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  4. Recombinant Human DnaJ homolog subfamily B member 11 (DNAJB11)

Recombinant Human DnaJ homolog subfamily B member 11 (DNAJB11)

  • 货号：
    CSB-YP890663HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP890663HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP890663HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP890663HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP890663HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    ABBP-2; ABBP2; APOBEC1 binding protein 2; APOBEC1-binding protein 2; DJ9; DJB11_HUMAN; DnaJ (Hsp40) homolog subfamily B member 11; DnaJ homolog subfamily B member 11; DnaJ protein homolog 9; DNAJB11; EDJ; ER associated DNAJ; ER associated dnaJ protein 3; ER associated Hsp40 co chaperone; ER-associated DNAJ; ER-associated dnaJ protein 3; ER-associated Hsp40 co-chaperone; ERdj3; ERJ3; ERj3p; hDj-9; hDj9; HEDJ; Human DnaJ protein 9; PWP1 interacting protein 4; PWP1-interacting protein 4
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    23-358
  • 氨基酸序列
    GRDFYKIL GVPRSASIKD IKKAYRKLAL QLHPDRNPDD PQAQEKFQDL GAAYEVLSDS EKRKQYDTYG EEGLKDGHQS SHGDIFSHFF GDFGFMFGGT PRQQDRNIPR GSDIIVDLEV TLEEVYAGNF VEVVRNKPVA RQAPGKRKCN CRQEMRTTQL GPGRFQMTQE VVCDECPNVK LVNEERTLEV EIEPGVRDGM EYPFIGEGEP HVDGEPGDLR FRIKVVKHPI FERRGDDLYT NVTISLVESL VGFEMDITHL DGHKVHISRD KITRPGAKLW KKGEGLPNFD NNNIKGSLII TFDVDFPKEQ LTEEAREGIK QLLKQGSVQK VYNGLQGY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    As a co-chaperone for HSPA5 it is required for proper folding, trafficking or degradation of proteins. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity. It is necessary for maturation and correct trafficking of PKD1.
  • 基因功能参考文献：
    1. DNAJB11-associated disease is a phenotypic hybrid of Autosomal-dominant polycystic kidney disease and autosomal-dominant tubulointerstitial diseases. PMID: 29706351
    2. ZAAT ER clearance resulted from enhancing ERdj3-mediated ZAAT degradation by silencing ERdj3 while simultaneously enhancing autophagy. In this context, ERdj3 suppression may eliminate the toxic gain of function associated with polymerization of ZAAT PMID: 28419579
    3. These results reveal ERdj3 tetramerization as an important structural framework for ERdj3 functions involved in coordinating endplasmic reticulum and extracellular proteostasis in the presence and absence of endplasmic reticulum stress. PMID: 28655754
    4. BiP facilitates Sec61 channel closure (i.e. limits ER Ca(2+) leakage) via the Sec61 channel with the help of ERj3 and ERj6 PMID: 26085089
    5. Depleting ERdj3 reduced the rate of mutant GCase degradation in patient-derived fibroblasts, while increasing folding, trafficking, and function by directing GCase to the profolding ER calnexin pathway. PMID: 25126989
    6. regulated co-secretion of ERdj3 with misfolded clients directly links ER and extracellular proteostasis during conditions of ER stress. PMID: 25361606
    7. ERdj3 mutant bound to unfolded endoplasmic reticulum proteins under steady state conditions in much greater amounts than wild-type. PMID: 25143379
    8. ERdjs appear to play the dual roles of increasing BiP affinity for clients and regulating delivery of clients to BiP. PMID: 23378021
    9. These data identify ERdj3 as a host protein involved with the cholera toxin intoxication process and provide new molecular details regarding cholera toxin A1-chaperone interactions. PMID: 21844235
    10. In addition, BiP formed a complex with SV40 capsids in the endoplasmic reticulum in a DNAJB11-dependent fashion. PMID: 21673190
    11. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20628086
    12. Hsp90 and Hsp40/Erdj3 were essential for K1's anti-apoptotic function. PMID: 20418907
    13. The Salmonella type III secretion effector, salmonella leucine-rich repeat protein (SlrP), targets the human chaperone ERdj3. PMID: 20335166
    14. This protein was identified as true homolog of yeast Scj1p. PMID: 15195998
    15. ERdj3 is a stress-inducible endoplasmic reticulum DnaJ homologue which serves as a cofactor for BiP's interactions with unfolded substrates PMID: 15525676
    16. These data suggest that ERdj3 might have diverse roles in the ER, including that of the molecular cochaperone of BiP and an as yet unknown protective action against vero toxin.(ERDJ3) PMID: 15544163
    17. These findings suggest that the Cys-rich region of HEDJ and its oxidation state are important in maintaining the substrate interaction domain in a binding-competent conformation. PMID: 17976514
    18. Hsp40 ERdj3 requires its Hsp70 interaction and substrate-binding properties to complement various yeast Hsp40-dependent functions PMID: 19748898

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  • 亚细胞定位：
    Endoplasmic reticulum lumen. Note=Associated with the ER membrane in a C-terminally epitope-tagged construct.
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 14889

    OMIM: 611341

    KEGG: hsa:51726

    STRING: 9606.ENSP00000265028

    UniGene: Hs.317192