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  4. Recombinant Human ERBB receptor feedback inhibitor 1 (ERRFI1)

Recombinant Human ERBB receptor feedback inhibitor 1 (ERRFI1)

  • 中文名称：
    人ERRFI1重组蛋白
  • 货号：
    CSB-YP007804HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人ERRFI1重组蛋白
  • 货号：
    CSB-EP007804HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人ERRFI1重组蛋白
  • 货号：
    CSB-EP007804HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人ERRFI1重组蛋白
  • 货号：
    CSB-BP007804HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人ERRFI1重组蛋白
  • 货号：
    CSB-MP007804HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    ERBB receptor feedback inhibitor 1; ERRFI_HUMAN; Errfi1; GENE 33; Gene 33; rat; homolog of; Mig-6; MIG6; mitogen inducible gene 6; mitogen inducible gene 6 protein; Mitogen-inducible gene 6 protein; RALT; receptor-associated late transducer
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-462
  • 氨基酸序列
    SIAGVAAQE IRVPLKTGFL HNGRAMGNMR KTYWSSRSEF KNNFLNIDPI TMAYSLNSSA QERLIPLGHA SKSAPMNGHC FAENGPSQKS SLPPLLIPPS ENLGPHEEDQ VVCGFKKLTV NGVCASTPPL TPIKNSPSLF PCAPLCERGS RPLPPLPISE ALSLDDTDCE VEFLTSSDTD FLLEDSTLSD FKYDVPGRRS FRGCGQINYA YFDTPAVSAA DLSYVSDQNG GVPDPNPPPP QTHRRLRRSH SGPAGSFNKP AIRISNCCIH RASPNSDEDK PEVPPRVPIP PRPVKPDYRR WSAEVTSSTY SDEDRPPKVP PREPLSPSNS RTPSPKSLPS YLNGVMPPTQ SFAPDPKYVS SKALQRQNSE GSASKVPCIL PIIENGKKVS STHYYLLPER PPYLDKYEKF FREAEETNGG AQIQPLPADC GISSATEKPD SKTKMDLGGH VKRKHLSYVV SP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Negative regulator of EGFR signaling in skin morphogenesis. Acts as a negative regulator for several EGFR family members, including ERBB2, ERBB3 and ERBB4. Inhibits EGFR catalytic activity by interfering with its dimerization. Inhibits autophosphorylation of EGFR, ERBB2 and ERBB4. Important for normal keratinocyte proliferation and differentiation. Plays a role in modulating the response to steroid hormones in the uterus. Required for normal response to progesterone in the uterus and for fertility. Mediates epithelial estrogen responses in the uterus by regulating ESR1 levels and activation. Important for regulation of endometrium cell proliferation. Important for normal prenatal and perinatal lung development.
  • 基因功能参考文献：
    1. MIG-6 was a direct target of miR-374a, and the expression of MIG-6 was downregulated by the overexpression of miR-374a in HepG2 cells. PMID: 28734040
    2. In Ishikawa human endometrial adenocarcinoma cell line MIG-6 negatively regulates the phosphorylation of STAT3 via direct protein interaction with STAT3. PMID: 28925396
    3. The regulation mechanism of MIG6 and suggests potential implications for the therapeutic strategies of gefitinib resistance through inhibiting MEF2C in hepatic cancer cells. PMID: 29714661
    4. Mig-6 deficiency promotes the development of Kras(G12D)-induced lung adenoma through reducing the cell apoptosis in Kras(G12D) mouse lungs partially by activating the ErbB4 pathway. PMID: 29191600
    5. Cdc42 inhibition is required for Mig-6 suppression of cell migration induced by EGF PMID: 27341132
    6. ectopic expression of Gene 33 triggers DNA damage response in an ATM serine/threonine kinase (ATM)-dependent fashion and through pathways dependent or not dependent on ABL proto-oncogene 1 non-receptor tyrosine kinase (c-Abl). PMID: 28842482
    7. our data suggest that dormant cancer cells with a high MIG6 expression level might be one of the causes of EGFR-TKI resistance in EGFR mutant lung cancer cells. PMID: 27893711
    8. Down-regulation of Mig-6 induces Cyclin D1 expression and activates the MAPK-ERK signaling pathway. Our study shows that the expression of Mig-6 protein is low in hepatocellular carcinoma, which predicts a poor prognosis. PMID: 28506767
    9. PIPKIgammai5, NEDD4-1, and Mig6 form a novel molecular nexus that controls EGFR activation and downstream signaling. PMID: 27557663
    10. Low MIG6 expression is associated with lung cancer. PMID: 26760771
    11. Upregulation of mitogen-inducible gene 6 triggers antitumor effect and attenuates progesterone resistance in endometrial carcinoma cells PMID: 26450625
    12. MIG6 down regulation may promote the migration and invasiveness of MEK inhibited mutant NRAS melanoma. PMID: 26967478
    13. MIG6 is a potent tumor suppressor for mutant EGFR-driven lung tumor initiation and progression in mice and provides a possible mechanism by which mutant EGFR can partially circumvent this tumor suppressor in human lung adenocarcinoma. PMID: 25735773
    14. Crystal structures of human EGFR-Mig6 complexes show how Mig6 rearranges after phosphorylation by EGFR to effectively irreversibly inhibit the same receptor that catalyzed its phosphorylation. PMID: 26280531
    15. MIG-6 efficiently reduces cellular transformation driven by oncogenic BRAF by orchestrating a negative feedback circuit directed towards the EGFR. PMID: 26065894
    16. study provides a new molecular mechanism to regulate EGFR signaling through modulation of MIG6 by DNAJB1 as a negative regulator. PMID: 26239118
    17. Demonstrate that Mig-6 could reverse gefitinib resistance through inhibition of EGFR/ERK pathway in non-small cell lung cancer cell lines. PMID: 25400829
    18. Mig-6 reduces pRb phosphorylation at Ser249/ Thr252 in both primary and B-Raf V600E oncogene expressing. PMID: 24815188
    19. Mig-6 is a potential biomarker for evaluation of tumor prognosis of lung cancer. PMID: 24573418
    20. Mechanistic model of EGFR endocytosis to determine the relative contributions of three parallel pathways of MIG6, ubiquitin ligase CBL and Sprouty2. PMID: 24445374
    21. The TGFbeta-miR200-MIG6 pathway orchestrates the EMT-associated kinase switch that induces resistance to EGFR inhibitors. PMID: 24830724
    22. SPRY2 and MIG6 are important regulators of wild-type and mutant EGFR trafficking and points to an EGFR expression-independent function of SPRY2 in the regulation of ERK activity that may impact cellular sensitivity to EGFR inhibitors PMID: 23868981
    23. The Mig-6 induces premature senescence via functioning in regulation of cellular senescence in normal diploid fibroblasts. PMID: 23746120
    24. The EGF receptor (EGFR) regulator MIG6 and the apoptosis regulator BIM. PMID: 24425048
    25. Data indicate that EGFR activity, which was more accurately predicted by the ratio of mitogen-inducible gene 6 (Mig6)/EGFR, highly correlated with erlotinib sensitivity in panels of cancer cell lines of different tissue origins. PMID: 23935914
    26. ERRFI1 + 808 T/G polymorphism confers protective effect on diabetic nephropathy in a Korean population. PMID: 23324575
    27. Mig6 functions as a molecular brake for beta-cell proliferation during glucocorticoid treatment in beta-cells, and thus, Mig6 may be a novel target for preventing glucocorticoid-induced impairments in functional beta-cell mass. PMID: 23384834
    28. MIG-6 gene is differentially regulated in lung cancer and melanoma and can be epigenetically silenced by inhibitors of methylation and histone deacetylation. PMID: 22701735
    29. results indicate that downregulated Mig-6 in NSCLC tissues may serve as a new marker that can predict the activation of EGFR signaling pathway PMID: 21739478
    30. These results suggest that Chk1 phosphorylates Mig-6 on Ser 251, resulting in the inhibition of Mig-6, and that Chk1 acts as a positive regulator of EGF signalling. PMID: 22505024
    31. Knockdown of PIM-1 but not of PIM-2 or PIM-3 also up regulates MIG6 expression, which identifies MIG6 as a PIM-1 regulated gene prostate cancer cells. PMID: 22193779
    32. Treatment with AZD6244 reduced the expression of mitogen-inducible gene 6 (MIG6). PMID: 22082529
    33. Mig6 plays a role in transmitting the effect of gefitinib to the downstream part of the EGFR signaling pathway. PMID: 21333004
    34. Mig-6 knockdown in thyroid cancer cell lines resulted in epidermal growth factor receptor phosphorylation and diminished NF-kappaB activity, whereas Mig-6 overexpression had the opposite effects. PMID: 21190978
    35. Mig-6 controls EGFR trafficking and suppresses gliomagenesis PMID: 20351267
    36. MIG6 drives endocytosis and degradation of kinase-inactive EGFR PMID: 20421427
    37. Mitogen-inducible gene-6 is a negative regulator of epidermal growth factor receptor signaling in hepatocytes and human hepatocellular carcinoma. PMID: 20044804
    38. Full-length Mig-6, but not CRIB domain-deleted Mig-6 (DeltaMig-6) or uncleavable mutant of Mig-6 (Mig-6-S38A), induces transcriptional activation of nuclear factor of kappaB (NFkappaB). PMID: 12384522
    39. induction by hypoxia PMID: 12387890
    40. Gene 33 is a physiological feedback inhibitor of the EGFR, functioning to inhibit EGFR phosphorylation and all events induced by EGFR activation PMID: 15556944
    41. C-terminal region of the Gene33 protein (ERBB receptor feedback inhibitor 1) regulates MEK-ERK pathway-directed Elk-dependent transcription. PMID: 15696545
    42. RALT mRNA and protein expression was strongly and selectively reduced in ERBB2-amplified breast cancer cell lines, loss of RALT signalling may adversely affect tumor responses to ErbB-2-targeting agents PMID: 15856022
    43. MIG-6 is a tumor-suppressor gene associated with lung cancer PMID: 16819504
    44. Review highlights important roles of Mig-6 in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor. PMID: 17351343
    45. The evolutionarily conserved EBR module of RALT/MIG6 mediates suppression of the EGFR catalytic activity. PMID: 17599051
    46. Crystal structures of complexes between the EGFR kinase domain and a fragment of MIG6 show that a approximately 25-residue epitope (segment 1) from MIG6 binds to the distal surface of the C lobe of the kinase domain PMID: 18046415
    47. new hypoxia-inducible and SOX9-regulated genes, Gdf10 and Chm-I. In addition, Mig6 and InhbA were induced by hypoxia, predominantly via HIF-2alpha PMID: 18077449
    48. High mitogen inducible gene 6 expression in papillary thyroid cancer is associated with greater survival and MIG-6 expression correlates directly with EGFR expression. PMID: 19040996
    49. Mig-6 is a critical regulator of the response of the endometrium to estrogen in regulating tissue homeostasis PMID: 19439667
    50. cells accumulate MIG6 as an inherent negative regulator to suppress excess EGFR activity when basal EGFR kinase activity is considerably high. PMID: 19674104

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  • 亚细胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus.
  • 蛋白家族：
    MIG6 family
  • 数据库链接：

    HGNC: 18185

    OMIM: 608069

    KEGG: hsa:54206

    STRING: 9606.ENSP00000366702

    UniGene: Hs.605445