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  4. Recombinant Human E3 ubiquitin-protein ligase ARIH1 (ARIH1)

Recombinant Human E3 ubiquitin-protein ligase ARIH1 (ARIH1)

  • 中文名称：
    Recombinant Human E3 ubiquitin-protein ligase ARIH1(ARIH1),Yeast
  • 货号：
    CSB-YP896897HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human E3 ubiquitin-protein ligase ARIH1(ARIH1),Yeast
  • 货号：
    CSB-EP896897HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human E3 ubiquitin-protein ligase ARIH1(ARIH1),Yeast
  • 货号：
    CSB-EP896897HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human E3 ubiquitin-protein ligase ARIH1(ARIH1),Yeast
  • 货号：
    CSB-BP896897HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human E3 ubiquitin-protein ligase ARIH1(ARIH1),Yeast
  • 货号：
    CSB-MP896897HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ARIH1
  • Uniprot No.：
  • 别名：
    ARI 1; ARI; ARI-1; ARI1; ARI1_HUMAN; Ariadne Drosophila homolog of; Ariadne homolog; Ariadne homolog ubiquitin conjugating enzyme E2 binding protein 1; Ariadne ubiquitin conjugating enzyme E2 binding protein homolog 1; ARIH 1; ARIH1; E3 ubiquitin protein ligase ARIH1; H7 AP2; H7-AP2; HARI; HHARI; human homologue of ariadne ; Monocyte protein 6; MOP 6; MOP-6; MOP6; Protein ariadne 1 homolog; Protein ariadne-1 homolog; Protein ariadne1 homolog; UbcH7 binding protein; UBCH7 BP; UbcH7-binding protein; UBCH7BP; UbcM4 interacting protein; UbcM4-interacting protein; Ubiquitin conjugating enzyme E2 binding protein 1; Ubiquitin-conjugating enzyme E2-binding protein 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-557
  • 氨基酸序列
    MDSDEGYNYE FDEDEECSEE DSGAEEEEDE DDDEPDDDTL DLGEVELVEP GLGVGGERDG LLCGETGGGG GSALGPGGGG GGGGGGGGGG PGHEQEEDYR YEVLTAEQIL QHMVECIREV NEVIQNPATI TRILLSHFNW DKEKLMERYF DGNLEKLFAE CHVINPSKKS RTRQMNTRSS AQDMPCQICY LNYPNSYFTG LECGHKFCMQ CWSEYLTTKI MEEGMGQTIS CPAHGCDILV DDNTVMRLIT DSKVKLKYQH LITNSFVECN RLLKWCPAPD CHHVVKVQYP DAKPVRCKCG RQFCFNCGEN WHDPVKCKWL KKWIKKCDDD SETSNWIAAN TKECPKCHVT IEKDGGCNHM VCRNQNCKAE FCWVCLGPWE PHGSAWYNCN RYNEDDAKAA RDAQERSRAA LQRYLFYCNR YMNHMQSLRF EHKLYAQVKQ KMEEMQQHNM SWIEVQFLKK AVDVLCQCRA TLMYTYVFAF YLKKNNQSII FENNQADLEN ATEVLSGYLE RDISQDSLQD IKQKVQDKYR YCESRRRVLL QHVHEGYEKD LWEYIED
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets. The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes. Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear. According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest. According to another report EIF4E2 ubiquitination leads to its subsequent degradation. Acts as the ligase involved in ISGylation of EIF4E2. In vitro, controls the degradation of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex member SUN2 and may therefore have a role in the formation and localization of the LINC complex, and as a consequence, nuclear subcellular localization and nuclear morphology.
  • 基因功能参考文献：
    1. ARIH1-mediated mitophagy promotes therapeutic resistance. PMID: 28930681
    2. Variants in ARIH1 are associated with developing aortic aneurysms. PMID: 29689197
    3. Crystal structure of HHARI and UbcH7 reveals UbcH7 approximately Ub binding RING1 domain of auto-inhibited HHARI.HHARI UBA-L domain has Ub binding properties. PMID: 28552575
    4. HHARI prevents spurious discharge of Ub from E2 to lysine residues by harboring structural elements that block E2 ~ Ub from adopting a 'closed' conformation. PMID: 28790309
    5. DNA damage induces an increase in ARIH1 protein levels and association of ARIH1 with 4EHP. In turn, this causes 4EHP recruitment to the mRNA cap, where it is known to compete with eIF4E. PMID: 25624349
    6. Determined is the three-dimensional solution structure of the catalytic RING2 domain from HHARI. It shows glimpses of a HECT E3 ligase. PMID: 24058416
    7. HHARI is a marker of cellular proliferation associated with nuclear bodies. PMID: 23059369
    8. Both parkin and HHARI bind the same substrate proteins in cells and induce the formation of aggresomes. PMID: 21590270
    9. UBCH7 exhibits activity with the RING-in-between-RING (RBR) family of E3s that includes parkin (also known as PARK2) and human homologue of ariadne (HHARI; also known as ARIH1) PMID: 21532592
    10. the structure of the RING2 from the RING-IBR-RING motif of HHARI and showed that RING2 has a completely distinct topology from classical RINGs PMID: 15236971

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Nucleus, Cajal body.
  • 蛋白家族：
    RBR family, Ariadne subfamily
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 689

    OMIM: 605624

    KEGG: hsa:25820

    STRING: 9606.ENSP00000369217

    UniGene: Hs.268787