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  4. Recombinant Human Diamine acetyltransferase 1 (SAT1)

Recombinant Human Diamine acetyltransferase 1 (SAT1)

  • 中文名称：
    人SAT1重组蛋白
  • 货号：
    CSB-EP020717HU1a0
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    SAT1
  • Uniprot No.：
  • 别名：
    DC21; Diamine acetyltransferase 1; Diamine N acetyltransferase 1; EC 2.3.1.57; KFSD; KFSDX; Polyamine N acetyltransferase 1; Polyamine N-acetyltransferase 1; Putrescine acetyltransferase; SAT; SAT1; SAT1_HUMAN; Spermidine/spermine N(1) acetyltransferase 1; Spermidine/spermine N(1)-acetyltransferase 1; Spermidine/spermine N1 acetyltransferase 1; Spermidine/spermine N1 acetyltransferase alpha; SSAT 1; SSAT; SSAT-1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    23.5kDa
  • 表达区域：
    5-171aa
  • 氨基酸序列
    VIRPATAADCSDILRLIKELAKYEYMEEQVILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPEGHSIVGFAMYYFTYDPWIGKLLYLEDFFVMSDYRGFGIGSEILKNLSQVAMRCRCSSMHFLVAEWNEPSINFYKRRGASDLSSEEGWRLFKIDKEYLLKMATEE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine.
  • 基因功能参考文献：
    1. Activation of SAT1 expression induces lipid peroxidation and sensitizes cells to undergo ferroptosis upon reactive oxygen species (ROS)-induced stress, which also leads to suppression of tumor growth in xenograft tumor models. Notably, SAT1 expression is down-regulated in human tumors, and CRISPR-cas9-mediated knockout of SAT1 expression partially abrogates p53-mediated ferroptosis. Moreover, SAT1 induction is correlated PMID: 27698118
    2. our results indicated depletion of polyamines by SSAT signi fi cantly inhibited cell proliferation, migration and invasion through AKT/GSK3beta/beta-catenin signaling pathway in hepatocellular carcinoma and colorectal cancer cells. PMID: 27901475
    3. Extracellular polyamines induced proliferation and cancer cell migration by inducing ODC and SSAT expression, and the Akt1-mediated pathway. PMID: 28157137
    4. we used siRNA on SSAT and compared the SSAT level in knockdown and normal cells. Results showed that the monoclonal antibody specifically recognized SSAT. PMID: 27328064
    5. 4H6 was also compared with the commercial antibody. The produced mAbs will be a useful tool for further investigation of SSAT functions in organisms. PMID: 27228136
    6. Results show low SAT1 brain expression in depressed suicides and implicate low SAT1 brain expression in major depression independent of suicide PMID: 25959060
    7. Data suggest that SAT1 plays role in apoptosis; overexpression of SAT1 in human embryonic kidney cell line leads to a rapid depletion of spermidine and spermine, arrest in cell growth, and mitochondria-mediated apoptosis. PMID: 25849284
    8. Analysis shows a significant direct correlation between SSAT expression in Prostatic Cancer tissues and disease progression. PMID: 25893668
    9. Enhanced SSAT expression by proximal tubule epithelial cells leads to tubular damage, and its deficiency reduces the severity of renal I/R injury through reduction of cellular damage and modulation of the innate immune response PMID: 25390069
    10. A role for SAT1 in homologous recombination. PMID: 25277523
    11. The Catabolic enzyme SSAT expression levels was up-regulated in both cell lines; however, the specific SSAT siRNA treatments prevented the EBR-induced apoptosis only in LNCaP (AR+) cells. PMID: 23963538
    12. In females, the TC genotype was significantly more frequent in alcohol-dependent patients than in non-alcohol-dependent psychiatric controls. No differences were found among the males. PMID: 24735382
    13. study postulates a mechanism for SAT1 and SMOX down-regulation by post-transcriptional activity of miRNAs. PMID: 24025154
    14. SAT1 transcription is influenced by lithium and this effect is altered in bipolar disease patients who completed suicide. PMID: 23768751
    15. berberine inhibits cellular growth by affecting polyamine metabolism, in particular through the upregulation of the key catabolic enzyme, spermidine/spermine N1-acetyltransferase (SSAT). PMID: 23903781
    16. EBV-positive Akata cells demonstrated decreased SAT1 enzyme activity concomitant with altered intracellular polyamine level. PMID: 23891576
    17. SSAT translational control mechanisms PMID: 22354986
    18. SSAT induction plays a role in cell detachment and apoptosis of glioblastoma cells by N1,N11-diethylnorspermine treatment. PMID: 22179681
    19. The results of this study indicated that epigenetic factors in the promoter region of SAT1 influence gene expression levels, and may provide a mechanism for both our previous findings of haplotype-specific effects of promoter variations on SAT1 expression PMID: 21501848
    20. Studies indicate that each of the 4 genes was associated with at least one main outcome: anxiety (SAT1, SMS), mood disorders (SAT1, SMOX), and suicide attempts (SAT1, OATL1). PMID: 21152090
    21. SSAT1 may regulate exogenous gene expression by blocking steps involved in transcription/translation from an episomal vector by targeting non-polyamine substrate(s) critical for this pathway PMID: 20212040
    22. These results add support for a role of SAT1 in conferring a risk for suicide completion, in particular in the context of depressive disorders. PMID: 19851986
    23. Knockdown studies suggest that induction of SSAT and SMO is correlated with the antiproliferative effects of BENSpm with 5-FU or paclitaxel in MDA-MB-231 cells. PMID: 19727732
    24. Induction of alternatively spliced spermidine/spermine N1-acetyltransferase mRNA in the human kidney cells infected by venezuelan equine encephalitis and tick-borne encephalitis viruses PMID: 12083816
    25. overexpression of SSAT and the consequent putrescine accumulation are involved in the keratosis follicularis spinulosa decalvans phenotype PMID: 12215835
    26. characterization of promoter function in Hela cells by study of a factor, bound to the responsive element, that underwent modification by binding with another factor after X-ray irradiation PMID: 12427553
    27. genomic identification and biochemical characterization of an isoenzyme PMID: 12803540
    28. SSAT has a role in apoptosis induced by sulindac sulfone, which leads to reduced tissue polyamine contents in human colon cancer cells PMID: 14506281
    29. Transgenic SSAT-overexpressing mice are less active than syngeneic mice and show reduced aggressive behavior; furthermore, SSAT-OE mice have reduced muscle tone and grip strength, although they do not differ from syngeneic mice in several agility tasks. PMID: 15159132
    30. SSAT has a role in kidney ischemia-reperfusion injury PMID: 15213272
    31. spermidine acetyltransferase directly binds to the alpha9 cytoplasmic domain and mediates alpha9-dependent enhancement of cell migration PMID: 15479742
    32. Restoring high inducibility of SSAT activity subverts the reduced sensitivity to cisplatin of SSAT-deficient ovarian cancer cells. PMID: 15905201
    33. SSAT and SMO(PAOh1) activities are the major mediators of the cellular response of breast tumor cells to polyamines while PAO plays little or no role in this response PMID: 16207710
    34. Activation of SSAT by aspirin and different NSAIDs may be a common property of NSAIDs that plays an important role in their chemopreventive actions in colorectal cancer. PMID: 16262603
    35. tertiary structure of hSSAT reported in this article provides a sound basis for the in-depth study of its structure-function relationship PMID: 16544326
    36. The inhibition of IkappaB and activation of NFkappaB activate SSAT. PMID: 16637064
    37. a common mediator of inflammation can lead to the induction of SSAT expression by activating the NFkappaB signaling pathway in non-small cell lung cancer cells PMID: 16757480
    38. These results indicate that the disruption of polyamine homeostasis due to enhanced SSAT activity leads to DNA damage and reduced cell proliferation via activation of DNA repair and cell cycle checkpoint and disruption of Raf --> MEK --> ERK pathways. PMID: 17065202
    39. The structure of the SSAT-spermine-acetyl-coenzyme A complex suggested that Tyr140 acts as general acid and Glu92, through one or more water molecules, acts as the general base during catalysis. PMID: 17516632
    40. SSAT1, which shares 46% amino acid identity with SSAT2, also binds to HIF-1alpha and promotes its ubiquitination/degradation. However, in contrast to SSAT2, SSAT1 acts by stabilizing the interaction of HIF-1alpha with RACK1 PMID: 17875644
    41. Adenovirus-mediated expression of SSAT inhibits colorectal cancer cell growth in vitro. PMID: 18430370
    42. interaction between SLC3A2 and SAT1 suggests that these proteins may facilitate excretion of acetylated polyamines. PMID: 18660501
    43. This is the first study linking polymorphic variants of genes involved in polyamine metabolism with anxiety disorders. PMID: 18759322
    44. Our study provides new results which show that dysregulation of SSAT expression does play a role in suicide behavior. PMID: 19051286
    45. Downregulation of SAT1 expression may play a role in depression and suicidality. PMID: 19152344
    46. We failed to demonstrate a significant association between the SAT-1 single nucleotide polymorphism and schizophrenia PMID: 19162121
    47. results indicate that specific promoter variants in SAT1 have an effect on SAT1 gene expression. PMID: 19446796
    48. Adenovirus vector-mediated upregulation of spermidine /spermine N1-acetyltransferase impairs human gastric cancer growth in vitro and in vivo. PMID: 19686286

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  • 相关疾病：
    Keratosis follicularis spinulosa decalvans X-linked (KFSDX)
  • 亚细胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Acetyltransferase family
  • 数据库链接：

    HGNC: 10540

    OMIM: 308800

    KEGG: hsa:6303

    STRING: 9606.ENSP00000368572

    UniGene: Hs.28491