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  4. Recombinant Human Endonuclease 8-like 2 (NEIL2)

Recombinant Human Endonuclease 8-like 2 (NEIL2)

  • 中文名称：
    人NEIL2重组蛋白
  • 货号：
    CSB-YP846576HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人NEIL2重组蛋白
  • 货号：
    CSB-EP846576HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人NEIL2重组蛋白
  • 货号：
    CSB-EP846576HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人NEIL2重组蛋白
  • 货号：
    CSB-BP846576HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人NEIL2重组蛋白
  • 货号：
    CSB-MP846576HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DNA glycosylase/AP lyase Neil2; DNA-(apurinic or apyrimidinic site) lyase Neil2; Endonuclease 8-like 2; Endonuclease VIII-like 2; NEH2; Nei endonuclease VIII like 2 (E. coli); Nei endonuclease VIII like 2; Nei homolog 2; Nei-like 2; Nei-like protein 2; NEI2; NEIL2; NEIL2_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-332
  • 氨基酸序列
    PEGPLVRKF HHLVSPFVGQ QVVKTGGSSK KLQPASLQSL WLQDTQVHGK KLFLRFDLDE EMGPPGSSPT PEPPQKEVQK EGAADPKQVG EPSGQKTLDG SSRSAELVPQ GEDDSEYLER DAPAGDAGRW LRVSFGLFGS VWVNDFSRAK KANKRGDWRD PSPRLVLHFG GGGFLAFYNC QLSWSSSPVV TPTCDILSEK FHRGQALEAL GQAQPVCYTL LDQRYFSGLG NIIKNEALYR AGIHPLSLGS VLSASRREVL VDHVVEFSTA WLQGKFQGRP QHTQVYQKEQ CPAGHQVMKE AFGPEDGLQR LTWWCPQCQP QLSEEPEQCQ FS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.
  • 基因功能参考文献：
    1. Low NEIL2 expression is associated with neoplasms. PMID: 27004405
    2. Data indicate that DNA glycosylases MYH, UNG2, MPG, NTH1, NEIL1, 2 and 3 on nascent DNA. PMID: 28575236
    3. The abnormal expressions of NEIL1, NEIL2, and NEIL3 are involved in cancer through their association with the somatic mutation load. PMID: 27042257
    4. NEIL1 and NEIL2 cooperate with TDG during base excision: TDG occupies the abasic site and is displaced by NEILs, which further process the baseless sugar, thereby stimulating TDG-substrate turnover. PMID: 26751644
    5. Studied six SNP loci: (rs2279115 of BCL2 gene, rs804270 of NEIL2 gene, rs909253 of LTA gene, rs2294008 of PSCA gene, rs3765524 and rs10509670 of PLCE1 gene) to evaluate gastric cancer risk using magnetic nanoparticles and universal tagged arrays. PMID: 26554163
    6. The variability of potentially important functional polymorphic variants of the IFNG, IFNGR2 and NEIL2 genes was characterized in representatives of four ethnic groups living in the Siberian region. PMID: 26601495
    7. variant alleles in the NEIL2 (rs804270), APE1 (rs2275008), CYP2E1 (rs2031920) and MDM2 (rs2279744) SNPs may independently influence susceptibility to gastric cancer in a Northern Jiangsu Chinese population. PMID: 26373042
    8. data strongly suggest that decreased repair of oxidative DNA base lesions due to an impaired NEIL2 expression in non-smokers exposed to SSS would lead to accumulation of mutations in genomic DNA of lung cells over time PMID: 24595271
    9. CSB protein stimulates NEIL2 DNA glycosylase activity PMID: 24406253
    10. Results suggest that the decreased DNA repair capacity of the DNA glycosylase NEIL2 R257L variant can induce mutations that lead to lung cancer development. PMID: 22497777
    11. the critical role of NEIL2 and PNKP in maintenance of the mammalian mitochondrial genome. PMID: 22130663
    12. REG4, BIRC5 and NEIL2 genes might have a role in the sensitivity of cancer patients to radiotherapy PMID: 22199273
    13. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator) PMID: 20819778
    14. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator) PMID: 20496165
    15. Observational study of gene-disease association. (HuGE Navigator) PMID: 20522537
    16. the beta2/beta3 loop where Lys50 is located in NEIL2 is important for DNA binding, presumably lies next to a phosphate-binding site, and may represent a target for regulation of the enzyme activity. PMID: 20175991
    17. NEIL2 is involved in global genome repair mainly for removing oxidative products of cytosine. PMID: 12097317
    18. NEIL1 and NEIL2 are preferentially involved in repair of lesions in DNA bubbles generated during transcription and/or replication PMID: 14522990
    19. hNEIL2 recognized an apurinic/apyrimidinic site exclusively, and the activity for 5-hydroxyuracil and guanine-derived formamidopyrimidine was marginal PMID: 14734554
    20. Reversible acetylation of Lys49 could thus regulate the repair activity of NEIL2 in vivo PMID: 15175427
    21. the zinc finger motif in NEIL2 is essential for its structural integrity and enzyme activity PMID: 15339932
    22. results show that the NEIL2-initiated repair of 5-hydroxyuracil (5-OHU) requires polynucleotide kinase (PNK) PMID: 16982218
    23. A novel missense variant C367A and several other mutations were found in familial colorectal cancer DNA suggesting a limited role for this gene in the devlopment of CRC. PMID: 17029639
    24. The damage specificity of human homologues of Endo III (hNTHl) and Endo VIII (hNEIL1 and hNEIL2) is compared to elucidate the repair role in cells. PMID: 17150535
    25. YB-1 thus appears to have a novel regulatory role in NEIL2-mediated repair under oxidative stress. PMID: 17686777
    26. NEIL2, but not NEIL1, polymorphisms may have a role in risk and progression of squamous cell carcinomas of the oral cavity and oropharynx PMID: 18594018
    27. in vivo variability in NEIL2 expression in humans identifies SNPs in the NEIL2 promoter region that have functional effects. PMID: 18651651
    28. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 18594018
    29. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator) PMID: 18515411

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    FPG family
  • 组织特异性：
    Detected in testis, skeletal muscle, heart, brain, placenta, lung, pancreas, kidney and liver.
  • 数据库链接：

    HGNC: 18956

    OMIM: 608933

    KEGG: hsa:252969

    STRING: 9606.ENSP00000284503

    UniGene: Hs.293818