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  4. Recombinant Human Golgi reassembly-stacking protein 1 (GORASP1)

Recombinant Human Golgi reassembly-stacking protein 1 (GORASP1)

  • 中文名称：
    人GORASP1重组蛋白
  • 货号：
    CSB-YP861116HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人GORASP1重组蛋白
  • 货号：
    CSB-EP861116HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人GORASP1重组蛋白
  • 货号：
    CSB-EP861116HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人GORASP1重组蛋白
  • 货号：
    CSB-BP861116HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人GORASP1重组蛋白
  • 货号：
    CSB-MP861116HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    FLJ23443; Golgi peripheral membrane protein p65; Golgi phosphoprotein 5; Golgi reassembly and stacking protein 1; Golgi reassembly and stacking protein 65 kDa; Golgi reassembly and stacking protein, 65-kD; Golgi reassembly stacking protein 1 65kDa; Golgi reassembly stacking protein 1; Golgi reassembly stacking protein of 65 kDa; Golgi reassembly-stacking protein 1; Golgi reassembly-stacking protein of 65 kDa; GOLPH 5; GOLPH5; Gorasp 1; GORASP1; GORS1_HUMAN; GRASP 65; GRASP65; MGC118894; MGC118897; P65
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-440
  • 氨基酸序列
    GLGVSAEQP AGGAEGFHLH GVQENSPAQQ AGLEPYFDFI ITIGHSRLNK ENDTLKALLK ANVEKPVKLE VFNMKTMRVR EVEVVPSNMW GGQGLLGASV RFCSFRRASE QVWHVLDVEP SSPAALAGLR PYTDYVVGSD QILQESEDFF TLIESHEGKP LKLMVYNSKS DSCREVTVTP NAAWGGEGSL GCGIGYGYLH RIPTQPPSYH KKPPGTPPPS ALPLGAPPPD ALPPGPTPED SPSLETGSRQ SDYMEALLQA PGSSMEDPLP GPGSPSHSAP DPDGLPHFME TPLQPPPPVQ RVMDPGFLDV SGISLLDNSN ASVWPSLPSS TELTTTAVST SGPEDICSSS SSHERGGEAT WSGSEFEVSF LDSPGAQAQA DHLPQLTLPD SLTSAASPED GLSAELLEAQ AEEEPASTEG LDTGTEAEGL DSQAQISTTE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays an important role in assembly and membrane stacking of the Golgi cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes. Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane.
  • 基因功能参考文献：
    1. In situ proximity ligation assays of Golgi localization of alpha-mannosidase IA at giantin versus GM130-GRASP65 site, and absence or presence of N-glycans terminated with alpha3-mannose on trans-Golgi glycosyltransferases may be useful for distinguishing indolent from aggressive prostate cancer cells. PMID: 28782625
    2. Results demonstrate a critical role for GRASP55 and GRASP65 in maintaining the stacked structure of the Golgi, which is required for accurate posttranslational modifications in the Golgi. Additionally, the GRASP knockout cell lines developed in this study will be useful tools for studying the role of GRASP proteins in other important cellular processes. PMID: 28814501
    3. The s determined that Golgi membrane ribbon fragmentation increased during the early cytoplasmic phase of cytomegalovirus virion assembly and that Golgi membrane fragmentation in infected cells was dependent on the phosphorylation of an integral cis-Golgi protein, Grasp65. PMID: 27703074
    4. In cells, Mena and actin were required for Golgi ribbon formation after nocodazole washout; in vitro, Mena and microfilaments enhanced GRASP65 oligomerization and Golgi membrane fusion. Thus Mena interacts with GRASP65 to promote local actin polymerization, which facilitates Golgi ribbon linking. PMID: 26538023
    5. Mutagenesis experiments support these structural observations and demonstrate that they are required for GRASP65-GM130 association. PMID: 26363069
    6. Cisternal-specific functions of GRASP65 and GRASP55 in continuity, compartmentalization, and function of the Golgi ribbon. PMID: 24227884
    7. propose that GRASP55/65 are negative regulators of exocytic transport and that this slowdown helps to ensure more complete protein glycosylation in the Golgi stack and proper sorting at the trans-Golgi network PMID: 23552074
    8. The C-terminal fragments of GRASP65 produced following caspase cleavage are targeted to mitochondria, and ectopic expression of these sensitises HeLa cells to Fas ligand. PMID: 21368855
    9. the mechanism of phosphoinhibition as direct inhibition by PLK1 of the PDZ ligand underlying the GRASP65 self-interaction. PMID: 20937827
    10. GRASP65 has a role in Golgi cisternal stacking and cell cycle progression PMID: 20214750
    11. These results demonstrate that GRASP55 and GRASP65 stack mammalian Golgi cisternae via a common mechanism. PMID: 20083603
    12. Data demonstrate that both GRASP55 and 65 are needed for the efficient transport to and through the Golgi complex, thus highlighting a novel role for the GRASPs in membrane trafficking. PMID: 19840934
    13. the GRASP domain alone of GRASP65 inhibits mitotic fragmentation of the Golgi apparatus PMID: 15576368
    14. GRASP65 may function as a signal integrator controlling the cell growth PMID: 15834132
    15. GRASP65 has a role in the regulation of spindle dynamics rather than a direct role in the stacking of Golgi cisternae PMID: 15888544
    16. Ribbon formation requires the Golgi proteins GM130 and GRASP65. PMID: 16489344
    17. Mitochondria bearing GRASP65 became tethered to one another, and this depended on a GRASP65 PDZ domain that was also required for GRASP65 self-interaction. PMID: 19581411
    18. A study showing that GRASP65 binds directly to the coiled-coil vesicle tethering factor GM130, and targets it to Golgi membranes. PMID: 9628863
    19. Identification of GRASP65, and demonstration that it functions in the formation of stacked Golgi cisternae. PMID: 9346242

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  • 亚细胞定位：
    Golgi apparatus, cis-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side. Endoplasmic reticulum-Golgi intermediate compartment membrane.
  • 蛋白家族：
    GORASP family
  • 数据库链接：

    HGNC: 16769

    OMIM: 606867

    KEGG: hsa:64689

    STRING: 9606.ENSP00000313869

    UniGene: Hs.721946