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  4. Recombinant Human Melanoma-associated antigen 11 (MAGEA11)

Recombinant Human Melanoma-associated antigen 11 (MAGEA11)

  • 中文名称：
    人MAGEA11重组蛋白
  • 货号：
    CSB-YP013325HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人MAGEA11重组蛋白
  • 货号：
    CSB-EP013325HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人MAGEA11重组蛋白
  • 货号：
    CSB-EP013325HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人MAGEA11重组蛋白
  • 货号：
    CSB-BP013325HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人MAGEA11重组蛋白
  • 货号：
    CSB-MP013325HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MAGEA11
  • Uniprot No.：
  • 别名：
    MAGEA11; MAGE11Melanoma-associated antigen 11; Cancer/testis antigen 1.11; CT1.11; MAGE-11 antigen
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-429
  • 氨基酸序列
    METQFRRGGL GCSPASIKRK KKREDSGDFG LQVSTMFSED DFQSTERAPY GPQLQWSQDL PRVQVFREQA NLEDRSPRRT QRITGGEQVL WGPITQIFPT VRPADLTRVI MPLEQRSQHC KPEEGLQAQE EDLGLVGAQA LQAEEQEAAF FSSTLNVGTL EELPAAESPS PPQSPQEESF SPTAMDAIFG SLSDEGSGSQ EKEGPSTSPD LIDPESFSQD ILHDKIIDLV HLLLRKYRVK GLITKAEMLG SVIKNYEDYF PEIFREASVC MQLLFGIDVK EVDPTSHSYV LVTSLNLSYD GIQCNEQSMP KSGLLIIVLG VIFMEGNCIP EEVMWEVLSI MGVYAGREHF LFGEPKRLLT QNWVQEKYLV YRQVPGTDPA CYEFLWGPRA HAETSKMKVL EYIANANGRD PTSYPSLYED ALREEGEGV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

We are interested in the following proteins expressed in mammalian with a concentration of 50ug/ml. Please let me know if this is possible for the following products:
We would also like to know if it’s possible to buy some of the antigen resuspension buffer you use?
We will be using these proteins to build a protein array for serum profiling and are unsure if they need aseptic processing or endotoxin removal?
Can you please advise whether this is necessary? We would like these proteins in eukaryotic host instead with a concentration of 50ug/ml.
CSB-MP009188HU
CSB-MP768782HU
CSB-MP013325HU
CSB-MP887003HU
CSB-MP013348HU
CSB-MP636766XBF
CSB-MP897569HU
CSB-MP009178HU
CSB-MP664879BO

A:
1.Thanks for your inquiry.
Recombinant Human Melanoma-associated antigen 11(MAGEA11)
CSB-YP013325HU >> Yeast
CSB-EP013325HU >> E.coli
CSB-BP013325HU >> Baculovirus
CSB-MP013325HU >> Mammalian cell
Expression Region: 1-429aa; Full length.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is listed as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

METQFRRGGLGCSPASIKRKKKREDSGDFGLQVSTMFSEDDFQSTERAPYGPQLQWSQDLPRVQVFREQANLEDRSPRRTQRITGGEQVLWGPITQIFPTVRPADLTRVIMPLEQRSQHCKPEEGLQAQEEDLGLVGAQALQAEEQEAAFFSSTLNVGTLEELPAAESPSPPQSPQEESFSPTAMDAIFGSLSDEGSGSQEKEGPSTSPDLIDPESFSQDILHDKIIDLVHLLLRKYRVKGLITKAEMLGSVIKNYEDYFPEIFREASVCMQLLFGIDVKEVDPTSHSYVLVTSLNLSYDGIQCNEQSMPKSGLLIIVLGVIFMEGNCIPEEVMWEVLSIMGVYAGREHFLFGEPKRLLTQNWVQEKYLVYRQVPGTDPACYEFLWGPRAHAETSKMKVLEYIANANGRDPTSYPSLYEDALREEGEGV


2. As you want these proteins expressed in mammalian with a concentration of 50ug/ml, generally the concentration for MP protein is 0.1-0.5mg/ml (100ug/ml-500ug/ml),
The concentration of 50ug/ml is a little low, the low concentration is not very good for the protein stability, so we recommend the concentration is better higher than 0.1mg/ml.
3. You would also like to know if it’s possible to buy some of the antigen resuspension buffer you use?
How much do you need for the buffer ? If it's not too much, we can provide some for free, such as 5ml or 10ml.
4.You will be using these proteins to build a protein array for serum profiling and are unsure if they need aseptic processing or endotoxin removal?
Can you please advise whether this is necessary? You would like these proteins in eukaryotic host instead with a concentration of 50ug/ml.
According to your application, they don't need aseptic processing or endotoxin removal.
Do you mean that the concentration of 50ug/ml is not necessary, as long as these proteins are expressed in eukaryotic host ?
If yes, we recommend the concentration is better higher than 100ug/ml, and you can choose any eukaryotic expression systems. (YP, BP, MP)

靶点详情

  • 功能：
    Acts as androgen receptor coregulator that increases androgen receptor activity by modulating the receptors interdomain interaction. May play a role in embryonal development and tumor transformation or aspects of tumor progression.
  • 基因功能参考文献：
    1. SUV39H2 functioned cooperatively with MAGE-A11 to increase androgen-dependent AR transcriptional activity. PMID: 28042025
    2. direct interactions of MAGE-A11 with Skp2 and cyclin A regulate the substrate-specificity of Skp2-mediated protein degradation. PMID: 27720894
    3. MAGE-A11 and androgen receptor cooperate in the up-regulation of FSTL1 to promote the growth of prostate cancer. PMID: 27976415
    4. MAGE-A11 is an independent poor prognostic marker for esophageal squamous cell carcinoma (ESCC) patients. MAGE-A11 regulates various cell functions and directly increases the invasion and proliferation of ESCC cells. PMID: 27362547
    5. degradation of MAGE-A11 promoted by the human p14-ARF tumor suppressor contributes to low levels of MAGE-A11 in nontransformed cells PMID: 26330556
    6. MAGE-A11, similarly to HER-2 and ER-beta, may be an important diagnostic or prognostic indicator in breast cancer and potentially promotes tumor proliferation. PMID: 23064813
    7. MAGEA11 regulation is highly instructive for understanding mechanisms regulating CG antigen genes in human cancer. PMID: 23839233
    8. MAGE-A11 is a proto-oncogene whose increased expression in prostate cancer reverses retinoblastoma-related protein p107 from a transcriptional repressor to a transcriptional activator of the androgen receptor and E2F1. PMID: 23853093
    9. MAGE-A11 increases AR transcriptional activity by linking androgen receptor dimers. PMID: 23172223
    10. the functional defect associated with an AR exon 1 missense mutation. PMID: 22334658
    11. Gain in transcriptional activity by primate-specific coevolution of melanoma antigen-A11 and its interaction site in androgen receptor. PMID: 21730049
    12. MAGE-11 links NH(2)-terminal domains of AR and p300 to promote transcriptional synergy through a cadre of FXXLF-related interacting motifs PMID: 20448036
    13. Results suggest that MAGE-11 functions as a bridging factor to recruit androgen receptor coactivators through a novel FXX(L/I)F motif-F-box interaction paradigm. PMID: 19828458
    14. MAGE-11 is a unique AR coregulator that increases AR activity by modulating the AR interdomain interaction. PMID: 15684378
    15. The results suggest that MAGE-11 functions as a coregulator that increases AR transcriptional activity during the establishment of uterine receptivity in the human female. PMID: 18048459
    16. The time-dependent EGF-induced increase in AR transcriptional activity by MAGE-11 is mediated through AR activation functions 1 and 2 in association with the increased turnover of AR and MAGE-11. PMID: 18212060
    17. Knockdown of MAGE-11 by small interfering RNA results in decreased hypoxic induction of HIF-1alpha and its target genes. Inhibition of PHD by MAGE-11, and following activation of HIFs, is a novel tumor-associated HIF regulatory mechanism. PMID: 19147576
    18. Increased expression of the AR coregulator MAGE-11 through promoter DNA hypomethylation and cyclic AMP is associated with prostate cancer PMID: 19372581

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 组织特异性：
    Expressed in tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma. Expressed in testis, ovary, prostate, cancerous prostate, breast and adrenal tissue.
  • 数据库链接：

    HGNC: 6798

    OMIM: 300344

    KEGG: hsa:4110

    STRING: 9606.ENSP00000347358

    UniGene: Hs.670252