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  4. Recombinant Human Histone acetyltransferase MYST2 (KAT7), partial

Recombinant Human Histone acetyltransferase MYST2 (KAT7), partial

  • 中文名称：
    人KAT7重组蛋白
  • 货号：
    CSB-RP038644h
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP038644h could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) KAT7.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP038644h could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) KAT7.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    KAT7
  • Uniprot No.：
  • 别名：
    Hbo 1; HBO1; HBOa; Histone acetyltransferase binding to hORC1; Histone acetyltransferase binding to ORC1; Histone acetyltransferase KAT7; Histone acetyltransferase MYST2; K(lysine) acetyltransferase 7; KAT 7; KAT7; Lysine acetyltransferase 7; MOZ; MOZ YBF2/SAS3 SAS2 and TIP60 protein; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2; MYST 2; MYST histone acetyltransferase 2; MYST protein 2; MYST-2; MYST2; MYST2_HUMAN; SAS 2; SAS2 and TIP60 protein 2; TIP60 protein 2; YBF2/SAS3; ZC2HC7
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    50.8kDa
  • 表达区域：
    1-214aa
  • 氨基酸序列
    MPRRKRNAGSSSDGTEDSDFSTDLEHTDSSESDGTSRRSARVTRSSARLSQSSQDSSPVRNLQSFGTEEPAYSTRRVTRSQQQPTPVTPKKYPLRQTRSSGSETEQVVDFSDRETKNTADHDESPPRTPTGNAPSSESDIDISSPNVSHDESIAKDMSLKDSGSDLSHRPKRRRFHESYNFNMKCPTPGCNSLGHLTGKHERHFSISGCPLYHN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytic subunit of histone acetyltransferase HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), thereby regulating various processes, such as gene transcription, protein ubiquitination, immune regulation, stem cell pluripotent and self-renewal maintenance and embryonic development. Some complexes also catalyze acetylation of histone H4 at 'Lys-5', 'Lys-8' and 'Lys-12' (H4K5ac, H4K8ac and H4K12ac, respectively), regulating DNA replication initiation, regulating DNA replication initiation. Specificity of the HBO1 complexes is determined by the scaffold subunit: complexes containing BRPF scaffold (BRPF1, BRD1/BRPF2 or BRPF3) direct KAT7/HBO1 specificity towards H3K14ac, while complexes containing JADE (JADE1, JADE2 and JADE3) scaffold direct KAT7/HBO1 specificity towards histone H4. H3K14ac promotes transcriptional elongation by facilitating the processivity of RNA polymerase II. Acts as a key regulator of hematopoiesis by forming a complex with BRD1/BRPF2, directing KAT7/HBO1 specificity towards H3K14ac and promoting erythroid differentiation. H3K14ac is also required for T-cell development. KAT7/HBO1-mediated acetylation facilitates two consecutive steps, licensing and activation, in DNA replication initiation: H3K14ac facilitates the activation of replication origins, and histone H4 acetylation (H4K5ac, H4K8ac and H4K12ac) facilitates chromatin loading of MCM complexes, promoting DNA replication licensing. Acts as a positive regulator of centromeric CENPA assembly: recruited to centromeres and mediates histone acetylation, thereby preventing centromere inactivation mediated by SUV39H1, possibly by increasing histone turnover/exchange. Involved in nucleotide excision repair: phosphorylation by ATR in response to ultraviolet irradiation promotes its localization to DNA damage sites, where it mediates histone acetylation to facilitate recruitment of XPC at the damaged DNA sites. Acts as an inhibitor of NF-kappa-B independently of its histone acetyltransferase activity.; Plays a central role in the maintenance of leukemia stem cells in acute myeloid leukemia (AML). Acts by mediating acetylation of histone H3 at 'Lys-14' (H3K14ac), thereby facilitating the processivity of RNA polymerase II to maintain the high expression of key genes, such as HOXA9 and HOXA10 that help to sustain the functional properties of leukemia stem cells.
  • 基因功能参考文献：
    1. acts in concert with SNF2H-ACF1 to make the chromosome structure more accessible to canonical nucleotide excision repair factors PMID: 28719581
    2. Hbo1 is another ERalpha coactivator that ubiquitinates ERalpha PMID: 28769019
    3. Histone acetyltransferase binding to ORC1 (HBO1) is upregulated in bladder cancer. PMID: 28796367
    4. The results demonstrate some connections between KAT7 upregulated in RA SFs and RA progression and present the inhibition of KAT7 activity as a novel therapeutic target for interfering RA disease. PMID: 28552525
    5. Structural and mechanistic insights into regulation of HBO1 histone acetyltransferase activity by BRPF2 have been presented. PMID: 28334966
    6. KAT7-containing acetyltransferases associating with the Mis18 complex provides competence for histone turnover/exchange activity on alphoid DNA and prevents Suv39h1-mediated heterochromatin invasion into centromeres. PMID: 27270040
    7. the possible binding sites and biological functions of HBO1, were identified. PMID: 27247147
    8. Data show that HTLV-1 basic leucine zipper (bZIP) factor (HBZ) represses p53 activity by direct inhibition of the histone acetyltransferase (HAT) activity of p300/CBP and the HAT activity of HBO1: [HBZ] PMID: 26625199
    9. This study demonstrates that CRL4(DDB2) is a novel ubiquitin ligase of HBO1. PMID: 26572825
    10. Taken together, MYST2 is a repressed growth suppressor in AML mediating reduced acetylation of histone 4 at residue 5 and is associated with inferior AML patient survival. PMID: 26072331
    11. Myst2-mediated histone acetylation may be required for recruitment of Oct4 to the Nanog promoter PMID: 25743411
    12. Increase in the number of cells with cytoplasmic JADE1S correlated with activation of tubular cell proliferation and inversely correlated with the number of cells with nuclear JADE1S, supporting role of HBO1-JADE1 shuttling during organ regeneration. PMID: 24739512
    13. Hbo1 modulated transcription by estrogen receptor-alpha. Hbo1 contributes to degradation of ER-alpha by ubiquitination through lysine 48. PMID: 24125069
    14. Phosphorylated Hbo1 expression is associated with breast cancer. PMID: 23955388
    15. alternate choice of subunits associated with HBO1 can switch its specificity between H4 and H3 tails PMID: 24065767
    16. Gemcitabine resistance is dependent on Plk1-mediated phosphorylation of Orc2 and Hbo1. PMID: 23188630
    17. Modulation of Fbxw15 levels was able to differentially regulate histone H3K14 acetylation and cellular proliferation by altering HBO1 levels. PMID: 23319590
    18. The protein domains required for the assembly and function of HBO1/ING complexes were shown. Multiple PHD finger domains in different subunits bound the histone H3 N-terminal tail with a specificity towards lysine 4 methylation. PMID: 22144582
    19. JNK1 phosphorylation of Cdt1 inhibits recruitment of HBO1 histone acetylase and blocks replication licensing in response to stress PMID: 21856198
    20. HBO1 was an important downstream molecule of ERalpha. PMID: 21040551
    21. HBO1 promote MCM loading in vivo involves the stimulation of large-scale chromatin decondensation to allow access to the underlying DNA substrate. PMID: 20980834
    22. results show that ING4 is a bivalent reader of the chromatin H3K4me3 modification and suggest a mechanism for enhanced targeting of the HBO1 complex to specific chromatin sites PMID: 20053357
    23. H4 acetylation at origins by HBO1 is critical for DNA replication licensing. PMID: 20129055
    24. inhibition by Set/TAF-1beta inhibits active demethylation of DNA, integrating DNA methylation and transcriptional silencing PMID: 11978794
    25. ordered recruitmenet to thyroid hormone-responsive promoters in vivo PMID: 12034878
    26. Hbo1 is a positive regulatory factor for prereplicative complex assembly. PMID: 16428461
    27. histone acetyltransferase binding to ORC-1 might play an important physiological role in human progesterone receptor signaling PMID: 16645042
    28. reveal a direct regulatory connection between p53-responsive stress signaling and Hbo1-dependent chromatin pathways PMID: 17954561
    29. Hbo1 depletion results in decreased DNA replication and a failure of Mcm complex binding to chromatin, both of which can be partially rescued by the ectopic expression of WT Hbo1 but not Hbo1-S57A PMID: 18250300
    30. Jade-1/1L are crucial co-factors for HBO1-mediated histone H4 acetylation PMID: 18684714
    31. HBO1 associates with replication origins specifically during the G1 phase of the cell cycle in a manner that depends on the replication licensing factor Cdt1, but is independent of the Cdt1 repressor Geminin. PMID: 18832067
    32. Overexpression of MYST2 is associated with breast cancer. PMID: 19372580
    33. Immunohistochemistry for Hbo1 in 11 primary human tumor types revealed strong Hbo1 protein expression in carcinomas of the testis, ovary, breast, stomach/esophagus, and bladder. PMID: 19393168

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  • 亚细胞定位：
    Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytosol.
  • 蛋白家族：
    MYST (SAS/MOZ) family
  • 组织特异性：
    Ubiquitously expressed, with highest levels in testis.
  • 数据库链接：

    HGNC: 17016

    OMIM: 609880

    KEGG: hsa:11143

    STRING: 9606.ENSP00000259021

    UniGene: Hs.21907