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  4. Recombinant Human DNA/RNA-binding protein KIN17 (KIN)

Recombinant Human DNA/RNA-binding protein KIN17 (KIN)

  • 中文名称：
    人KIN重组蛋白
  • 货号：
    CSB-YP012351HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人KIN重组蛋白
  • 货号：
    CSB-EP012351HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人KIN重组蛋白
  • 货号：
    CSB-EP012351HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人KIN重组蛋白
  • 货号：
    CSB-BP012351HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人KIN重组蛋白
  • 货号：
    CSB-MP012351HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    KIN
  • Uniprot No.：
  • 别名：
    KIN; BTCD; KIN17DNA/RNA-binding protein KIN17; Binding to curved DNA; KIN; antigenic determinant of recA protein homolog
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-393
  • 氨基酸序列
    MGKSDFLTPK AIANRIKSKG LQKLRWYCQM CQKQCRDENG FKCHCMSESH QRQLLLASEN PQQFMDYFSE EFRNDFLELL RRRFGTKRVH NNIVYNEYIS HREHIHMNAT QWETLTDFTK WLGREGLCKV DETPKGWYIQ YIDRDPETIR RQLELEKKKK QDLDDEEKTA KFIEEQVRRG LEGKEQEVPT FTELSRENDE EKVTFNLSKG ACSSSGATSS KSSTLGPSAL KTIGSSASVK RKESSQSSTQ SKEKKKKKSA LDEIMEIEEE KKRTARTDYW LQPEIIVKII TKKLGEKYHK KKAIVKEVID KYTAVVKMID SGDKLKLDQT HLETVIPAPG KRILVLNGGY RGNEGTLESI NEKTFSATIV IETGPLKGRR VEGIQYEDIS KLA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo. Binds via its C-terminal domain to RNA in vitro.
  • 基因功能参考文献：
    1. Findings demonstrate that kin17 is closely related to the cell proliferation and invasion of cervical cancer. PMID: 28346239
    2. overexpression of KIN rendered colorectal cancer cells enriching cancer stem cell (CSC) markers and CSC phenotype, and silencing KIN reduced CSC markers and CSC phenotype. PMID: 24755081
    3. Trimethylated at Lys-135 by METTL22 PMID: 23349634
    4. up-regulation of kin17 is strongly associated with cellular proliferation, DNA replication, DNA damage response and breast cancer development PMID: 21980430
    5. ionizing radiation triggers chromatin complex formation in human cells PMID: 11880372
    6. The overexpression of human kin17 protein in vivo and the introduction of increased amounts of human kin17 protein in an in vitro assay reduced T-antigen-dependent DNA replication. PMID: 12359749
    7. results indicate the presence of functional XPA and XPC proteins is essential for the up-regulation of the KIN17 gene after UVC irradiation; also show that the integrity of global genome repair is required to trigger KIN17 gene expression PMID: 12525703
    8. KIN17 protein may be a component of the DNA replication machinery that participates in the cellular response to unrepaired DSBs, and an impaired KIN17 pathway leads to an increased sensitivity to ionizing radiation. PMID: 12751957
    9. A chromatin-associated protein conserved during evolution and overproduced in certain human tumor cell lines, which is involved in DNA replication. PMID: 12754299
    10. Kin17 protein strongly associates in vivo with DNA fragments containing replication origins in both human HeLa and monkey CV-1 cells. PMID: 15831485
    11. C-terminal domain of human KIN17 has a basic pI, heavy-atom derivatives were obtained by soaking crystals with negatively charged ions such as tungstate & iodine. Replacement of LiCl by KI in cryosolution allowed determination of phases from iodide ions. PMID: 16511313
    12. Analysis of KIN17 structure complexed with tungstate shows structural variability within the domain PMID: 17045609
    13. Thus, human KIN17 region 51-160 might rather be involved in protein-protein interaction through its conserved surface centered on the 3(10)-helix. PMID: 18029424

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    KIN17 family
  • 组织特异性：
    Ubiquitously expressed in all tissues examined, with highest levels in skeletal muscle, heart and testis. Differentially expressed in non-tumorigenic and tumorigenic cell lines. Highly expressed in proliferating epithelial keratinocyte cells in vitro (at
  • 数据库链接：

    HGNC: 6327

    OMIM: 601720

    KEGG: hsa:22944

    STRING: 9606.ENSP00000368881

    UniGene: Hs.397918