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  4. Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor (MANF)

Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor (MANF)

  • 中文名称：
    人MANF重组蛋白
  • 货号：
    CSB-YP002118HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人MANF重组蛋白
  • 货号：
    CSB-EP002118HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人MANF重组蛋白
  • 货号：
    CSB-EP002118HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人MANF重组蛋白
  • 货号：
    CSB-BP002118HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人MANF重组蛋白
  • 货号：
    CSB-MP002118HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MANF
  • Uniprot No.：
  • 别名：
    Arginine rich mutated in early stage tumors; Arginine rich protein; Arginine-rich protein; ARMET; ARMET protein; ARP; Manf; MANF_HUMAN; Mesencephalic astrocyte derived neurotrophic factor; Mesencephalic astrocyte-derived neurotrophic factor; MGC142148; MGC142150; Protein ARMET
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-182
  • 氨基酸序列
    LRPGDC EVCISYLGRF YQDLKDRDVT FSPATIENEL IKFCREARGK ENRLCYYIGA TDDAATKIIN EVSKPLAHHI PVEKICEKLK KKDSQICELK YDKQIDLSTV DLKKLRVKEL KKILDDWGET CKGCAEKSDY IRKINELMPK YAPKAASART DL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions. Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia. Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells. Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions.
  • 基因功能参考文献：
    1. Our data indicate that increased MANF concentrations in serum are associated with the clinical manifestation of type 1 diabetes in children, but the exact mechanism behind the increase remains elusive PMID: 27356471
    2. Sulfatide binding promotes cellular MANF uptake and cytoprotection from hypoxia-induced cell death. PMID: 29497057
    3. Serum MANF level was higher in patients with newly diagnosed prediabetes and T2 Diabetes Mellitus than in normal glucose tolerance controls. MANF appears to be associated with Matsuda Index, QUICKI and HOMA-IR in prediabetes patients. PMID: 28216543
    4. the role of two short sequence motifs within the carboxy-(C) terminal domain of MANF in its neuroprotective activity, was studied. PMID: 26720341
    5. the selective expression of MANF in splenocytes may be involved in plasma cell differentiation and immune regulation. PMID: 26429332
    6. MANF is a protein that interacts with RTN1-C PMID: 25543119
    7. We demonstrate that Armet and Creld2 are genotype-specific ER stress response proteins with substrate specificities, and that aggregation of mutant matrilin-3 is a key disease trigger in MED that could be exploited as a potential therapeutic target PMID: 23956175
    8. MANF binding to the plasma membrane also required the RTDL sequence and was inhibited with a peptide known to interact with KDELRs, suggesting MANF binds KDELRs at the surface. PMID: 23255601
    9. MANF and C-MANF protect neurons intracellularly as efficiently as Ku70. PMID: 21047780
    10. Pretreatment with adeno-associated-virus vector containing human MANF reduces the volume of cerebral infarction and facilitates behavioral recovery in experimental stroke rats. PMID: 20685313
    11. Armet is a novel secreted mediator of the adaptive pathway of unfolded protein response. PMID: 18561914
    12. The widespread expression of MANF together with its evolutionary conserved nature and regulation by brain insults suggest that it has important functions both under normal and pathological conditions in many tissue types. PMID: 18718866
    13. structures of MANF and CDNF were solved; structure explains why MANF and CDNF are bifunctional; neurotrophic activity may reside in the N-terminal domain and ER stress response in the C-terminal domain PMID: 19258449

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  • 亚细胞定位：
    Secreted. Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen.
  • 蛋白家族：
    ARMET family
  • 数据库链接：

    HGNC: 15461

    OMIM: 601916

    KEGG: hsa:7873

    STRING: 9606.ENSP00000432799

    UniGene: Hs.436446