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  4. Recombinant Human General transcription factor IIH subunit 4 (GTF2H4)

Recombinant Human General transcription factor IIH subunit 4 (GTF2H4)

  • 中文名称：
    人GTF2H4重组蛋白
  • 货号：
    CSB-YP838803HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人GTF2H4重组蛋白
  • 货号：
    CSB-EP838803HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人GTF2H4重组蛋白
  • 货号：
    CSB-EP838803HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人GTF2H4重组蛋白
  • 货号：
    CSB-BP838803HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人GTF2H4重组蛋白
  • 货号：
    CSB-MP838803HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Basic transcription factor 2 52 kDa subunit; Basic transcription factor 52 kDa subunit; BTF2 p52; General transcription factor IIH polypeptide 4 52kDa; General transcription factor IIH polypeptide 4 52kDa subunit; General transcription factor IIH polypeptide 4 52kDa variant ; General transcription factor IIH polypeptide 4; General transcription factor IIH subunit 4; Gtf2h4; OTTHUMP00000029049; OTTHUMP00000164874; P52; TF2H4_HUMAN; TFB2; TFIIH basal transcription factor complex p52 subunit; TFIIH protein; TIFIIH; Transcription factor II H; Transcription factor IIH 52 kDa subunit
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-462
  • 氨基酸序列
    MESTPSRGLN RVHLQCRNLQ EFLGGLSPGV LDRLYGHPAT CLAVFRELPS LAKNWVMRML FLEQPLPQAA VALWVKKEFS KAQEESTGLL SGLRIWHTQL LPGGLQGLIL NPIFRQNLRI ALLGGGKAWS DDTSQLGPDK HARDVPSLDK YAEERWEVVL HFMVGSPSAA VSQDLAQLLS QAGLMKSTEP GEPPCITSAG FQFLLLDTPA QLWYFMLQYL QTAQSRGMDL VEILSFLFQL SFSTLGKDYS VEGMSDSLLN FLQHLREFGL VFQRKRKSRR YYPTRLAINL SSGVSGAGGT VHQPGFIVVE TNYRLYAYTE SELQIALIAL FSEMLYRFPN MVVAQVTRES VQQAIASGIT AQQIIHFLRT RAHPVMLKQT PVLPPTITDQ IRLWELERDR LRFTEGVLYN QFLSQVDFEL LLAHARELGV LVFENSAKRL MVVTPAGHSD VKRFWKRQKH SS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
  • 基因功能参考文献：
    1. functional genetic variants of GTF2H4 confer susceptibility to lung cancer. PMID: 27288692
    2. GTF2H4 variants may not be associated with susceptibility to aspirin-exacerbated respiratory disease and obstructive symptoms in asthmatics. PMID: 22524621
    3. A single nucleotide polymorphism variant within the general transcription factor IIH, polypeptide 4 gene, GTF2H4, on chromosome 6p21.33 was significantly associated with MS PMID: 20522537
    4. General transcription factor IIH protects promoters from PC4-mediated repression by relieving the topological constraint imposed by PC4 through the ERCC3 helicase activity rather than by reducing the repressive activity of PC4 via its phosphorylation. PMID: 12590132
    5. The second domain of TFIIH p62 subunit(residues 186-240) contains a region of high sequence conservation with an invariant tyrosine/phenylalanine motif which could play a key role as a protein-protein recognition module within TFIIH. PMID: 15533047
    6. TFIIH uses an expanded proximal promoter to regulate c-myc expression PMID: 15601838
    7. We conclude that the recruitment and activation of TFIIH represents a rate-limiting step for the emergence of HIV from latency. PMID: 16874302
    8. Alterations of chromatin at the RNA polymerase II stall site, which depend on CSB and TFIIH at least, are necessary for the UV-induced translocation of CSA to the nuclear matrix. PMID: 17242193
    9. TAF7 interacts with the transcription factors, TFIIH and P-TEFb, resulting in the inhibition of their Pol II CTD kinase activities PMID: 18391197
    10. TFIIH changes subunit composition in response to DNA damage. The CAK is released from the core during nucleotide excision repair (NER). PMID: 18614043
    11. For 6-4 photoproducts, we show that TFIIH complexes carrying an NH(2)-terminal XPD mutated protein are also deficient in recruitment of NER proteins downstream of TFIIH PMID: 18676829
    12. The frequency of congenital ichthyosis, collodion-baby type, was significantly higher in the TFIIH mutated group of trichothiodystrophy patients. PMID: 19681155
    13. Study demonstrates that an essential initiation factor, TFB2, forms a network of interactions with DNA near the transcription start site and facilitates promoter melting but may not be essential for promoter recognition. PMID: 19945377

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TFB2 family
  • 数据库链接：

    HGNC: 4658

    OMIM: 601760

    KEGG: hsa:2968

    STRING: 9606.ENSP00000259895

    UniGene: Hs.485070