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  4. Recombinant Human F-box only protein 32 (FBXO32)

Recombinant Human F-box only protein 32 (FBXO32)

  • 中文名称：
    人FBXO32重组蛋白
  • 货号：
    CSB-YP839278HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人FBXO32重组蛋白
  • 货号：
    CSB-EP839278HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人FBXO32重组蛋白
  • 货号：
    CSB-EP839278HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人FBXO32重组蛋白
  • 货号：
    CSB-BP839278HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人FBXO32重组蛋白
  • 货号：
    CSB-MP839278HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    4833442G10Rik; AI430017; Atrogin 1; Atrogin-1; ATROGIN1; Atrophy gene 1; F box only protein 32; F-box only protein 32; F-box protein 32; FBX32_HUMAN; fbxo25; FBXO32; FLJ32424; MAFbx; MGC108443; MGC137646; MGC33610; Muscle atrophy F box; Muscle atrophy F box protein; Muscle atrophy F-box protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-355
  • 氨基酸序列
    MPFLGQDWRS PGQNWVKTAD GWKRFLDEKS GSFVSDLSSY CNKEVYNKEN LFNSLNYDVA AKKRKKDMLN SKTKTQYFHQ EKWIYVHKGS TKERHGYCTL GEAFNRLDFS TAILDSRRFN YVVRLLELIA KSQLTSLSGI AQKNFMNILE KVVLKVLEDQ QNIRLIRELL QTLYTSLCTL VQRVGKSVLV GNINMWVYRM ETILHWQQQL NNIQITRPAF KGLTFTDLPL CLQLNIMQRL SDGRDLVSLG QAAPDLHVLS EDRLLWKKLC QYHFSERQIR KRLILSDKGQ LDWKKMYFKL VRCYPRKEQY GDTLQLCKHC HILSWKGTDH PCTANNPESC SVSLSPQDFI NLFKF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
  • 基因功能参考文献：
    1. F-box only protein 32 (FBXO32) directly ubiquitinates C-terminal binding protein 1 (CtBP1), which is required for its stability and nuclear retention. PMID: 29142217
    2. s found that the FBXO32 and SMAD4 levels were higher in normal tissues than in CRC tissues. The expressions of FBXO32 and SMAD4 were related to clinicopathological parameters in CRC. PMID: 29465067
    3. FBXO32 activates NF-kappaB through IkappaBalpha degradation in inflammatory and genotoxic stress PMID: 28970077
    4. Transcriptional analysis of endophilin-A mutant mice, complemented by proteomics, highlighted ataxia- and protein-homeostasis-related genes and revealed upregulation of the E3-ubiquitin ligase FBXO32/atrogin-1 and its transcription factor FOXO3A. PMID: 27720640
    5. Low FBXO32 expression is associated with breast cancer tumorigenesis. PMID: 28068319
    6. Our results indicate that abnormal SCF activity with subsequent impairment of the autophagic flux due to a novel FBXO32 mutation is implicated in the pathogenesis of Dilated cardiomyopathy . PMID: 26753747
    7. Our data suggest that FBXO32 is a candidate gene for recessive familial dilated cardiomyopathy. Acting as a cardiac ubiquitin ligase, mutated FBXO32 could perturb the degradation of target proteins in the ubiquitin proteasome system. PMID: 26768247
    8. Atrogin-1 expression tended to be increased in the skeletal muscle of patients with malignant disease even before cancer related cachexia weight loss. PMID: 25760630
    9. FBXO32 targets Lys-326 of c-Myc to form polyubiquitin chains, resulting in inhibition of cell proliferation. PMID: 25944903
    10. MAFbx not only regulates protein degradation, but also reduces protein synthesis, exerting a dual role in regulating cardiac mass and preventing from cardiac hypertrophy. PMID: 24650875
    11. FBXO32 methylation status and protein expression were independently associated with survival in ESCC. FBXO32 may be a functional tumor suppressor. Its inactivation through promoter methylation could play an important role in ESCC carcinogenesis. PMID: 24798237
    12. EZH2 is a key factor in the proliferation and survival of PAX3-FOXO1 alveolar RMS cells working, at least in part, by repressing FBXO32 PMID: 24213577
    13. ATROGIN1 gene expression is increased in patients with severe burn injury. PMID: 23816995
    14. Quadriceps muscle atrogin-1 levels were lower in COPD patients than controls, but similar in patients with a low and normal fat-free mass index. Atrogin1 levels were not associated with quadriceps fiber cross-sectional area or strength in patients. PMID: 23844868
    15. Data suggest expression of atrogin-1 and MuRF-1 (muscle-specific RING finger protein 1) play role in aging-related decrease in muscle mass (i.e., sarcopenia); up-regulation of atrogin-1/MuRF-1 has potential to prevent/reverse sarcopenia. [REVIEW] PMID: 22815045
    16. Cardiac and vascular atrogin-1 mRNA expression is not associated with dexamethasone efficacy in the monocrotaline model of pulmonary hypertension. PMID: 22311109
    17. In a transgenic mouse model of autoimmune myositis, cellular inflammation is associated with FOXO3A translocation and Atrogin-1 induction. PMID: 22590725
    18. MAFbx contains two functional nuclear localization signals (NLS). Mutation or deletion of only one NLS induced cytoplasmic localization of MAFbx. PMID: 22249105
    19. investigation of factors regulating expression of two ubiquitin ligases (MAFbx and MURF1) in skeletal muscle (i.e., vastus lateralis): effects of resistance exercise and anabolic dietary supplement (i.e., branched-chain amino acids) PMID: 22127230
    20. SerpinB5 interacts with KHDRBS3 and FBXO32, and KHDRBS3 can interact with FBXO32 mRNA. PMID: 21725612
    21. atrophic AKT-FOXO signaling play major roles in eliciting pathological changes associated with diaphragm disuse. PMID: 20833824
    22. The novel tumor suppressor FBXO32 is epigenetically silenced in ovarian cancer cell lines with disrupted TGF-beta/SMAD4 signaling, and FBXO32 methylation status predicts survival in patients with ovarian cancer. PMID: 20065949
    23. Human skeletal muscle atrophy in the amyotrophic lateral sclerosis shows an increase in atrogin-1 & a decrease in Akt. The transcriptional regulation of human atrogin-1 may be controlled by an Akt-mediated transcription factor other than FKHR. PMID: 16507768
    24. results suggest that Cbl-b- or atrogin-1-mediated ubiquitination plays an important role in unloading-induced muscle atrophy, and that unloading stress may preferentially inhibit transcriptional responses in skeletal muscle PMID: 16868939
    25. Atrogin-1 mRNA expression was significantly increased in quadriceps of patients with COPD; transcriptional regulation of atrogin-1 and MuRF1 may occur via FoxO-1, but independently of AKT PMID: 17478621
    26. CSRP3, MUSTN1, SIX1, and FBXO32 expression changes in response to lengthening and shortening contractions in human muscle PMID: 17519359
    27. We conclude that smoking impairs the muscle protein synthesis process and increases the expression of genes associated with impaired muscle maintenance; smoking therefore likely increases the risk of sarcopenia. PMID: 17609255
    28. Results showed upregulation of MuRf1 and MAFbx in atrophies muscle and support their role as regulatory peptides in various conditions which lead to muscle atrophy. PMID: 17977773
    29. atrogin-1 may be a critical mediator of the muscle damage induced by statins. PMID: 17992259
    30. Testosterone represses MAFbx expression via interactions of the AR with Oct-1. PMID: 18599544
    31. Review discusses findings implicating atrogin-1, a gene required for muscle atrophy, in the pathophysiology of statin-induced muscle injury. PMID: 18681786

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  • 相关疾病：
    Defects in FBXO32 are associated with susceptibility to dilated cardiomyopathy (DCM). A disorder characterized by ventricular and impaired systolic function, resulting in heart failure and arrhythmia. Patient are at risk of premature death.
  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and the nucleus.
  • 组织特异性：
    Specifically expressed in cardiac and skeletal muscle.
  • 数据库链接：

    HGNC: 16731

    OMIM: 606604

    KEGG: hsa:114907

    STRING: 9606.ENSP00000428205

    UniGene: Hs.403933