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  4. Recombinant Human Deleted in azoospermia protein 1 (DAZ1)

Recombinant Human Deleted in azoospermia protein 1 (DAZ1)

  • 中文名称：
    Recombinant Human Deleted in azoospermia protein 1(DAZ1),Yeast
  • 货号：
    CSB-YP868293HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human Deleted in azoospermia protein 1(DAZ1),Yeast
  • 货号：
    CSB-EP868293HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human Deleted in azoospermia protein 1(DAZ1),Yeast
  • 货号：
    CSB-EP868293HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human Deleted in azoospermia protein 1(DAZ1),Yeast
  • 货号：
    CSB-BP868293HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human Deleted in azoospermia protein 1(DAZ1),Yeast
  • 货号：
    CSB-MP868293HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DAZ1; DAZ; SPGYDeleted in azoospermia protein 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-744
  • 氨基酸序列
    MSAANPETPN STISREASTQ SSSAAASQGW VLPEGKIVPN TVFVGGIDAR MDETEIGSCF GRYGSVKEVK IITNRTGVSK GYGFVSFVND VDVQKIVGSQ IHFHGKKLKL GPAIRKQKLC ARHVQPRPLV VNPPPPPQFQ NVWRNPNTET YLQPQITPNP VTQHVQSAAN PETPNSTISR EASTQSSSAA ASQGWVLPEG KIVPNTVFVG GIDARMDETE IGSCFGRYGS VKEVKIITNR TGVSKGYGFV SFVNDVDVQK IVGSQIHFHG KKLKLGPAIR KQKLCARHVQ PRPLVVNPPP PPQFQNVWRN PNTETYLQPQ ITPNPVTQHV QSAANPETPN STISREASTQ SSSAAASQGW VLPEGKIVPN TVFVGGIDAR MDETEIGSCF GRYGSVKEVK IITNRTGVSK GYGFVSFVND VDVQKIVGSQ IHFHGKKLKL GPAIRKQKLC ARHVQPRPLV VNPPPPPQFQ NVWRNPNTET YLQPQITPNP VTQHVQAYSA YPHSPGQVIT GCQLLVYNYQ EYPTYPDSAF QVTTGYQLPV YNYQPFPAYP RSPFQVTAGY QLPVYNYQAF PAYPNSPFQV ATGYQFPVYN YQPFPAYPSS PFQVTAGYQL PVYNYQAFPA YPNSPFQVAT GYQFPVYNYQ AFPAYPNSPV QVTTGYQLPV YNYQAFPAYP SSPFQVTTGY QLPVYNYQAF PAYPNSAVQV TTGYQFHVYN YQMPPQCPVG EQRRNLWTEA YKWWYLVCLI QRRD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    RNA-binding protein that plays an essential role in spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation. Promotes germ-cell progression to meiosis and formation of haploid germ cells.
  • 基因功能参考文献：
    1. There was an increased frequency of DAZ microdeletion in blood samples from oligozoospermic and near azoospermic patients. A high frequency of DAZ microdeletion was observed in the sperm of patients with no DAZ microdeletion in their leukocytes compared to control. The results might be indicative of DAZ microdeletion induction during spermatogenesis. PMID: 28521575
    2. We investigated partial deletion of AZFc region and DAZ copy number in a population of Iranian infertile men and normozoospermic controls. PMID: 27739146
    3. Susceptibility of gr/gr rearrangements to azoospermia or oligozoospermia is dependent on DAZ and CDY1 gene copy deletions. PMID: 26149076
    4. Overexpression of DAZ1 is associated with breast tumors. PMID: 25994570
    5. Studies indicate that partial RNA-binding proteins DAZ1/2 deletion was associated wih male infertility, but partial RNA-binding proteins DAZ3/4 deletion was not associated with male infertility. PMID: 26232607
    6. We did not found any statistically significant genetic association between DAZ alleles and idiopathic male infertility PMID: 25916124
    7. genetic association study in Chinese population: Data suggest that combined patterns of copy number abnormality in DAZ1 (deleted in azoospermia 1) and/or BPY2 (basic charge Y-linked protein 2) are associated with spermatogenic impairment/azoospermia. PMID: 24935076
    8. There appears to be an asociation of DAZ1/DAZ2 deletion with spermatogenic impairment and male infertility in the South Chinese population. PMID: 23512232
    9. Report the prevalence of variations in the AZFc region of the human Y chromosome in infertile men. PMID: 23422238
    10. genetic association studies in Han population in China: Data suggest that AZF1/DAZ1 duplications underlie genetic predisposition of Y-chromosome haplogroup K* to spermatogenic impairment (azoospermia/oligospermia) in the population studied. PMID: 23696539
    11. The methylation patterns of CpG island (CGI) in the DAZ gene promoter region were different between somatic cells and spermatic cells PMID: 20170395
    12. role in male infertility - review PMID: 11688365
    13. altered transcription in azoospermia PMID: 11869379
    14. DAZ gene copy number in severely idiopathic infertile men. Gene deletion of two copies of DAZ (DAZI and 2) was the cause of spermatogenic damage. PMID: 11883873
    15. Sertoli cell function is not damaged in patients with AZFc-DAZ deletions and that the strong reduction of germ cells does not affect the FSH-inhibin B feedback loop. PMID: 12039700
    16. DAZ/DAZL protein can form a stable complex with human PUM2. PMID: 12511597
    17. Loss of only some copies of DAZ is sufficient to lead to severe male infertility, but it is not a frequent finding in cryptorchid men. PMID: 15066457
    18. Role in spermatogenesis. Decreased DAZ proteins in spermatogenic failure may be due to germ cell loss. Transcription of BOULE, DAZL, and DAZ not altered in degrees of spermatogenic failure. No increase of DAZL or BOULE found in DAZ deletion. PMID: 15066460
    19. The deleted in azoospermia (DAZ) are believed to have important function in sperm production, since DAZ is frequently deleted in azoospermic and severy oligozoospermic men. PMID: 15253135
    20. The frequency of partial copy deletion of DAZ gene in Chinese idiopathic azoospermia or severe oligozoospermia patients is much higher, suggesting that the deletion of DAZ1/DAZ2 may be one of the important factors of spermatogenesis damage. PMID: 15476166
    21. DAZ activates translationally silent mRNAs during germ cell development through the direct recruitment of polyA-binding proteins. PMID: 16001084
    22. Additional polymorphisms identified within the DAZ repeat regions of the DAZ genes indicate that sister chromatid exchange plays a significant role in the genesis of deletions, duplications, and polymorphisms of the Y chromosome. PMID: 16085382
    23. DAZ genes are prone to deletions and duplications. Partial DAZ gene deletions are associated with oligozoospermia. PMID: 16275261
    24. An association between DAZ haplotypes and Y chromosome haplogroups was found, and data show that the DAZ gene is not under selective constraints and its evolution depends only on the mutation rate. PMID: 16777954
    25. The results showed that DAZ gene activity seems to correspond to the proliferative activity of stem cells of germinal epithelium. PMID: 16805138
    26. DAZ cannot bind simultaneously to DAZAP1 and poly(A)-binding protein (PABP), and suggest that the phosphorylation-induced dissociation of DAZ and DAZAP1 may allow the former to stimulate translation by interacting with PABP. PMID: 16848763
    27. complete DAZ deletion is a frequent genetic cause of severe oligozoospermia, and DAZ1/DAZ2 deletion is a high risk factor for the disease PMID: 16963411
    28. AZFc subdeletions do not seem to cause severe impairment of spermatogenesis in Chilean men. PMID: 17416365
    29. Quantitative real-time PCR assays of this protein gave positive predictive values of 78 per cent for the recovery of sperm from testicular biopsy. PMID: 17453684
    30. The data show no relationship between 'gr/gr' AZC gene deletion and cryptorchidism. PMID: 17609244
    31. Patients with complete Sertoli cell-only syndrome (SCOS) did not exhibit DAZ gene expression. PMID: 17881721
    32. The expression of DAZ proteins in adult human testes is restricted to the spermatogonia and suggests a premeiotic role. PMID: 18385127
    33. AZFc rearrangements/polymorphisms are transmitted to sons and may represent a risk factor for decreased testis function and male subfertility, which needs confirmation in further studies in larger cohorts PMID: 18440997
    34. deletions of two DAZ gene copies are compatible with normal spermatogenesis and fertility. PMID: 18440997
    35. PCR and FISH demonstrated tandem duplication/multiplication of the SRY and DAZ genes in the two Turner Syndrome patients having intact Y chromosome in >85% cells PMID: 19030103
    36. All four DAZ genes are expressed in the human testis, and their products are highly polymorphic among men PMID: 19223287
    37. human DAZL (deleted in azoospermia-like) functions in primordial germ-cell formation, whereas closely related genes DAZ and BOULE (also called BOLL) promote later stages of meiosis and development of haploid gametes PMID: 19865085

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  • 相关疾病：
    Spermatogenic failure Y-linked 2 (SPGFY2)
  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Nuclear at some stages of spermatozoide development. Localizes both to the nuclei and cytoplasm of spermatozoide differentiation. Nuclear in fetal gonocytes and in spermatogonial nuclei. It then relocates to the cytoplasm during male meiosis.
  • 蛋白家族：
    RRM DAZ family
  • 组织特异性：
    Testis-specific. Expression restricted to premeiotic germ cells, particularly in spermatogonia (at protein level).
  • 数据库链接：

    HGNC: 2682

    OMIM: 400003

    KEGG: hsa:1617

    STRING: 9606.ENSP00000384573

    UniGene: Hs.522868