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  4. Recombinant Human E3 ubiquitin-protein ligase CHFR (CHFR)

Recombinant Human E3 ubiquitin-protein ligase CHFR (CHFR)

  • 中文名称：
    Recombinant Human E3 ubiquitin-protein ligase CHFR(CHFR),Yeast
  • 货号：
    CSB-YP839315HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human E3 ubiquitin-protein ligase CHFR(CHFR),Yeast
  • 货号：
    CSB-EP839315HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human E3 ubiquitin-protein ligase CHFR(CHFR),Yeast
  • 货号：
    CSB-EP839315HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human E3 ubiquitin-protein ligase CHFR(CHFR),Yeast
  • 货号：
    CSB-BP839315HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human E3 ubiquitin-protein ligase CHFR(CHFR),Yeast
  • 货号：
    CSB-MP839315HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CHFR
  • Uniprot No.：
  • 别名：
    Checkpoint with forkhead and ring finger domains; Checkpoint with forkhead and RING finger domains protein; Chfr; CHFR_HUMAN; E3 ubiquitin protein ligase CHFR; E3 ubiquitin-protein ligase CHFR; EC 6.3.2.; FLJ10796; FLJ33629; RING finger protein 196; RNF 116; RNF 196; RNF116; RNF196; Ubiquitin ligase protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-664
  • 氨基酸序列
    MERPEEGKQS PPPQPWGRLL RLGAEEGEPH VLLRKREWTI GRRRGCDLSF PSNKLVSGDH CRIVVDEKSG QVTLEDTSTS GTVINKLKVV KKQTCPLQTG DVIYLVYRKN EPEHNVAYLY ESLSEKQGMT QESFEANKEN VFHGTKDTSG AGAGRGADPR VPPSSPATQV CFEEPQPSTS TSDLFPTASA SSTEPSPAGR ERSSSCGSGG GGISPKGSGP SVASDEVSSF ASALPDRKTA SFSSLEPQDQ EDLEPVKKKM RGDGDLDLNG QLLVAQPRRN AQTVHEDVRA AAGKPDKMEE TLTCIICQDL LHDCVSLQPC MHTFCAACYS GWMERSSLCP TCRCPVERIC KNHILNNLVE AYLIQHPDKS RSEEDVQSMD ARNKITQDML QPKVRRSFSD EEGSSEDLLE LSDVDSESSD ISQPYVVCRQ CPEYRRQAAQ PPHCPAPEGE PGAPQALGDA PSTSVSLTTA VQDYVCPLQG SHALCTCCFQ PMPDRRAERE QDPRVAPQQC AVCLQPFCHL YWGCTRTGCY GCLAPFCELN LGDKCLDGVL NNNSYESDIL KNYLATRGLT WKNMLTESLV ALQRGVFLLS DYRVTGDTVL CYCCGLRSFR ELTYQYRQNI PASELPVAVT SRPDCYWGRN CRTQVKAHHA MKFNHICEQT RFKN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
  • 基因功能参考文献：
    1. JNK inhibitor prevents SIRT1 phosphorylation, leading to elevated SIRT1 protein levels even in the presence of H2O2. Taken together, our results indicate that CHFR plays a crucial role in the cellular stress response pathway by controlling the stability and function of SIRT1. PMID: 27883020
    2. Re-expression of CHFR in miR-26a overexpressing cells partially rescues normal mitosis and impairs the tumorigenesis exerted by miR-26a. PMID: 28126920
    3. Data show that hypermethylation of the CHFR promoter, frequent in acute myeloid leukemia, is associated with adverse outcome, and can thus be used for risk stratification. PMID: 26542416
    4. Small molecule inhibition of CHFR-PARP1 interaction is a novel potential therapeutic approach to increase the efficacy of taxane-based chemotherapy in cancer. PMID: 26356822
    5. CHFR unmethylation is associated with oxaliplatin resistance in gastric cancer. PMID: 24748501
    6. CHFR methylation has a role in methylation of DNA damage repair and apoptotic pathway genes in non-small cell lung cancer PMID: 25828518
    7. CHFR promoter methylation is associated with colorectal cancer. PMID: 24928946
    8. CHFR-PAX5 fusion transcript is associated with B-cell precursor acute lymphoblastic leukemia. PMID: 25304615
    9. Aberrant methylation of CHFR may be associated with the pathogenesis, progression for B-cell non-Hodgkin lymphoma(B-NHL), which might be a novel molecular marker as prognosis and treatment for B-NHL. PMID: 25798877
    10. Studies indicate that checkpoint with forkhead and ring finger domains (CHFR) promoter CpG island methylation as prognostic marker. PMID: 24375389
    11. CHFR ubiquitinates and regulates TOPK levels, which is essential for its checkpoint function. PMID: 24012691
    12. CHFR methylation may have a role in chemosensitivity of paclitaxel in advanced gastric cancer PMID: 24639283
    13. CHFR methylation in CRC cell lines predicted for sensitivity in vitro and in vivo to docetaxel, while MSI-H cell lines were more sensitive to gemcitabine PMID: 23873170
    14. CHFR expression is a novel predictive marker of response and overall survival in NSCLC patients treated with taxane-containing chemotherapy PMID: 23386692
    15. CHFR aberrant methylation involves a subset of human lung adenocarcinoma associated with poor clinical outcomes. PMID: 23415374
    16. a new regulatory mechanism for CHFR that sequential post-translational modifications of CHFR by SUMO and ubiquitin coordinately regulates its stability PMID: 23454125
    17. this study underscores the importance of CHFR SUMOylation as a new regulatory mechanism of CHFR and highlights the emerging role of SUMOylation in modulating protein stability. PMID: 23131550
    18. Aggregated LDL prolongs the half life of LRP1 by preventing the receptor ubiquitinylation, at least in part, through CHFR targeting. PMID: 23241409
    19. suppression of CHFR induced a significant increase of the mitotic index and much lower numbers of cells at the G2/M phase in both cells treated with taxol, indicating mitotic checkpoint impairment PMID: 22469813
    20. CHFR is thought to contribute towards regulating mitotic entry and possible explanations for contradictory observations published on the functions and regulation of CHFR are presented. [review] PMID: 22159584
    21. The interaction between CHFR and PARP-1 plays an important role in cell cycle regulation and cancer therapeutic strategies. PMID: 22337872
    22. CHFR is epigenetically inactivated by promoter methylation in nasopharyngeal carcinoma. PMID: 22070084
    23. Hypermethylation of CHFR gene is associated with advanced gastric cancer. PMID: 21409489
    24. Aberrant methylation of the CHFR gene is a frequent event in the carcinogenesis of gastric cancer. PMID: 20109344
    25. The extent of CHFR promoter methylation correlates with RFS, indicating it is a promising epigenetic marker for recurrence. PMID: 21551253
    26. auto-ubiquitylation of E3 ubiquitin-protein ligase Chfr at G2 phase is required for accumulation of polo-like kinase 1 and mitotic entry in mammalian cell PMID: 21768102
    27. Aberrant promoter methylation of Runx3 and CHFR genes may be involved in the carcinogenesis and development of gastric cancer PMID: 20300977
    28. Hypermethylation of CHFR gene promoter is associated with loss or lower expression of CHFR mRNA in laryngeal squamous cell carcinoma. PMID: 20942233
    29. These data support a critical role for CHFR in the MAD2 spindle checkpoint. PMID: 21575600
    30. CHFR methylation in cancer tissues was significantly associated with the extent of differentiation and lymph node metastasis of gastric cancer. PMID: 21302620
    31. results demonstrate that CHFR loss might be critical for the tumorigenesis of NSCLC in patients with a history of smoking and induces tumors of a more malignant phenotype than the EGFR mutation PMID: 20473935
    32. The PBZ motif of CHFR recognizes two adenine-containing subunits of PAR and the phosphate backbone that connects them PMID: 20880844
    33. Taken together, their findings demonstrated that both epigenetic and genetic mechanisms were involved in silencing CHFR expression in EACs. PMID: 20564104
    34. The Chfr-TCTP interaction was stable throughout the cell cycle, but it could be diminished by the complete depolymerization of the microtubules. PMID: 18504434
    35. this is the first report identifying the regulatory mechanism of HLTF by CHFR, suggesting that CHFR-mediated downregulation of HLTF may help protect against cancer. PMID: 20388495
    36. Gene methylation (CHFR, E-cadherin, BNIP3) in the peritoneal fluid could detect occult neoplastic cells in the peritoneum and might be a risk factor for peritoneal metastasis. PMID: 20082478
    37. checkpoint protein Chfr is a ligase that ubiquitinates Plk1 and inhibits Cdc2 at the G2 to M transition PMID: 11807090
    38. Chfr regulates a mitotic stress pathway through its RING-finger domain with ubiquitin ligase activity. PMID: 11912157
    39. expression is downregulated by CpG island hypermethylation in esophageal cancer PMID: 12376479
    40. Frequent hypermethylation of the 5' CpG island of the mitotic stress checkpoint gene Chfr in colorectal and non-small cell lung cancer. PMID: 12538348
    41. CHFR has a role in mitotic checkpoint control PMID: 12810945
    42. Chfr may have a role in signaling the presence of mitotic stress induced by microtubule poisons PMID: 14562038
    43. That the chfr gene is frequently silenced in various tumors because of methylation of its promoter, these findings suggest that chfr is inactivated by multiple mechanisms in human cancer. PMID: 14612512
    44. Chfr is frequently inactivated in colon but not breast cancers, through a mechanism of hypermethylation of the promoter sequences. PMID: 14654793
    45. Aberrant promoter methylation of the CHFR gene was observed in a significant proportion of human gastric cancers. PMID: 15201973
    46. CHFR has a role in blocking mitosis along with p38 MAP kinase PMID: 15302856
    47. PML bodies control the distribution, dynamics and function of CHFR PMID: 15467728
    48. We conclude that the mechanism by which CHFR delays chromosome condensation involves inhibition of accumulation of Cyclin B1 in the nucleus. PMID: 15674323
    49. The loss of checkpoint control associated with methylation of CHFR suggests the potential to overcome cell cycle checkpoints, which may lead to an accumulation of mutations. PMID: 15760919
    50. downregulation of CHFR is a common event in nasopharyngeal carcinoma cells which may be due to hypermethylation of the gene promoter region PMID: 15937956

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  • 亚细胞定位：
    Nucleus, PML body.
  • 蛋白家族：
    CHFR family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 20455

    OMIM: 605209

    KEGG: hsa:55743

    STRING: 9606.ENSP00000392395

    UniGene: Hs.720197