Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Human Diphthamide biosynthesis protein 1 (DPH1)

Recombinant Human Diphthamide biosynthesis protein 1 (DPH1)

  • 中文名称：
    人DPH1重组蛋白
  • 货号：
    CSB-YP871625HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人DPH1重组蛋白
  • 货号：
    CSB-EP871625HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人DPH1重组蛋白
  • 货号：
    CSB-EP871625HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人DPH1重组蛋白
  • 货号：
    CSB-BP871625HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人DPH1重组蛋白
  • 货号：
    CSB-MP871625HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Candidate tumor suppressor in ovarian cancer 1; Diphthamide biosynthesis protein 1; Diphthamide biosynthesis protein 2 homolog like 1; Diphthamide biosynthesis protein 2 homolog-like 1; Diphthamide biosynthesis protein 2 like; Diphthamide biosynthesis protein 2-like; Diptheria toxin resistance protein required for diphthamide biosynthesis (Saccharomyces) like 1; Diptheria toxin resistance protein required for diphthamide biosynthesis like 1; DPH like 1 (S. cerevisiae); DPH like 1; DPH-like 1; DPH1; DPH1 homolog (S. cerevisiae); DPH1 homolog; DPH1_HUMAN; DPH2 like 1; DPH2-like 1; DPH2L; DPH2L1; FLJ33211; HsDph1; Ovarian cancer associated gene 1; Ovarian cancer associated gene 1 protein; Ovarian cancer-associated gene 1 protein; Ovarian tumor suppressor candidate 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-443
  • 氨基酸序列
    MRRQVMAALV VSGAAEQGGR DGPGRGRAPR GRVANQIPPE ILKNPQLQAA IRVLPSNYNF EIPKTIWRIQ QAQAKKVALQ MPEGLLLFAC TIVDILERFT EAEVMVMGDV TYGACCVDDF TARALGADFL VHYGHSCLIP MDTSAQDFRV LYVFVDIRID TTHLLDSLRL TFPPATALAL VSTIQFVSTL QAAAQELKAE YRVSVPQCKP LSPGEILGCT SPRLSKEVEA VVYLGDGRFH LESVMIANPN VPAYRYDPYS KVLSREHYDH QRMQAARQEA IATARSAKSW GLILGTLGRQ GSPKILEHLE SRLRALGLSF VRLLLSEIFP SKLSLLPEVD VWVQVACPRL SIDWGTAFPK PLLTPYEAAV ALRDISWQQP YPMDFYAGSS LGPWTVNHGQ DRRPHAPGRP ARGKVQEGSA RPPSAVACED CSCRDEKVAP LAP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Acts also as a tumor suppressor in lung and breast cancers. Plays a role in embryonic growth, organogenesis and postnatal survival.
  • 基因功能参考文献：
    1. novel homozygous DPH1 mutation causes intellectual disability and unique craniofacial features PMID: 29410513
    2. We used WES to identify novel compound heterozygous mutations in DPH1 (c.289delG, p.Glu97Lysfs*8 and c.491T>C, p.Leu164Pro) in a patient from a nonconsanguineous family presenting with intellectual disability, a short stature, craniofacial abnormalities, and external genital abnormalities. PMID: 29362492
    3. DPH1 functions as an oncogene in CRC and can be negatively modulated by miR-218-5p to promote CRC tumourigenesis. PMID: 29145210
    4. Results identified a second homozygous missense variant in DPH1, seen in four members of a founder population, and associated with autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies. PMID: 26220823
    5. Methylation of the DPH1 promoter causes immunotoxin resistance in acute lymphoblastic leukemia. PMID: 24070652
    6. OVCA1 could inhibit the proliferation of ovarian cancer cells by p16/cyclin D1 pathway, but not by NF-kappaB PMID: 21487939

    显示更多

    收起更多

  • 相关疾病：
    Developmental delay with short stature, dysmorphic features, and sparse hair (DEDSSH)
  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Punctate, primarily perinuclear localization.
  • 蛋白家族：
    DPH1/DPH2 family, DPH1 subfamily
  • 组织特异性：
    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, mammary gland, colon, small intestine, testis and ovary. Reduced expression in primary breast and ovarian tumors.
  • 数据库链接：

    HGNC: 3003

    OMIM: 603527

    KEGG: hsa:1801

    STRING: 9606.ENSP00000263083

    UniGene: Hs.513856