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  4. Recombinant Human GSK3-beta interaction protein (GSKIP)

Recombinant Human GSK3-beta interaction protein (GSKIP)

  • 中文名称：
    人GSKIP重组蛋白
  • 货号：
    CSB-YP878949HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人GSKIP重组蛋白
  • 货号：
    CSB-EP878949HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人GSKIP重组蛋白
  • 货号：
    CSB-EP878949HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人GSKIP重组蛋白
  • 货号：
    CSB-BP878949HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人GSKIP重组蛋白
  • 货号：
    CSB-MP878949HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GSKIP
  • Uniprot No.：
  • 别名：
    C14ORF129; Chromosome 14 open reading frame 129 ; GSK3-beta interaction protein; GSK3beta interaction protein; GSKIP; GSKIP_HUMAN; MGC4945; UPF0279
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-139
  • 氨基酸序列
    METDCNPMEL SSMSGFEEGS ELNGFEGTDM KDMRLEAEAV VNDVLFAVNN MFVSKSLRCA DDVAYINVET KERNRYCLEL TEAGLKVVGY AFDQVDDHLQ TPYHETVYSL LDTLSPAYRE AFGNALLQRL EALKRDGQS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A-kinase anchoring protein for GSK3B and PKA that regulates or facilitates their kinase activity towards their targets. The ternary complex enhances Wnt-induced signaling by facilitating the GSK3B- and PKA-induced phosphorylation of beta-catenin leading to beta-catenin degradation and stabilization respectively. Upon cAMP activation, the ternary complex contributes to neuroprotection against oxidative stress-induced apoptosis by facilitating the PKA-induced phosphorylation of DML1 and PKA-induced inactivation of GSK3B. During neurite outgrowth promotes neuron proliferation; while increases beta-catenin-induced transcriptional activity through GSK3B kinase activity inhibition, reduces N-cadherin level to promote cell cycle progression.
  • 基因功能参考文献：
    1. Findings support the idea that in addition to its reported role in modulating Drp1, Ser637 phosphorylation causing mitochondrial elongation, GSKIP acts as a scavenger to take GSK3beta away from the beta-catenin destruction complex or as a competitor for GSK3beta binding in the Wnt pathway. PMID: 29694914
    2. findings highlight an essential compartmentalization of both PKA and GSK3beta by GSKIP, and ascribe a function to a cytosolic AKAP-PKA interaction as a regulatory factor in the control of canonical Wnt signaling. PMID: 27484798
    3. demonstrate that overexpression of ATG2B and GSKIP enhances hematopoietic progenitor differentiation, including of megakaryocytes, by increasing progenitor sensitivity to thrombopoietin PMID: 26280900
    4. analysis of the GSK3beta-GSKIPtide complex structure and the X-ray crystal structures of GSK3beta-FRATide and GSK3beta-AxinGID complexes suggests that the hydroxyl group of Y288 is crucial to maintaining a hydrogen bond network in GSK3beta-FRATide PMID: 21328310
    5. GSKIP is a naturally occurring protein that is homologous with the GSK3beta interaction domain of Axin and is able to negatively regulate GSK3beta of the Wnt signaling pathway. PMID: 16981698

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    GSKIP family
  • 组织特异性：
    Detected in heart, brain, placenta, liver, skeletal muscle, kidney, testis, lung and pancreas.
  • 数据库链接：

    HGNC: 20343

    OMIM: 616605

    KEGG: hsa:51527

    STRING: 9606.ENSP00000412315

    UniGene: Hs.4104