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  4. Recombinant Human N-cym protein (MYCNOS)

Recombinant Human N-cym protein (MYCNOS)

  • 中文名称：
    人MYCNOS重组蛋白
  • 货号：
    CSB-YP015279HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人MYCNOS重组蛋白
  • 货号：
    CSB-EP015279HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人MYCNOS重组蛋白
  • 货号：
    CSB-EP015279HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人MYCNOS重组蛋白
  • 货号：
    CSB-BP015279HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人MYCNOS重组蛋白
  • 货号：
    CSB-MP015279HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MYCNOS
  • Uniprot No.：
  • 别名：
    MYCNOS; CYMN; NCYM; N-cym protein; N-myc opposite strand
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-109
  • 氨基酸序列
    MQHPPCEPGN CLSLKEKKIT EGSGGVCWGG ETDASNPAPA LTACCAAERE ANVEQGLAGR LLLCNYERRV VRRCKIAGRG RAPLGTRPLD VSSFKLKEEG RPPCLKINK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulates stability of MYCN in neuroblastoma cells by inhibiting GSK3B-mediated MYCN phosphorylation. Inhibits GSK3B activity by promoting its phosphorylation at 'Ser-9'.
  • 基因功能参考文献：
    1. lncRNA long stress-induced noncoding transcript 5 (LSINCT5) is significantly upregulated in human bladder cancer cell lines and tumor specimens. Mechanistic investigations showed that LSINCT5 could physically interact with NCYM, a de novo gene product from the MYCN cis-antisense RNA and inhibit GSK3beta activity leading to enhanced Wnt/beta-catenin signaling activation and epithelial mesenchymal transition (EMT). PMID: 29772237
    2. An alternative transcript of MYCNOS, MYCNOS-01, post-transcriptionally regulates MYCN levels and affects growth in MYCN-amplified rhabdomyosarcoma and neuroblastoma cells. PMID: 29466962
    3. High levels of NCYM expression in human neuroblastoma tissues correlate with high levels of N-Myc, NonO and lncUSMycN expression and poor patient survival, and treatment with BET bromodomain inhibitors reduces NCYM expression, providing further evidence for the application of BET bromodomain inhibitors for the therapy of neuroblastoma characterized by MYCN/NCYM gene locus amplification. PMID: 27748806
    4. functional interplay between MYCN, NCYM, and OCT4 contributes to aggressiveness of MYCN-amplified human neuroblastomas. PMID: 25880909
    5. these results suggest that GSK3b inhibition by NCYM stabilizes the MYCN protein both in vitro and in vivo. PMID: 24391509

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 组织特异性：
    Expressed in the neuronal cells of the cerebrum and cerebellum, spermatocytes of the testis, pancreatic cells and also the heart. Expressed in both primary and metastatic neuroblastomas and in thyroid tumors (at protein level). Expression is associated wi
  • 数据库链接：

    HGNC: 16911

    OMIM: 605374

    KEGG: hsa:10408

    UniGene: Hs.651453