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  4. Recombinant Human DNA repair protein RAD51 homolog 2 (RAD51B)

Recombinant Human DNA repair protein RAD51 homolog 2 (RAD51B)

  • 中文名称：
    人RAD51B重组蛋白
  • 货号：
    CSB-YP019267HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RAD51B重组蛋白
  • 货号：
    CSB-EP019267HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RAD51B重组蛋白
  • 货号：
    CSB-EP019267HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RAD51B重组蛋白
  • 货号：
    CSB-BP019267HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RAD51B重组蛋白
  • 货号：
    CSB-MP019267HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DNA repair protein RAD51 homolog 2; hREC2; MGC34245; OTTHUMP00000212251; OTTHUMP00000212253; OTTHUMP00000212254; OTTHUMP00000212255; R51H2; RA51B_HUMAN; RAD51 homolog B (S. cerevisiae); RAD51 homolog B; RAD51 like 1; RAD51 like protein 1; RAD51-like protein 1; Rad51B; RAD51L1; REC2; RecA like protein; Recombination repair protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-384
  • 氨基酸序列
    MGSKKLKRVG LSQELCDRLS RHQILTCQDF LCLSPLELMK VTGLSYRGVH ELLCMVSRAC APKMQTAYGI KAQRSADFSP AFLSTTLSAL DEALHGGVAC GSLTEITGPP GCGKTQFCIM MSILATLPTN MGGLEGAVVY IDTESAFSAE RLVEIAESRF PRYFNTEEKL LLTSSKVHLY RELTCDEVLQ RIESLEEEII SKGIKLVILD SVASVVRKEF DAQLQGNLKE RNKFLAREAS SLKYLAEEFS IPVILTNQIT THLSGALASQ ADLVSPADDL SLSEGTSGSS CVIAALGNTW SHSVNTRLIL QYLDSERRQI LIAKSPLAPF TSFVYTIKEE GLVLQETTFC SVTQAELNWA PEILPPQPPE QLGLQMCHHT QLIF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway.
  • 基因功能参考文献：
    1. We successfully identified a common variant, rs911263, as being significantly associated with the disease status . In addition, this SNP was shown to be related to erosion, a clinical assessment of disease severity in RA (P = 2.89 x 10(-5), OR = 0.52). These findings shed light on the role of RAD51B in the onset and severity of Rheumatoid arthritis (RA). PMID: 28361912
    2. hypermethylation of homologous recombination DNA repair genes including RAD51B and XRCC3 is associated with an inflamed phenotype in squamous cell cancers of the head and neck, lung and cervix. PMID: 27683114
    3. our study suggested that miRNA-binding site genetic variants of RAD51B may modify the susceptibility to cervical cancer, which is important to identify individuals with differential risk for this malignancy and to improve the effectiveness of preventive intervention. PMID: 27334422
    4. common variation is significantly associated with familial breast cancer risk PMID: 27149063
    5. over-expression of RAD51B promoted cell proliferation, aneuploidy, and drug resistance, while RAD51B knockdown led to G1 arrest and sensitized cells to 5-fluorouracil PMID: 27651161
    6. a novel germ line RAD51B nonsense mutation, and reduced expression of RAD51B in melanoma cells indicating inactivation of RAD51B PMID: 25600502
    7. Mutations in epithelial ovarian cancer cases were more frequent in RAD51C (14 occurrences, 0.41%) and RAD51D (12 occurrences, 0.35%) than in RAD51B (two occurrences, 0.06%). PMID: 26261251
    8. the risk of developing AMD exhibits dose dependency as well as an epistatic combined effect in rs17105278 T>C and rs4902566 C>T carriers and that the elevated risk for rs17105278 T>C carriers may be due to decreased transcription of RAD51B. PMID: 24526414
    9. Relative excess risk of breast cancer due to interaction between RAD51L1 single-nucleotide polymorphism and BMI. PMID: 25255808
    10. Data indicate that complement factor H (CFH) R1210C and common variants in COL8A1 and RAD51B plus six genes contribute predictive information for advanced macular degeneration (AMD) beyond macular and behavioral phenotypes. PMID: 24498017
    11. It confirms that RAD51 paralog mutations confer breast and ovarian cancer predisposition and are rare events. PMID: 24139550
    12. This study provides robust evidence for an association of rheumatoid arthritis susceptibility with genes involved in B cell differentiation (BACH2) and DNA repair (RAD51B). PMID: 24022229
    13. SNP in RAD51B at 14q24.1 was significantly associated with male breast cancer risk PMID: 23001122
    14. Single nucleotide polymorphism in RAD51L1 is associated with breast cancer. PMID: 22454379
    15. our results suggest that RAD51L1 is unlikely to represent a high-penetrance breast cancer susceptibility gene. PMID: 21533530
    16. Single Nucleotide Polymorphisms in RAD51L1 gene is associated with glioblastoma. PMID: 22017238
    17. rs11249433 at 1p.11.2, and two highly correlated single-nucleotide polymorphisms rs999737 and rs10483813 (r(2)= 0.98) at 14q24.1 (RAD51L1), for up to 46 036 invasive breast cancer cases and 46 930 controls from 39 studies, were genotyped. PMID: 21852249
    18. findings support the notion that DNA repair genes, in particular RAD51L1, play a role in nasopharyngeal carcinoma etiology and development PMID: 21368091
    19. polymorphisms in and haplotypes of the RAD51L1 gene, which is involved in the double-strand break repair pathway, modulate gamma-radiation-induced mutagen sensitivity. PMID: 20610542
    20. BCR/ABL fragments were used for identifying the sites of BCR/ABL interaction with RAD51B PMID: 19657362
    21. involved in the frequently occurring t(6;14) (p21;q23-->q24) in pulmonary chondroid hamartomas PMID: 11978964
    22. Rad51B protein may have a specific function in Holliday junction processing in the homologous recombinational repair pathway in humans PMID: 12441335
    23. motif in the N-terminus of Rad51B serves as an NLS that allows Rad51B to localize to the nucleus independent of Rad51C or BRCA2 PMID: 15701685
    24. A multistage genome-wide association study in breast cancer identifies two new risk alleles at 1p11.2 and 14q24.1 (RAD51L1). PMID: 19330030

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  • 相关疾病：
    A chromosomal aberration involving RAD51B is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with HMGA1.
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    RecA family, RAD51 subfamily
  • 组织特异性：
    Expressed in a wide range of tissues.
  • 数据库链接：

    HGNC: 9822

    OMIM: 150699

    KEGG: hsa:5890

    STRING: 9606.ENSP00000419471

    UniGene: Hs.172587