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  4. Recombinant Human E3 ubiquitin-protein ligase RNF146 (RNF146)

Recombinant Human E3 ubiquitin-protein ligase RNF146 (RNF146)

  • 中文名称：
    人RNF146重组蛋白
  • 货号：
    CSB-YP889110HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RNF146重组蛋白
  • 货号：
    CSB-EP889110HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RNF146重组蛋白
  • 货号：
    CSB-EP889110HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RNF146重组蛋白
  • 货号：
    CSB-BP889110HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RNF146重组蛋白
  • 货号：
    CSB-MP889110HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RNF146
  • Uniprot No.：
  • 别名：
    Dactylidin; dJ351K20.1 ; DKFZP434O1427; E3 ubiquitin-protein ligase rnf146; RING finger protein 146; RN146_HUMAN; RNF 146; Rnf146; RP3 351K20.1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-359
  • 氨基酸序列
    MMAGCGEIDH SINMLPTNRK ANESCSNTAP SLTVPECAIC LQTCVHPVSL PCKHVFCYLC VKGASWLGKR CALCRQEIPE DFLDKPTLLS PEELKAASRG NGEYAWYYEG RNGWWQYDER TSRELEDAFS KGKKNTEMLI AGFLYVADLE NMVQYRRNEH GRRRKIKRDI IDIPKKGVAG LRLDCDANTV NLARESSADG ADSVSAQSGA SVQPLVSSVR PLTSVDGQLT SPATPSPDAS TSLEDSFAHL QLSGDNTAER SHRGEGEEDH ESPSSGRVPA PDTSIEETES DASSDSEDVS AVVAQHSLTQ QRLLVSNANQ TVPDRSDRSG TDRSVAGGGT VSVSVRSRRP DGQCTVTEV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation. Different ubiquitin linkage types have been observed: TNKS2 undergoes ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys-48'. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation. Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair.
  • 基因功能参考文献：
    1. RNF146 exerted oncogenic role through ubiquitination of Axin1 to activate beta-catenin signaling. PMID: 29932918
    2. Iduna expression under disruptive neurohomeostasis (e.g., uncompensated oxidative stress or ischemic stroke after MCAo) is upregulated by neuroprotectin D1 and thus serves as a key regulator of cell survival, halting neural cell death. PMID: 28430183
    3. the maintenance of unliganded RNF146 in an inactive state may serve to maintain the stability of the RNF146-TNKS complex, which in turn regulates the homeostasis of PARylation-dependent ubiquitination activity in the cell PMID: 25327252
    4. RNF146 regulated the development and progression of NSCLC PMID: 24454854
    5. Iduna is a Poly(ADP-ribose)-dependent ubiquitin E3 ligase. PMID: 21825151
    6. RNF146/Iduna has been found to be implicated in neurodegenerative disease and cancer development. [Review] PMID: 22274711
    7. the RNF146 WWE domain recognizes poly(ADP-ribose) (PAR) by interacting with iso-ADP-ribose (iso-ADPR), the smallest internal PAR structural unit containing the characteristic ribose-ribose glycosidic bond formed during poly(ADP-ribosyl)ation. PMID: 22267412
    8. Dactylidin may function early in the progression of Alzheimer's disease. PMID: 15813938

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  • 相关疾病：
    Defects in RNF146 are a cause of susceptibility to breast cancer.
  • 亚细胞定位：
    Cytoplasm, cytosol. Nucleus. Note=Translocates to the nucleus after DNA damage, such as laser-induced DNA breaks, and concentrates at DNA breaks. This translocation requires PARP1 activation and PAR-binding.
  • 组织特异性：
    Ubiquitously expressed. Up-regulated in brains from patients with Alzheimer disease.
  • 数据库链接：

    HGNC: 21336

    OMIM: 612137

    KEGG: hsa:81847

    STRING: 9606.ENSP00000357297

    UniGene: Hs.267120