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  4. Recombinant Human Nucleotide triphosphate diphosphatase NUDT15 (NUDT15)

Recombinant Human Nucleotide triphosphate diphosphatase NUDT15 (NUDT15)

  • 中文名称：
    人NUDT15重组蛋白
  • 货号：
    CSB-YP885733HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人NUDT15重组蛋白
  • 货号：
    CSB-EP885733HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人NUDT15重组蛋白
  • 货号：
    CSB-EP885733HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人NUDT15重组蛋白
  • 货号：
    CSB-BP885733HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人NUDT15重组蛋白
  • 货号：
    CSB-MP885733HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NUDT15
  • Uniprot No.：
  • 别名：
    8-dihydro-8-oxoguanine triphosphatase NUDT15; 8-oxo-dGTPase NUDT15; A730068G11Rik; EC 3.1.6.-; FLJ10956; MGC104352; MTH2; MutT homolog 2; Nucleoside diphosphate-linked moiety X motif 15; NUD15_HUMAN; Nudix (nucleoside diphosphate linked moiety X)-type motif ; Nudix motif 15; NUDT15; OTTHUMP00000018389; Probable 7; Probable 7,8-dihydro-8-oxoguanine triphosphatase NUDT15; RP11-90M2.1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-164
  • 氨基酸序列
    MTASAQPRGR RPGVGVGVVV TSCKHPRCVL LGKRKGSVGA GSFQLPGGHL EFGETWEECA QRETWEEAAL HLKNVHFASV VNSFIEKENY HYVTILMKGE VDVTHDSEPK NVEPEKNESW EWVPWEELPP LDQLFWGLRC LKEQGYDPFK EDLNHLVGYK GNHL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May catalyze the hydrolysis of nucleoside triphosphates including dGTP, dTTP, dCTP, their oxidized forms like 8-oxo-dGTP and the prodrug thiopurine derivatives 6-thio-dGTP and 6-thio-GTP. Could also catalyze the hydrolysis of some nucleoside diphosphate derivatives. Hydrolyzes oxidized nucleosides triphosphates like 8-oxo-dGTP in vitro, but the specificity and efficiency towards these substrates are low. Therefore, the potential in vivo sanitizing role of this enzyme, that would consist in removing oxidatively damaged forms of nucleosides to prevent their incorporation into DNA, is unclear. Through the hydrolysis of thioguanosine triphosphates may participate in the catabolism of thiopurine drugs. May also have a role in DNA synthesis and cell cycle progression by stabilizing PCNA. Exhibits decapping activity towards dpCoA-capped RNAs in vitro.
  • 基因功能参考文献：
    1. Results show that 6-TGN levels were substantially low in patients with NUDT15 variants who showed highest hematopoietic toxicity. The low ratio of 6-TGN level to 6-mercaptopurine dose in patients with NUDT15 variants seems to be caused by enhanced incorporation of active thiopurine metabolites into DNA. PMID: 28903549
    2. Mutation rate of NUDT15 in Chinese inflammatory bowel disease patients is higher than that of TPMT. NUDT15 polymorphism is a better predictor for AZA-induced leukopenia than TPMT polymorphism. PMID: 29491687
    3. Mutations in exon 1 of NUDT15 also affect thiopurine-induced leukopenia in patients with inflammatory bowel disease. PMID: 29398872
    4. With increasing copy numbers of the risk T allele at NUDT15. PMID: 29210335
    5. results of this meta-analysis confirm that NUDT15 c.415C>T may be an important predictor of thiopurine-induced leukocytopenia in Asians PMID: 28470355
    6. NUDT15 c.415C>T, c.36_37insGGAGTC, and c.52G>A variants were risk factors for thiopurine-induced leukopenia. Combined detection of the 3 variants could increase the predictive sensitivity of thiopurine-induced leukopenia and help to distinguish early leukopenia in heterozygote of c.415C>T in Chinese patients with inflammatory bowel disease. PMID: 28570428
    7. Study replicated previous findings that the NUDT15 p.R139C variant is a potential predictor for AZA-induced leukopenia, extending this finding to patients with various neurological disorders in Korea and identifying its specific association with early leukopenia and severe alopecia. All of the patients who carried a NUDT15 p.R139C homozygous variant exhibited rapid development of severe leukopenia and extensive hair loss. PMID: 28566182
    8. Thiopurine treatment should not be recommended to patients with NUDT15 homozygous variant genotype due to severe early leukopenia PMID: 29206869
    9. All patients with both NUDT15 rs116855232 heterozygous variants and ABCC4 rs3765534 variants suffered from severe leukopenia and required 6-mercaptopurine dose reduction to less than 35 mg/m(2)/da PMID: 28883280
    10. Current review highlights the scientific data on NUDT15 enzyme variant in patients with acute lymphoblastic leukaemia and its relation to 6-mercaptopurine toxicity in various ethnic populations. PMID: 28963908
    11. 3 novel NUDT15 coding variants (p.R34T, p.K35E, and p.G17_V18del) in 5 children with acute lymphoblastic leukemia enrolled in frontline protocols in Singapore, Taiwan, and at St. Jude Children's Research Hospital. PMID: 28659275
    12. NUDT15 variant as a predictor for thiopurine-induced toxicity in Indian patients. PMID: 27416873
    13. NUDT15 gene polymorphism is related to mercaptopurine intolerance in Taiwan Chinese children with acute lymphoblastic leukemia. PMID: 26503813
    14. our results defined how NUDT15 limits thiopurine efficacy and how genetic ablation via the R139C missense mutation confers sensitivity to thiopurine treatment in patients PMID: 27530327
    15. NUDT15 polymorphisms are associated with 6-mercaptopurine intolerance in children treated for acute lymphoblastic leukemia. PMID: 27577869
    16. NUDT15 c.415C>T may be another predictor of AZA-induced leukocytopenia. PMID: 27381176
    17. our study shows that 6-MP reduction is significant in the younger age group in relation to NUDT15 variant PMID: 27193222
    18. NUDT R139C T/T genotype showed complete association with early severe hair loss/leukopenia in Japanese patients with inflammatory bowel diseases. PMID: 26076924
    19. Letter: discuss role of NUDT15 in complex thiopurine metabolism. PMID: 27308664
    20. There was a close association of NUDT15 R139C with early leukopenia associated with thiopurines in patients in Hong Kong PMID: 27095468
    21. These results suggest that NUDT15 R139C-related thiopurine-induced leukocytopenia is mediated by a 6-TGN-independent mechanism. PMID: 26590936
    22. Mutations of the NUDT15 and TPMT gene accounted for approximately 88% of cases with thiopurine-induced early leukopenia. Extensive hair loss was a recognizable early symptom in patients with the homozygous NUDT15 c.415C>T variant. PMID: 26735160
    23. NUDT15 c.415C>T increases risk of 6-mercaptopurine induced myelosuppression during maintenance therapy in children with acute lymphoblastic leukemia. PMID: 26405151
    24. Patients with defective NUDT15 alleles showed excessive levels of thiopurine active metabolites and toxicity. [meta-analysis] PMID: 26878724
    25. Depletion of NUDT15 has no effect on incorporation of 8-oxo-dGTP into DNA and does not impact cancer cell survival in cell lines tested. NUDT15 is not a biologically relevant 8-oxo-dGTPase PMID: 26238318
    26. Event-free survival did not significantly differ by NUDT15 genotype. rs116855232 is an important determinant of 6-MP myelotoxicity in Japanese children with ALL and may represent the most robust toxicity-related locus in Asians to date. PMID: 26033531
    27. Describe a germline variant in NUDT15 strongly associated with mercaptopurine intolerance in childhood acute lymphoblastic leukemia, which may have implications for treatment individualization in this disease. PMID: 25624441
    28. Authros identified a nonsynonymous SNP in NUDT15 (encoding p.Arg139Cys) that was strongly associated with thiopurine-induced early leukopenia (odds ratio (OR) = 35.6; P(combined) = 4.88 x 10(-94)). PMID: 25108385
    29. human MTH1, MTH2, and NUDT5 proteins act as a defense against the mutagenesis induced by oxidized dGTP. PMID: 20144704
    30. Data demonstrate for the first time that PCNA is protected by this newly identified partner molecule MTH2, which is related to DNA synthesis and cell cycle progression. PMID: 19419956

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  • 蛋白家族：
    Nudix hydrolase family
  • 数据库链接：

    HGNC: 23063

    OMIM: 615792

    KEGG: hsa:55270

    STRING: 9606.ENSP00000258662

    UniGene: Hs.144407