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  4. Recombinant Human DEP domain-containing mTOR-interacting protein (DEPTOR)

Recombinant Human DEP domain-containing mTOR-interacting protein (DEPTOR)

  • 中文名称：
    Recombinant Human DEP domain-containing mTOR-interacting protein(DEPTOR),Yeast
  • 货号：
    CSB-YP851530HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human DEP domain-containing mTOR-interacting protein(DEPTOR),Yeast
  • 货号：
    CSB-EP851530HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human DEP domain-containing mTOR-interacting protein(DEPTOR),Yeast
  • 货号：
    CSB-EP851530HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human DEP domain-containing mTOR-interacting protein(DEPTOR),Yeast
  • 货号：
    CSB-BP851530HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human DEP domain-containing mTOR-interacting protein(DEPTOR),Yeast
  • 货号：
    CSB-MP851530HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DEP domain containing 6; DEP domain-containing mTOR-interacting protein; DEP domain-containing protein 6; DEP.6; DEPDC6; DEPTOR; DPTOR_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-409
  • 氨基酸序列
    MEEGGSTGSA GSDSSTSGSG GAQQRELERM AEVLVTGEQL RLRLHEEKVI KDRRHHLKTY PNCFVAKELI DWLIEHKEAS DRETAIKLMQ KLADRGIIHH VCDEHKEFKD VKLFYRFRKD DGTFPLDNEV KAFMRGQRLY EKLMSPENTL LQPREEEGVK YERTFMASEF LDWLVQEGEA TTRKEAEQLC HRLMEHGIIQ HVSNKHPFVD SNLLYQFRMN FRRRRRLMEL LNEKSPSSQE THDSPFCLRK QSHDNRKSTS FMSVSPSKEI KIVSAVRRSS MSSCGSSGYF SSSPTLSSSP PVLCNPKSVL KRPVTSEELL TPGAPYARKT FTIVGDAVGW GFVVRGSKPC HIQAVDPSGP AAAAGMKVCQ FVVSVNGLNV LHVDYRTVSN LILTGPRTIV MEVMEELEC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.
  • 基因功能参考文献：
    1. key modulator that plays important roles in fine tuning the activity of mTOR and the activation state of the PI3K-AKT axis, with a deep impact on cancer, metabolism, and immunity [review] PMID: 29897294
    2. Using computational modeling, systems analysis and dynamic simulations, study shows that DEPTOR confers remarkably rich and complex dynamic behaviours to mTOR signalling, including abrupt, bistable switches, oscillations and co-existing bistable/oscillatory responses. Transitions between these distinct modes of behavior are enabled by modulating DEPTOR expression alone. PMID: 29330362
    3. In ASS1-knockout cells, DEPTOR, an inhibitor of mTORC1 signal, was downregulated and mTORC1 signaling was more activated in response to arginine. PMID: 28358054
    4. Deptor depletion induces endoplasmic reticulum (ER) stress and synergizes the effect of the proteasome inhibitor bortezomib (Bz) in multiple myeloma (MM) cells. PMID: 27655709
    5. DEPTOR overexpression is associated with cervical squamous cell carcinoma. PMID: 26992219
    6. Studies provide evidence that Deptor appears to play an important role in the pathogenesis of many types of cancers, mainly through its role in controlling the activity of mTOR and many other possible functions. [review] PMID: 28086984
    7. Low DEPTOR expression is associated with esophageal squamous cell carcinoma. PMID: 26893358
    8. Association between insulin sensitivity and genetic variants in DEPTOR gene suggest DEPTOR and mammalian target of rapamycin signaling pathway to be potential target for future research and pharmacological interventions. PMID: 26871578
    9. High DEPTOR expression is associated with T-cell leukemia. PMID: 27593934
    10. Results provide evidence that DEPTOR acts as a tumor suppressor by limiting EGFR-driven lung adenocarcinoma progression. PMID: 26896556
    11. that DEPTOR expression is required to maintain myeloma cell differentiation and high level of its expression are associated with better outcome. PMID: 28420429
    12. High expression of DEPTOR benefits esophageal squamous cell carcinoma patients in early stage but not advanced stage. PMID: 28267437
    13. the present findings supported the fact that DEPTOR-mTOR signaling is a central regulator of lipid metabolism-mediated inflammation in lymphocytes of PBMC culture. PMID: 28349073
    14. Cul1 promoted mTORC1 activity and cap-dependent translation by enhancing the ubiquitination and degradation of DEPTOR. PMID: 26717892
    15. Deptor enhances triple-negative breast cancer metastasis and chemoresistance through coupling to survivin expression PMID: 25810016
    16. Data suggest that DEPTOR has a tumor suppressive activity against pancreatic cancer cells, and its loss of expression may contribute to pancreatic tumorigenesis. PMID: 25544749
    17. Data indicate that phosphatidic acid (PA) specifically disrupts DEPTOR-mTORC1 interaction. PMID: 25936805
    18. conclude that DEPTOR-related mTOR suppression is involved in metformin's anti-cancer action in liver, and could be a novel target for anti-cancer therapy PMID: 25843797
    19. our data provide evidence for the requirement of TGFbeta-activated mTORC1 only by deptor downregulation, which dominates upon the bystander mTORC2 activity for enhanced expression of collagen I (alpha2). PMID: 25333702
    20. In Alzheimer's disease patients the mTOR pathway can be overactive, leading to a dysfunction of autophagy and a lack of amyloid beta clearance so the excess of amyloid beta will cause a further decrease in DEPTOR and an increase in mTOR expression. PMID: 25119265
    21. Receptor CXCR4 signaling promotes lysosomal and ESCRT-dependent degradation of DEPTOR, an antagonist of mTORC2, that regulates Akt activation and signaling. PMID: 25605718
    22. Downregulation of DEPTOR (p=0.331) in CRC. PMID: 24969890
    23. DEPTOR plays a key role in maintaining stem cell pluripotency by limiting mTOR activity in undifferentiated embryonic stem cells PMID: 25258312
    24. Raf-1/JNK /p53/p21 pathway may be involved in apoptosis, and NFkappaB1 may play a possible role in inhibiting apoptosis. PMID: 22282237
    25. Downregulation of DEPTOR inhibited proliferation and increased chemosensitivity to melphalan in human multiple myeloma RPMI-8226 cells via inhibiting the PI3K/AKT pathway. PMID: 23613505
    26. these findings define DEPTOR as a critical upstream regulator of endothelial cell activation responses. PMID: 23881914
    27. the downregulation of DEPTOR induces apoptosis, increases chemosensitivity to doxorubicin, and suppresses autophagy and the activation of the PI3K/Akt signaling pathway in RPMI8226 human multiple myeloma cells. PMID: 23503641
    28. Deptor is induced by the Baf60c-Six4 transcriptional complex and mediates activation of Akt and glycolytic metabolism by Baf60c in a cell-autonomous manner PMID: 23563706
    29. this review discusses beta-TrCP's new downstream substrate, DEPTOR, as well as summarize the novel functional aspects of beta-TrCP in controlling cell growth and regulating autophagy, in part through governing the stability of DEPTOR.[Review] PMID: 22454292
    30. GNMT regulates hepatocellular carcinoma growth in part through interacting with DEPDC6/DEPTOR and modulating mTOR/raptor signaling pathway PMID: 22160218
    31. These novel findings are indicative of a higher order of complexity of DEPTOR signalling in the human placenta that is affected by maternal stress. PMID: 21992080
    32. misregulation of the DEPTOR destruction pathway might contribute to aberrant activation of mTOR in disease. PMID: 22017875
    33. DEPTOR, an mTOR inhibitor, is a physiological substrate of SCF(betaTrCP) E3 ubiquitin ligase and regulates survival and autophagy PMID: 22017876
    34. mTOR generates an auto-amplification loop by triggering the betaTrCP- and CK1alpha-dependent degradation of DEPTOR. PMID: 22017877
    35. DEPTOR is a novel prognostic marker for differentiated thyroid carcinoma. PMID: 21643629
    36. High expression levels of DEPTOR are predictive of response to thalidomide in myeloma. PMID: 20858096
    37. DEPTOR is highly overexpressed in a subset of multiple myelomas harboring cyclin D1/D3 or c-MAF/MAFB translocations. PMID: 19446321

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  • 数据库链接：

    HGNC: 22953

    OMIM: 612974

    KEGG: hsa:64798

    STRING: 9606.ENSP00000286234

    UniGene: Hs.112981