hepatitis B Virus Infection. PMID: 27221889
    8. The stability of PinX1 is maintained in nucleolus in the presence of TERT. PMID: 26194824
    9. PinX1 negatively regulated ccRCC metastasis and the expression of MMP-2 and NF-kappaB-p65 PMID: 26033551
    10. High PinX1 expression significantly correlated with a worse 5-year overall and disease-specific survival in gliomas. PMID: 26261583
    11. Data show a novel molecular mechanism for PINX1 as an attenuator of estrogen receptor activity in breast cancer cell lines, furthering its role as a tumor suppressor gene in breast cancer. PMID: 26187699
    12. PinX1 protein expression is decreased in colorectal cancer patients and predicts a poor prognosis. PMID: 26211574
    13. PinX1 inhibits cell proliferation, migration and invasion in glioma cells. PMID: 25698538
    14. PinX1 expression was an independent negative prognostic factor for breast cancer patients. PMID: 25888829
    15. Two novel markers, rs7840785 (PINX1) and rs7844465 (ZHX2), are significantly associated with carotid intima-media thickness. PMID: 25746325
    16. These results confirmed the role of PinX1 as a major tumor suppressor gene in breast cancer cell lines. PMID: 24672800
    17. Data confirmed that levels of PinX1 and caspase 3, 8 and 9 expression were closely linked to the poor prognosis of colorectal cancer. PMID: 24839934
    18. the expression of PinX1 in CSCC patients is likely a predictor of the response to paclitaxel chemotherapy. PMID: 25045845
    19. The results suggest that activation of Prp43p by Gno1p/PINX1 within early pre-ribosomal particles is crucial for their subsequent maturation. PMID: 24823796
    20. Down-regulation of PinX1 play an important role in the tumorigenesis and development of urothelial carcinoma of the bladder. PMID: 24268029
    21. these findings suggested that PinX1 may maintain telomere integrity by regulating TRF1 stability and that hTERT may act as both a positive and a negative regulator of TRF1 homeostasis in a PinX1-dependent manner. PMID: 24415760
    22. Overexpression of PinX1 blocked Eca109 cell proliferation and induced cell apoptosis by downregulating telomerase activity. PMID: 23912465
    23. Data indicate that HPV16 E6 suppresses the expression of PinX1 via inhibiting p53 transcriptional activity, resulting in the enhancement of telomerase activity. PMID: 24412852
    24. Suggest that PinX1 could serve as a novel predictor for response to chemoradiotherapy in esophageal squamous cell carcinoma. PMID: 23341363
    25. Data are consistent with the earlier data that showed that PINX1 somatic mutation was rare in hepatocellular carcinomas, medulloblatomas, gastric carcinomas, and colorectal carcinomas. PMID: 22882268
    26. Silencing of PinX1 significantly reduced the localization of telomerase to telomere during mid-late S phase, suggesting the involvement of PinX1 in the cell cycle-dependent trafficking of hTERT to telomere. PMID: 22749911
    27. PinX1 may play important roles in NPC proliferation, migration and apoptosis and has application potential in tumor-targeted gene therapy PMID: 22316341
    28. one function of PinX1 is to stabilize TRF1 during mitosis, perhaps to promote transition into M phase of the cell cycle. PMID: 22331467
    29. Polo-like kinase 1 (Plk1) is a novel interacting protein of PinX1 and may negatively regulate the stability of PinX1 by mitotic phosphorylation. PMID: 20573420
    30. the telomerase inhibitor PinX1 is recruited to telomeres by TRF1 and provides a critical link between TRF1 and telomerase inhibition to prevent telomere elongation and help maintain telomere homeostasis. PMID: 21119197
    31. Suppression of telomerase activity mediated by PinX1 is involved in the Mad1/c-Myc pathway. PMID: 20544396
    32. loss of PinX1 is an adverse independent molecular marker for epithelial ovarian carcinoma patients. PMID: 20367640
    33. these results suggest that the C-terminal fragment of LPTS/PinX1 (LPTS/PinX1(290-328)) contains a telomerase inhibitory domain that is required for the inhibition of telomere elongation and the induction of cell crisis. PMID: 20620128
    34. role in rRNA and small nucleolar RNA maturation, not in telomere elongation inhibition PMID: 12107183
    35. Over-expression of LPTS-L can induce hepatoma cells into crisis due to the reduction of telomerase activity. PMID: 12439923
    36. Data show that liver-related putative tumor suppressor gene (LPTS) mutations occur in hepatocellular carcinoma but are infrequent and of little effect on the telomerase inhibitory function of the protein. PMID: 12508358
    37. molecular cloning of the 5'-flanking region and determination of the transcription initiation site and four DNase I hypersensitive sites PMID: 14984932
    38. in vitro PinX1 binds directly to the hTERT protein subunit, primarily to the hTR-binding domain, as well as to the hTR subunit PMID: 15381700
    39. LOH of PINX1 locus associated with reduced expression of PINX1 in gastric cancer PMID: 15637589
    40. PINX1 may play major role in gastric cancer development and regulate it through different pathways and expression is a sign of cancer development. PMID: 18784941
    41. Results reveal a novel positive role for PinX1 in telomerase/telomere regulations and suggest that the constitutive expression of PinX1 attributes to telomere maintenance by telomerase and tumorigenicity in cancer cells. PMID: 19117989
    42. Results show that hPinX1 regulates the nucleolar accumulation and telomeric association of TRF1. PMID: 19265708
    43. PinX1 is recruited to chromosome periphery by Nucleolin and a complex of PinX1 and Nucleolin is essential for faithful chromosome congression. PMID: 19393617
    44. The results indicate that PinX1 plays an important role in faithful chromosome segregation in mitosis. PMID: 19553660

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  • 亚细胞定位：
    Nucleus. Nucleus, nucleolus. Chromosome, telomere. Chromosome, centromere, kinetochore. Note=Localizes in nucleoli, at telomere speckles and to the outer plate of kinetochores. Localization to the kinetochore is mediated by its central region and depends on NDC80 and CENPE.
  • 蛋白家族：
    PINX1 family
  • 组织特异性：
    Ubiquitous; expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines.
  • 数据库链接：

    HGNC: 30046

    OMIM: 606505

    KEGG: hsa:54984

    STRING: 9606.ENSP00000318966

    UniGene: Hs.490991