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  4. Recombinant Human Mitochondrial Rho GTPase 1 (RHOT1), partial

Recombinant Human Mitochondrial Rho GTPase 1 (RHOT1), partial

  • 货号：
    CSB-YP814223HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP814223HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP814223HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP814223HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP814223HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    ARHT 1; ARHT1; FLJ11040; FLJ12633; hMiro 1; hMiro-1; hMiro1; MIRO 1; MIRO-1; MIRO1; MIRO1_HUMAN; mitochondrial Rho (MIRO) GTPase 1; Mitochondrial Rho 1; Mitochondrial Rho GTPase 1; Mitochondrial Rho1; Rac GTP binding protein like protein; Rac-GTP-binding protein-like protein; Ras homolog family member T1; Ras homolog gene family member T1; Rhot 1; RHOT1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
  • 基因功能参考文献：
    1. The findings identify for the first time peroxisome-localized Miro1 variants as adapter proteins that link peroxisomes to the microtubule-dependent transport complexes including TRAK2 in the intracellular translocation of peroxisomes in mammalian cells. PMID: 29222186
    2. LPS mediates intercellular tight junction destruction among TECs and RhoT1/SMAD-4/JAM-3 is a pivotal pathway to mediate the phenomenon. PMID: 29725250
    3. a key role of Miro1, which promotes the mitochondrial transfer from multipotent mesenchymal stem cells, is reported. PMID: 29562677
    4. Show that the C-terminal GTPase of the Parkin primary substrates Miro1 and Miro2 are necessary and sufficient for efficient ubiquitination. We present several new X-ray crystal structures of both Miro1 and Miro2 that reveal substrate recognition and ubiquitin transfer to be specific to particular protein domains and lysine residues. PMID: 27605430
    5. Results provide evidence that ALS mutant SOD1 inhibits axonal transport of mitochondria by inducing PINK1/Parkin-dependent Miro1 degradation. PMID: 28973175
    6. prolonged retention of Miro, and the downstream consequences that ensue, may constitute a central component of Parkinson's disease pathogenesis. PMID: 27618216
    7. Full-length APC promotes assembly of the Miro-1/Milton-2 complex. PMID: 26658612
    8. Miro and Cenp-F promote anterograde mitochondrial movement and proper mitochondrial distribution in daughter cells. PMID: 26259702
    9. Data indicate that outer mitochondrial membrane protein Miro1 can stabilize phospho-mutant versions of PARK2 gene (encoding Parkin) on the outer mitochondrial membrane (OMM). PMID: 24671417
    10. Details of a robust association of DISC1 with mitochondrial transport complexes containing TRAK1 and Miro1. PMID: 24092329
    11. The Miro1-mediated mitochondrial transport in neurons and recently highlighted involvement of Miro1 proteins in mitochondrial turnover, emerging as a key process affected in neurodegeneration. PMID: 24256248
    12. CXCL12-dependent cell polarization and migration are reduced in Miro-1-silenced cells PMID: 24492963
    13. results indicated that the low-expression levels of RhoT1 and Smad4 were significantly associated with LNM and shorter survival. RhoT1 may be considered as a potential novel marker for predicting the outcome in patients with pancreatic cancer PMID: 22860091
    14. Study shows that both PINK1 and Parkin halt mitochondrial movement; PINK1 phosphorylates Miro (1 and 2) and thereby initiates the rapid degradation of Miro through a Parkin- and proteasome-dependent pathway. PMID: 22078885
    15. Moreover, we show that Miro interacts with the Kinesin-binding proteins, GRIF-1 and OIP106, suggesting that the Miro GTPases form a link between the mitochondria and the trafficking apparatus of the microtubules. PMID: 16630562
    16. Miro1,2 proteins serve as a [Ca(2+)](c)-sensitive switch and bifunctional regulator for both the motility and fusion-fission dynamics of the mitochondria. PMID: 19098100
    17. These data suggest that Miro1 and the kinesin adaptor Grif-1 play an important role in regulating mitochondrial transport in neurons. PMID: 19103291

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  • 亚细胞定位：
    Mitochondrion outer membrane; Single-pass type IV membrane protein. Note=Colocalizes with MGARP and RHOT2 at the mitochondria.
  • 蛋白家族：
    Mitochondrial Rho GTPase family
  • 组织特异性：
    Ubiquitously expressed. Expressed at high level in heart and skeletal muscle.
  • 数据库链接：

    HGNC: 21168

    OMIM: 613888

    KEGG: hsa:55288

    STRING: 9606.ENSP00000351132

    UniGene: Hs.655325