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  4. Recombinant Human LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1)

Recombinant Human LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1)

  • 货号：
    CSB-YP012955HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP012955HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP012955HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP012955HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP012955HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    LIMS1
  • Uniprot No.：
  • 别名：
    2310016J22Rik; 4921524A02Rik; AI507642; AU021743; AW551584; C430041B13Rik; LIM and senescent cell antigen like domains 1; LIM and senescent cell antigen-like-containing domain protein 1; LIM zinc finger domain containing 1; LIM-type zinc finger domains 1; Lims1; LIMS1_HUMAN; Lims1l; Particularly interesting new Cys His protein 1; Particularly interesting new Cys His protein; Particularly interesting new Cys-His protein 1; PINCH 1; PINCH; PINCH-1; PINCH1; Renal carcinoma antigen NY REN 48 ; Renal carcinoma antigen NY-REN-48; senescent cell antigen; wu:fc32c03; zgc:112533
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-325
  • 氨基酸序列
    ANALASATC ERCKGGFAPA EKIVNSNGEL YHEQCFVCAQ CFQQFPEGLF YEFEGRKYCE HDFQMLFAPC CHQCGEFIIG RVIKAMNNSW HPECFRCDLC QEVLADIGFV KNAGRHLCRP CHNREKARGL GKYICQKCHA IIDEQPLIFK NDPYHPDHFN CANCGKELTA DARELKGELY CLPCHDKMGV PICGACRRPI EGRVVNAMGK QWHVEHFVCA KCEKPFLGHR HYERKGLAYC ETHYNQLFGD VCFHCNRVIE GDVVSALNKA WCVNCFACST CNTKLTLKNK FVEFDMKPVC KKCYEKFPLE LKKRLKKLAE TLGRK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation.
  • 基因功能参考文献：
    1. High LIMS1 expression is associated with Neuroblastoma. PMID: 29695398
    2. focal adhesion signaling to actin cytoskeleton is implicated in human laryngeal carcinogenesis and PINCH1 has prognostic significance in the disease. PMID: 29755929
    3. that PINCH-1 may be playing an important role in etiopathogenesis of both subtypes breast cancer PMID: 29079319
    4. Mammalian cells have two functional PINCH proteins, PINCH1 and PINCH2. PINCH not only binds to Nck2 and engages in the signaling of growth factor receptors, but also forms a ternary complex with ILK and parvin (IPP complex). PMID: 27590440
    5. our data suggest an essential role of PINCH1, ILK and ILKAP for the radioresistance of p53-wildtype glioblastoma multiforme cells PMID: 26460618
    6. Data suggest that PINCH1 and Nck2 critically participate in the regulation of cellular radiosensitivity and EGFR function and downstream signaling in a cellular model of human squamous cell carcinoma. PMID: 26004008
    7. Downregulation of PINCH1 is associated with metastatic breast cancer. PMID: 25647720
    8. changes in CSF levels of PINCH appear to correlate with changes in blood CD4 count and with changes in CSF hyperphosphorylated Tau levels PMID: 24817145
    9. PINCH predicts survival in rectal cancer patients with RT, but not in patients without RT. The expression of PINCH may be regulated by radiation and by environmental factors surrounding the cells. PMID: 23970013
    10. PINCH is increased and binds to hp-Tau. These studies address a new mechanism by which AD and HIV may intersect and identify PINCH as a contributing factor to the accumulation of hyperphosphorylated Tau. PMID: 23554879
    11. Pinch-1 mRNA and protein were significantly up-regulated in acute lymphoblastic leukemia and acute myeloid leukemia bone marrow stromal cells compared to normal bone marrow stromal cells (p<0.01). PMID: 22310984
    12. PINCH protein might play an important role in the tumourigenesis and metastasis of gastric adenocarcinoma. PMID: 22976000
    13. PINCH mRNA overexpression in colorectal carcinomas is correlated with VEGF and FAS mRNA expression PMID: 22199270
    14. PINCH may function as a neuron-specific host-mediated response to challenge by HIV-related factors in the CNS. PMID: 20689998
    15. PINCH1 can shuttle into the nucleus from cytoplasm in podocytes, wherein it interacts with WT1 and suppresses podocyte-specific gene expression. PMID: 21390327
    16. Review provides an overview of the current knowledge of the molecular interactions of PINCH with other components of focal adhesions and discusses its potential implication for human heart disease. PMID: 19952891
    17. data reveal how specific domains of PINCH-1 direct two independent pathways: one utilizing ILK to allow cell attachment, and the other recruiting Rsu-1 to activate Rac1 in order to promote cell spreading PMID: 20926685
    18. PINCH staining at the tumour invasive margin was related to survival in poorly differentiated tumours but not in better differentiated tumours, indicating that the impact of PINCH on prognosis was dependent on differentiation status. PMID: 21426571
    19. Data suggest that the adapter protein PINCH1 critically participates in the regulation of the cellular radiosensitivity of normal and malignant cells similarly under adhesion and suspension conditions. PMID: 20927395
    20. Findings of increased PINCH protein in more advanced stages of human pancreatic ductal adenocarcinoma, as well as in metastatic tumours in the animal model, support the hypothesis that PINCH is an important controller of cell survival and migration. PMID: 20590912
    21. PINCH expression markedly increased in tumor-associated stroma in endometrioid carcinoma compared to normal endometrium and atypical endometrial hyperplasia; results suggest PINCH seems to play a role in tumorigenesis and development of endometrial cancer PMID: 20714146
    22. Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites PMID: 12432066
    23. PINCH1 and ILK are essential for prompt cell spreading and motility; PINCH1 and ILK, like alpha-parvin, are crucial for cell survival; PINCH1 and ILK are required for optimal activating phosphorylation of PKB/Akt of the survival pathway PMID: 14551191
    24. PINCH-1 functions as a molecular platform for coupling and uncoupling diverse cellular processes via overlapping but yet distinct domain interactions PMID: 15941716
    25. Up-regulation of PINCH protein in stroma may be involved in promoting invasion and metastasis in oral squamous cell cacinoma. PMID: 16273248
    26. PINCH-1, through its interaction with integrin-linked kinase, plays important role in regulating TGF-beta1-mediated epithelial-to-mesenchymal transition and could be potential future therapeutic target to prevent progression of renal disease. PMID: 17656471
    27. PINCH-1 contributes to apoptosis resistance through suppression of Bim. Mechanistically, PINCH-1 suppresses Bim not only transcriptionally but also post-transcriptionally. PMID: 18063582
    28. PINCH expression may be involved in glioma development and differentiation. PMID: 18187956
    29. observations that PINCH is robustly expressed in the CNS of HIV patients suggests an important role for PINCH in HIV-associated neurodegenerative processes PMID: 18459134
    30. PINCH protein is overexpressed in tumor-associated stroma of esophageal squamous cell carcinoma. PMID: 18957717
    31. zincs are coordinated by PINCH1 LIM1, and suggests that conformational flexibility and twisting between the 2 zinc fingers within the LIM1 domain may be important for ILK binding. PMID: 19074270

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  • 亚细胞定位：
    Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
  • 组织特异性：
    Expressed in most tissues except in the brain.
  • 数据库链接：

    HGNC: 6616

    OMIM: 602567

    KEGG: hsa:3987

    STRING: 9606.ENSP00000446121

    UniGene: Hs.597715