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  4. Recombinant Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1)

Recombinant Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1)

  • 中文名称：
    人EFEMP1重组蛋白
  • 货号：
    CSB-YP007450HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人EFEMP1重组蛋白
  • 货号：
    CSB-EP007450HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人EFEMP1重组蛋白
  • 货号：
    CSB-EP007450HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人EFEMP1重组蛋白
  • 货号：
    CSB-BP007450HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人EFEMP1重组蛋白
  • 货号：
    CSB-MP007450HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    EFEMP1
  • Uniprot No.：
  • 别名：
    DHRD; Doyne honeycomb retinal degeneration; DRAD; EFEMP 1; EFEMP1; EGF containing fibulin like extracellular matrix protein 1; EGF containing fibulin like extracellular matrix protein 1 precursor ; EGF-containing fibulin-like extracellular matrix protein 1; Epidermal growth factor containing fibulin like extracellular matrix protein 1; Extracellular protein S1 5; Extracellular protein S1-5; FBLN 3; FBLN3; FBLN3_HUMAN; FBNL; FIBL 3; FIBL-3; FIBL3; Fibrillin like; Fibrillin like protein; Fibrillin-like protein; FIBULIN 3; Fibulin-3; Fibulin3; FLJ35535; Heat shock 70 KD protein 1; MGC111353; MLVT; MTLV; S1 5; T16 protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    18-493
  • 氨基酸序列
    QDT EETITYTQCT DGYEWDPVRQ QCKDIDECDI VPDACKGGMK CVNHYGGYLC LPKTAQIIVN NEQPQQETQP AEGTSGATTG VVAASSMATS GVLPGGGFVA SAAAVAGPEM QTGRNNFVIR RNPADPQRIP SNPSHRIQCA AGYEQSEHNV CQDIDECTAG THNCRADQVC INLRGSFACQ CPPGYQKRGE QCVDIDECTI PPYCHQRCVN TPGSFYCQCS PGFQLAANNY TCVDINECDA SNQCAQQCYN ILGSFICQCN QGYELSSDRL NCEDIDECRT SSYLCQYQCV NEPGKFSCMC PQGYQVVRSR TCQDINECET TNECREDEMC WNYHGGFRCY PRNPCQDPYI LTPENRCVCP VSNAMCRELP QSIVYKYMSI RSDRSVPSDI FQIQATTIYA NTINTFRIKS GNENGEFYLR QTSPVSAMLV LVKSLSGPRE HIVDLEMLTV SSIGTFRTSS VLRLTIIVGP FSF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
  • 基因功能参考文献：
    1. The extracellular matrix glycoprotein EFEMP1 is promising for future functional studies. PMID: 28374850
    2. The results demonstrate the implication of Fibulin-3 in malignant mesothelioma due to fluoro-edenite exposure. PMID: 29664355
    3. This study found a nominally different distribution of EFEMP1 genotypes, alleles and haplotypes between suicidal subjects and controls. PMID: 28327445
    4. Glioma risks were higher in carriers of single-nucleotide polymorphisms (SNPs) rs1346787 and rs3791679 in EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1) gene. PMID: 28380465
    5. MiR-338-5p suppressed proliferation, migration and invasion of glioblastoma cells through inhibiting EFEMP1. PMID: 28292024
    6. Results demonstrated that knockdown of EFEMP1 significantly inhibited ovarian cancer cell proliferation and induced cell cycle arrest at the G1/G0 phase. PMID: 27351229
    7. this review and meta-analysis shows that fibulin-3 is a useful diagnostic marker for malignant pleural mesothelioma PMID: 27769044
    8. Data suggest that HIF2alpha mediates hypoxia-induced cancer growth/metastasis and that EFEMP1 is a downstream effector of hypoxia-induced HIF2alpha during breast tumorigenesis. PMID: 27270657
    9. EFEMP1 suppresses epithelial-mesenchymal transition and migration in endometrial carcinoma PMID: 27015552
    10. Serum starvation of cultured retinal pigment epithelium cells changes the cellular distribution of cholesterol and Fibulin3 in patterns reminiscent of age-related macular degeneration. PMID: 29097185
    11. Results indicate that fibulin-3 serves as a pro-invasive factor in bladder cancer, which may be mediated through modulation of IGFBP5 expression. PMID: 28504717
    12. fibulin-3 confers a relatively high diagnostic efficacy and is acceptable to be an auxiliary biomarker to aid in malignant pleural mesothelioma identification. PMID: 28103581
    13. FBLN-3 promoter region was highly methylated in some pancreatic cancer cell lines. FBLN-3 inhibited c-MET activation and expression and reduced cellular level of ALDH1. PMID: 25451256
    14. Serum EFEMP1 is down-regulated and involved in the progression of Prostate Cancer. It may serve as a useful diagnostic biomarker, with better diagnostic accuracy than PSA in Prostate Cancer. PMID: 28085790
    15. SMRP but not FBLN3 has a role in pleural effusions in in malignant pleural mesothelioma PMID: 28314308
    16. EFEMP1 negatively modulates the migration ability in hepatocellular carcinoma. Reduction of EFEMP1 promotes the migration ability though increasing Matrix Metalloproteinase 2 and Matrix Metalloproteinase 9 expression via Extracellular Signal-Regulated MAP Kinase 1/2 activity. PMID: 27108677
    17. The EFEMP1 rs3791679 polymorphism influences glioma susceptibility in the Chinese Han population. PMID: 27706619
    18. Extensive methylation of the EFEMP1 gene promoter can discriminate invasive from benign IPMNs with superior accuracy owing to their stepwise accumulation of tumor progression PMID: 27095449
    19. The results of our study suggested an association between the EFEMP1 rs3791679 polymorphism and an elevated risk of glioma, especially in those with family history of glioma. PMID: 26823870
    20. Median FBLN-3 concentrations were 12.96. PMID: 26638921
    21. The EFEMP1 gene is significantly down-regulated in leiomyoma compared with myometrium. PMID: 26702771
    22. It was concluded that fibulin-3 is expressed at a lower level in colon cancer, and it can serve as a marker for advanced colon cancer. PMID: 26592829
    23. Novel inguinal hernia susceptibility genes are identified as EFEMP1, WT1, EBF2 and ADAMTS6. PMID: 26686553
    24. EFEMP1 might indirectly enhance the expression of MMP-2, providing a potential explanation for the role of AEG-1 in metastasis. NF-kappaB pathways might be one of the effective ways which EFEMP1 was induced by AEG-1. PMID: 25987128
    25. Suggest that FBLN-1 may play a role in arterial stiffening in patients with atherosclerosis. PMID: 26507503
    26. The polymorphisms of EFEMP1 gene were associated with breast cancer and might contribute to the susceptibility of the progression of breast cancer in Chinese Han women. PMID: 27048115
    27. Studies indicate Arg345Trp (R345W) mutation in fibulin-3 (F3) as the cause of a rare retinal dystrophy, Malattia Leventinese/Doyne Honeycomb Retinal Dystrophy (ML/DHRD). PMID: 26427406
    28. The recent finding that a common variant near EFEMP1 was associated with optic nerve-head morphology supports the possibility that the EFEMP1 variant identified in this Primary open-angle glaucoma family may be pathogenic. PMID: 26162006
    29. restoring fibulin-3 expression in breast cancer cells inhibited TGF-beta signaling, breast cancer cell EMT, invasion and metastasis in vivo. PMID: 25823021
    30. High serum Fibulin-3 levels were associated with malignant mesothelioma. PMID: 25743806
    31. EFEMP1 suppresses growth and invasion of lung cancer cells by downregulating MMP7 expression. PMID: 25676403
    32. R345W mutation in EFEMP1 is responsible for the dominant retinal drusen. PMID: 26037350
    33. The study showed that common genetic variants in EFEMP1 gene were associated with glioma and contributed to glioma susceptibility. PMID: 25638659
    34. Phenotypic assays showed that EFEMP1 de novo expression reduces malignant phenotype of PCa cells. EFEMP1 promoter methylation is prevalent in PCa and accurately discriminates PCa from non-cancerous prostate tissues and other urological neoplasms PMID: 25211630
    35. The R345W mutation in EFEMP1 caused Malattia leventinese/Doyne honeycomb retinal dystrophy in a Chinese family. PMID: 25111685
    36. R345W F3, but not WT F3, requires N-glycosylation to acquire a stable, native-like structure PMID: 25389134
    37. This study revealed that EFEMP1 SNPs could play a role in the modulation of personality traits in both healthy subjects and suicide attempters. PMID: 24241531
    38. Inhibition of ADAM10/17 or knockdown of DLL4 reduced the proangiogenic effects of fibulin-3 in culture PMID: 25139440
    39. Downregulation of fibulin-3 is associated with lung cancer invasion. PMID: 24480807
    40. Extracellular matrix proteins expression profiling in chemoresistant variants of the A2780 ovarian cancer cell line. PMID: 24804215
    41. Inhibition of gamma-secretase by RO4929097 causes at least partial sensitization of glioblastoma cells to temozolomide in vitro and in vivo. In addition, we show that EFEMP1 expression levels correlate with survival in TMZ-treated glioblastoma patients. PMID: 24495907
    42. Aberrant DNA methylation and histone H3-K9 trimethylation of EFEMP1 may be responsible for its downregulation in GC, and thus have an important role in tumor invasion and metastasis. PMID: 24718752
    43. FBLN3 suppresses both epithelial-to-mesenchymal transition and self-renewal of lung cancer stem cells by modulating the IGF1R pathway. PMID: 24013232
    44. EFEMP1 is a newly identified gene over-expressed in ovarian cancer, associated with poor clinicopathologic features and promotes angiogenesis. PMID: 24236050
    45. fibulin-3 negatively modulates the invasiveness of lung cancer cells via regulation of p38-MAPK and MMP-2/9. PMID: 24142183
    46. Subtype-independent loss of EFEMP1 expression in the majority of primary adenomas should prompt more detailed investigation in this tumor type. PMID: 24080855
    47. EFEMP1 decreased secretion of MMPs and inhibited tumor cell proliferation. PMID: 23840707
    48. Fibulin-3 exhibits antitumor effects towards hepatocellular carcinoma PMID: 23936443
    49. The top three inhibitory compounds reduced R345W fibulin-3 secretion. PMID: 23230284
    50. Define the Efemp1-p27(Kip1) axis as a potential marker module of PDAC cell sensitivity towards dual PI3K-mTOR inhibitors. PMID: 23470560

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  • 相关疾病：
    Doyne honeycomb retinal dystrophy (DHRD)
  • 亚细胞定位：
    Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Fibulin family
  • 组织特异性：
    In the eye, associated with photoreceptor outer and inner segment regions, the nerve fiber layer, outer nuclear layer and inner and outer plexiform layers of the retina.
  • 数据库链接：

    HGNC: 3218

    OMIM: 126600

    KEGG: hsa:2202

    STRING: 9606.ENSP00000347596

    UniGene: Hs.732348