Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Human Interleukin-1 receptor-associated kinase 1 (IRAK1)

Recombinant Human Interleukin-1 receptor-associated kinase 1 (IRAK1)

  • 中文名称：
    人IRAK1重组蛋白
  • 货号：
    CSB-YP011808HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人IRAK1重组蛋白
  • 货号：
    CSB-EP011808HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人IRAK1重组蛋白
  • 货号：
    CSB-EP011808HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人IRAK1重组蛋白
  • 货号：
    CSB-BP011808HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人IRAK1重组蛋白
  • 货号：
    CSB-MP011808HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    AA48924; Il1rak; Interleukin 1 receptor associated kinase 1; Interleukin-1 receptor-associated kinase 1; IRAK; IRAK-1; Irak1; IRAK1-S; IRAK1_HUMAN; mPLK; OTTHUMP00000026014; OTTHUMP00000026015; OTTHUMP00000026020; OTTHUMP00000180621; Pelle; Pelle homolog; Pelle-like protein kinase; Plpk
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-712
  • 氨基酸序列
    MAGGPGPGEP AAPGAQHFLY EVPPWVMCRF YKVMDALEPA DWCQFAALIV RDQTELRLCE RSGQRTASVL WPWINRNARV ADLVHILTHL QLLRARDIIT AWHPPAPLPS PGTTAPRPSS IPAPAEAEAW SPRKLPSSAS TFLSPAFPGS QTHSGPELGL VPSPASLWPP PPSPAPSSTK PGPESSVSLL QGARPFPFCW PLCEISRGTH NFSEELKIGE GGFGCVYRAV MRNTVYAVKR LKENADLEWT AVKQSFLTEV EQLSRFRHPN IVDFAGYCAQ NGFYCLVYGF LPNGSLEDRL HCQTQACPPL SWPQRLDILL GTARAIQFLH QDSPSLIHGD IKSSNVLLDE RLTPKLGDFG LARFSRFAGS SPSQSSMVAR TQTVRGTLAY LPEEYIKTGR LAVDTDTFSF GVVVLETLAG QRAVKTHGAR TKYLKDLVEE EAEEAGVALR STQSTLQAGL AADAWAAPIA MQIYKKHLDP RPGPCPPELG LGLGQLACCC LHRRAKRRPP MTQVYERLEK LQAVVAGVPG HSEAASCIPP SPQENSYVSS TGRAHSGAAP WQPLAAPSGA SAQAAEQLQR GPNQPVESDE SLGGLSAALR SWHLTPSCPL DPAPLREAGC PQGDTAGESS WGSGPGSRPT AVEGLALGSS ASSSSEPPQI IINPARQKMV QKLALYEDGA LDSLQLLSSS SLPGLGLEQD RQGPEESDEF QS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.
  • 基因功能参考文献：
    1. The overexpression of miR-146a significantly increased the ErbB4 expression, decreased the expression of TRAF6, IRAK1, caspase 3, and the phosphorylation level of NF-kappaB, and also increased the Bcl-2/Bax ratio, suggesting the inhibition of inflammation and apoptosis. PMID: 30224945
    2. our work extends the knowledge that IRAK1 is overexpressed and overactivated in CD4+ T cells from patients with SLE. The data highlight that when IRAK1 is blocked the differentiation of lupus naive CD4+ T cells into Th17 cells is repressed, suggesting therapeutic potential against SLE. PMID: 29611775
    3. These collective data indicated that IRAK1 overexpression promotes endometrial carcinoma tumorigenesis by activating mitotic cell cycle and cell division pathways PMID: 28980703
    4. Study identified IRAK1 and TRAF6 are direct targets of miR-146a in cervical cancer cells. Their expression is downregulated by miR-146a to promote cell viability. PMID: 29693168
    5. The high mRNA levels of IRAK1 and IRAK4 were correlated with the development of Behcet's disease, which suggested that IRAK1 and IRAK4 might participate in the pathogenesis of Behcet's disease PMID: 28780618
    6. MiRNA-146a rs2910164 and IRAK1 rs3027898 polymorphisms were a risk factor for predisposition to Rheumatoid Arthritis in Egyptian Population in codominant and dominant tested inheritance models, while, the miRNA-499 rs3746444 and PADI4 rs1748033 polymorphisms were a risk factor in codominant and recessive one. PMID: 29734142
    7. Results show that the expression level of IRAK1 protein in papillary thyroid carcinoma (PTC) tissues was significantly decreased. Also, its expression level is regulated by miR-146a and miR-146b. PMID: 29048684
    8. miR-146a suppresses the inflammatory response in human white adipocytes via targeting the expression of IRAK1 and TRAF6. PMID: 27922090
    9. NLRC3 promoted K48-linked polyubiquitination and degradation of interleukin-1 receptor-associated kinase 1 (IRAK1). PMID: 28731142
    10. miRNA146a expression was significantly higher in hepatitis C patients with a best cut off value 1.63 to discriminate between hepatitis C patients and healthy controls. Meanwhile, it was negatively correlated to IRAK1 and TRAF6 levels and positively correlated to viral load in hepatitis C patients. PMID: 28587864
    11. These findings suggest that human IRAK-1 is essential downstream from TLRs but not IL-1Rs in fibroblasts, whereas it plays a redundant role downstream from both TLRs and IL-1Rs in leukocytes. PMID: 28069966
    12. Expression of X-linked Toll-like receptor 4 signaling gene IRAK1 was significantly elevated in female neonates vs. male neonates. PMID: 28060792
    13. The s demonstrated that EV71 infection upregulates miR-21, which in turn suppresses EV71-triggered type I IFN production, thus promoting EV71 replication. Furthermore, they demonstrated that miR-21 targets the myeloid differentiation factor 88(MyD88) and interleukin-1 receptor-associated kinase 1(IRAK1), which are involved in EV71-induced type I IFN production. PMID: 28506791
    14. Study shows that IRAK1 was over-expressed in human hepatocellular carcinoma (HCC) tumor tissues and provides evidence that IRAK1 promotes cell proliferation and protects against apoptosis in HCC. PMID: 27619757
    15. These results suggest that the IRAK1-binding protein OPTN negatively regulates IL-1beta/LPS-induced NF-kappaB activation by preventing polyubiquitination of TRAF6. PMID: 28882891
    16. IRAK1 regulates lipid accumulation by modulating CD36-mediated uptake and ABCA1-, ABCG1-dependent cholesterol efflux. PMID: 27270491
    17. High IRAK1 expression is associated with multiple myeloma. PMID: 27454822
    18. TLR4- and TLR2-induced IRAK-ERK pathway cross-talks with p67phox-Nox-2 for reactive oxygen species generation, thus regulating IL-1beta transcription and processing in monocytic cells. PMID: 26320741
    19. Data suggest that, in monocytes and macrophages, the interleukin-1B- (IL1B)-stimulated trans-autophosphorylation of IRAK4 (interleukin-1 receptor-associated kinase 4) is initiated by MYD88- (myeloid differentiation primary response gene 88)-induced dimerization of IRAK4. In contrast, IRAK1 is inactive in unstimulated monocytes/macrophages and is converted to an active protein kinase in response to IL1B. PMID: 28512203
    20. Data indicate the complexity of interactions between Pin1 and activated IRAK1, suggesting that phosphorylation of neighboring Ser/Thr-Pro motifs in proteins might provide competitive advantage at cellular concentrations for engaging with Pin1. PMID: 27790836
    21. IRAK1 is a direct target of miR-146b and has functional roles to inhibit various aggressive papillary thyroid carcinoma cell activities. PMID: 27533309
    22. our data strongly suggest that AQCA-mediated suppression of inflammatory responses could be managed by a direct interference of signaling cascades including IRAK and Syk, linked to the activation of NF-kappaB and AP-1. PMID: 27338330
    23. The IRAK1 rs3027898 was not associated with RA, whereas C allele of miR-146a rs2910164 was found to be protective. PMID: 28207326
    24. the Xq28 region containing TMEM187 and IRAK1 (rs13397, rs1059703, and rs1059702) with rheumatoid arthritis (RA)susceptibility; results replicated association of the 3 Xq28 polymorphisms with RA risk in Tunisian and French populations and suggested that RA susceptibility is associated with TMEM187-IRAK1 polymorphisms in women PMID: 28271077
    25. IRAK1 expression was not significantly increased in tympanic membrane of otitis media patients. PMID: 27497395
    26. Our findings reveal that IRAK1 promotes cell survival and is an attractive therapeutic target in head and neck squamous cell carcinomas PMID: 26527316
    27. miR146a has an important promoting effect on the apoptosis of granulosa cells by targeting IRAK1 and TRAF6 via the caspase cascade pathway PMID: 26151128
    28. This is the first study to show an association between single nucleotide polymorphisms in IRAK1, IRAK4 and MyD88, and the presence of severe invasive pneumococcal disease. PMID: 26075815
    29. This meta-analysis suggests that the miR-499 rs374644 and IRAKI rs3027898 polymorphisms are associated with susceptibility to inflammatory arthritis. [review] PMID: 25269878
    30. IRAK1 overexpression drive aggressive growth, metastasis and acquired resistance to paclitaxel treatment in breast cancer. PMID: 26503059
    31. Data suggest that TLR2 (toll like receptor 2) is down-regulated by microRNA-UL112-3p from human Cytomegalovirus; microRNA-UL112-3p also down-regulates TLR2-induced post-translational activation of IRAK1 signaling. PMID: 25955717
    32. These data demonstrated that these three single nucleotide polymorphisms (rs3027898, rs1059702, rs1059703) in IRAK1 were associated with autoimmune diseases risk. PMID: 26142671
    33. Src, Syk, IRAK1, and IRAK4 have roles in anti-inflammatory responses mediated by dietary flavonoid Kaempferol PMID: 25922567
    34. High mRNA levels of IRAK1 and IRAK4 correlated with VKH disease activity. PMID: 24690905
    35. expression of IRAK1 in lung cancer was significantly higher compared with that in normal lung tissues and was correlated with TNM stage, lymphatic metastasis and tumor size. PMID: 25550857
    36. results firstly indicated that IRAK1 and MECP2 genes are crucial risk factors for AITDs. PMID: 25458699
    37. IRAK1 was constitutively phosphorylated in PEL and required for survival, implicating IRAK1 and TLR signaling as a driver pathway in PEL PMID: 25341731
    38. MicroRNA-146a and microRNA-146b regulate human dendritic cell apoptosis and cytokine production by targeting TRAF6 and IRAK1 proteins. PMID: 25505246
    39. IL-1beta stimulation causes sequential phosphorylation of IRAK-1, c-Jun N-terminal kinase, and p300 and enhances recruitment of the p300/CBP/NF-Y complex to Gankyrin promoter PMID: 25294684
    40. The IRAK1 polymorphism is a strong independent predictor of multiple organ failure and mortality postinjury and represents a common variant with prognostic potential. PMID: 25203887
    41. Significantly lower levels of IRAK-1 was found in CAD patients with the CC genotype. PMID: 23794009
    42. miR-146a enhances the oncogenicity of oral carcinoma by concomitant targeting of the IRAK1, TRAF6 and NUMB genes. PMID: 24302991
    43. A direct interaction between IRAK1 and vasodilator-stimulated phosphoprotein (VASP) is regulated in part by assembly of IRAK1. PMID: 24857403
    44. IRAK1 rs1059702 genetic variant do not play a significant role in giant cell arteritis susceptibility or severity. PMID: 24709033
    45. Data indicate that acetyl-11-keto-beta-boswellic acid (AKBA) is able to decrease Th17 differentiation by inhibition of IL-1beta signaling via reduction of IL-1 receptor-associated kinase 1 (IRAK1) phosphorylation. PMID: 24469975
    46. Female very low birth weight infants heterozygous for the X-linked IRAK1 (rs1059703) SNP had lessgram negative bacterial infections. PMID: 23867959
    47. The data suggest the existence of two independent signals within the Xq28 region, one in IRAK1 related to pulmonary fibrosis and another in MECP2 related to diffuse cutaneous systemic sclerosis. PMID: 23444193
    48. results indicate that miR-21 is upregulated during hepatitis C virus infection and negatively regulates IFN-alpha signaling through MyD88 and IRAK1 PMID: 23633945
    49. The polymorphisms rs3027898 and rs1059702 of IRAK1 gene are associated with systemic lupus erythematosus in the Chinese Han population. PMID: 23435933
    50. Data suggest that the lupus-associated variant in the MECP2/IRAK1 locus has the potential to affect all 3 epigenetic mechanisms: DNA methylation, microRNA expression, and histone modification. PMID: 23428850

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm. Nucleus. Lipid droplet.
  • 蛋白家族：
    Protein kinase superfamily, TKL Ser/Thr protein kinase family, Pelle subfamily
  • 组织特异性：
    Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
  • 数据库链接：

    HGNC: 6112

    OMIM: 300283

    KEGG: hsa:3654

    STRING: 9606.ENSP00000358997

    UniGene: Hs.522819