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  4. Recombinant Human Filamin-binding LIM protein 1 (FBLIM1)

Recombinant Human Filamin-binding LIM protein 1 (FBLIM1)

  • 货号：
    CSB-YP819890HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP819890HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP819890HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP819890HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP819890HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    FBLIM1
  • Uniprot No.：
  • 别名：
    2410043F08Rik; Cal; CSX associated LIM; DKFZp434G171; FBLI1_HUMAN; FBLIM 1; Fblim1; FBLP 1; FBLP-1; FBLP1; Filamin binding LIM protein 1; Filamin-binding LIM protein 1; Gt10; MGC105665; MIG2 interacting protein,; MIG2-interacting protein; Migfilin; Mitogen inducible 2 interacting protein; Mitogen-inducible 2-interacting protein; OTTHUMP00000003118; OTTHUMP00000003119; OTTHUMP00000003120; OTTMUSP00000011062; OTTMUSP00000011063; RP11-169K16.5; RP23-291H3.1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-373
  • 氨基酸序列
    MASKPEKRVA SSVFITLAPP RRDVAVAEEV RQAVCEARRG RPWEAPAPMK TPEAGLAGRP SPWTTPGRAA ATVPAAPMQL FNGGCPPPPP VLDGEDVLPD LDLLPPPPPP PPVLLPSEEE APAPMGASLI ADLEQLHLSP PPPPPQAPAE GPSVQPGPLR PMEEELPPPP AEPVEKGAST DICAFCHKTV SPRELAVEAM KRQYHAQCFT CRTCRRQLAG QSFYQKDGRP LCEPCYQDTL ERCGKCGEVV RDHIIRALGQ AFHPSCFTCV TCARCIGDES FALGSQNEVY CLDDFYRKFA PVCSICENPI IPRDGKDAFK IECMGRNFHE NCYRCEDCRI LLSVEPTDQG CYPLNNHLFC KPCHVKRSAA GCC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin-containing signaling complexes that control actin assembly. Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion.
  • 基因功能参考文献：
    1. data implicate FBLIM1 in the pathogenesis of sterile bone inflammation and our findings suggest CRMO is a disorder of chronic inflammation and imbalanced bone remodeling PMID: 28301468
    2. the present study emphasizes for the first time to our knowledge the role of Migfilin in osteoarthritis(OA) and highlights the importance of cell-ECM adhesion proteins in OA pathogenesis. PMID: 23237804
    3. Migfilin expression is reduced in breast cancer. PMID: 23645746
    4. alpha-parvin, beta-parvin and migfilin were expressed in tumor cells in 53%, 2%, 28% and 53% of effusions and 57%, 20%, 83% and 25% of solid lesions, respectively. PMID: 23099104
    5. Migfilin positively modulates the expression and activity of epidermal growth factor receptor, and Migfilin-mediated migration and invasion depend on epidermal growth factor receptor-induced PLC-gamma and STAT3-signaling pathways. PMID: 22843679
    6. Migfilin promoted beta-catenin degradation by reinforcing the association between beta-catenin and GSK-3beta. PMID: 22246236
    7. Migfilin can activate beta1, beta2 and beta3 integrins and promote integrin mediated responses while migfilin depletion impairs the spreading and migration of endothelial cells PMID: 22043318
    8. The association between filamin B and FBLP-1 may play a hitherto unknown role in cytoskeletal function, cell adhesion, and cell motility. PMID: 12496242
    9. A review of migfilin's role as a key regulator both at cell adhesion sites and nuclei. It's possible role in the pathogenesis of several human diseases is also discussed. PMID: 15701922
    10. Migfilin has a role in interacting with vasodilator-stimulated phosphoprotein (VASP) and regulates VASP localization to cell-matrix adhesions and migration PMID: 16531412
    11. Results suggest a role for cytoplasmic migfilin in the progression of leiomyosarcomas (LMS) and identify cytoplasmic migfilin as a potentially important biological marker for human LMS progression. PMID: 17711449
    12. Loss-of-function mutations in KIND1 result in marked variability in kindlin-1 immunolabeling in Kindler syndrome skin, which is mirrored by similar changes in kindlin-2 and migfilin immunoreactivity. PMID: 18528435
    13. analysis of the migfilin-filamin interaction and competition with integrin beta 7 tails PMID: 18829455
    14. Migfilin thus acts as a molecular switch to disconnect filamin from integrin for regulating integrin activation and dynamics of extracellular matrix-actin linkage. PMID: 19074766
    15. Migfilin interacts with Src and contributes to cell-matrix adhesion-mediated survival signaling PMID: 19833732

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  • 亚细胞定位：
    Cell junction, focal adhesion. Cytoplasm, cytoskeleton, stress fiber.
  • 组织特异性：
    Isoform 1 and isoform 3 are expressed in heart, kidney, lung, pancreas, placenta and platelets. Isoform 2 is expressed in brain, heart, kidney, lung, pancreas, placenta, skeletal muscle and platelets.
  • 数据库链接：

    HGNC: 24686

    OMIM: 607747

    KEGG: hsa:54751

    STRING: 9606.ENSP00000416387

    UniGene: Hs.530101