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  4. Recombinant Human Hyaluronan and proteoglycan link protein 1 (HAPLN1)

Recombinant Human Hyaluronan and proteoglycan link protein 1 (HAPLN1)

  • 中文名称：
    人HAPLN1重组蛋白
  • 货号：
    CSB-MP010130HU
  • 规格：
    ￥3960
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP010130HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) HAPLN1.
    • Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP010130HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) HAPLN1.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    HAPLN1
  • Uniprot No.：
  • 别名：
    Cartilage link protein ; cartilage linking protein 1 ; Cartilage-link protein; Cartilage-linking protein 1; CLP; CRTL1 ; Crtl1l; HAPLN1; HPLN1_HUMAN; Hyaluronan and proteoglycan link protein 1; Hyaluronan and proteoglycan link protein 1 precursor ; LP; Proteoglycan link protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Mammalian cell
  • 分子量：
    41.0 kDa
  • 表达区域：
    16-354aa
  • 氨基酸序列
    DHLSDNYTLDHDRAIHIQAENGPHLLVEAEQAKVFSHRGGNVTLPCKFYRDPTAFGSGIHKIRIKWTKLTSDYLKEVDVFVSMGYHKKTYGGYQGRVFLKGGSDSDASLVITDLTLEDYGRYKCEVIEGLEDDTVVVALDLQGVVFPYFPRLGRYNLNFHEAQQACLDQDAVIASFDQLYDAWRGGLDWCNAGWLSDGSVQYPITKPREPCGGQNTVPGVRNYGFWDKDKSRYDVFCFTSNFNGRFYYLIHPTKLTYDEAVQACLNDGAQIAKVGQIFAAWKILGYDRCDAGWLADGSVRYPISRPRRRCSPTEAAVRFVGFPDKKHKLYGVYCFRAYN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    CUSABIO synthesized the recombinant gene by integrating the N-terminal 10xHis tag sequence into the targeted gene encoding the 16-354aa of the human HAPLN1. The synthesized gene was subsequently cloned into an expression vector. After cloning, the expression vector was introduced into the Mammalian cell for expression. The product was purified to obtain the recombinant human HAPLN1 protein carrying N-terminal 10xHis tag. The SDS-PAGE assayed the purity of this recombinant HAPLN1 protein greater than 85%. This HAPLN1 protein migrated along the gel to a band of about 42 kDa molecular weight.

    HAPLN1 is a gene providing instruction of making a protein named hyaluronan and proteoglycan link protein 1 (also known as proteoglycan link protein) in human and belongs to HAPLN family. The HAPLN gene family includes four members, including HAPLN1, HAPLN2, HAPLN3 and HAPLN4. Among of them, HAPLN1 is expressed in a restricted subset of adult tissues. A study has revealed that HAPLN1 loss in the aged tumor microenvironment drives a breakdown in the crosslinking of the ECM, which promotes melanoma migration while limiting intravasation of tumor-infiltrating lymphocytes.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.
  • 基因功能参考文献：
    1. HAPLN1 reflects a signaling network leading to stemness, mesenchymal commitment and hepatocellular carcinoma progression PMID: 27191501
    2. CSGalNAcT-1 and Hapln-1 may play important roles in the pathogenesis of Kashin-Beck disease and osteoarthritis. PMID: 23748413
    3. HAPLN1 is overexpressed in metastatic melanoma and is secreted exclusively by the tumor cells PMID: 22159717
    4. Single-nucleotide polymorphism in the hyaluronan and proteoglycan link protein 1 (HAPLN1) gene is associated with spinal osteophyte formation and disc degeneration in Japanese women. PMID: 20953637
    5. An in vitro model of perineuronal nets has been developed demonstrating that cartilage link protein (along with hyaluronan synthase) are necessary for their formation. PMID: 20584105
    6. link protein may function as a stabilizer of the interaction between aggrecan and hyaluronan in cartilage and between versican and hyaluronan in many tissues PMID: 14724283
    7. SOX9 is a key regulator of CRTL1 PMID: 15456769
    8. cLP and versican did not bind directly to each other in solution yet formed ternary complexes with hyaluronan PMID: 15590670
    9. Overexpression of HAPLN1 and its SP-IgV domain increases tumorigenic properties of mesothelioma. Thus, targeting the SP-IgV domain may be one of the therapeutic approaches in cancer treatment. PMID: 19351750

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    HAPLN family
  • 组织特异性：
    Widely expressed. Weakly expressed in the brain.
  • 数据库链接：

    HGNC: 2380

    OMIM: 115435

    KEGG: hsa:1404

    STRING: 9606.ENSP00000274341

    UniGene: Hs.2799