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  4. Recombinant Human Cyclin-dependent kinase 8 (CDK8)

Recombinant Human Cyclin-dependent kinase 8 (CDK8)

  • 中文名称：
    Recombinant Human Cyclin-dependent kinase 8(CDK8),Yeast
  • 货号：
    CSB-YP005077HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human Cyclin-dependent kinase 8(CDK8),Yeast
  • 货号：
    CSB-EP005077HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human Cyclin-dependent kinase 8(CDK8),Yeast
  • 货号：
    CSB-EP005077HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human Cyclin-dependent kinase 8(CDK8),Yeast
  • 货号：
    CSB-BP005077HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human Cyclin-dependent kinase 8(CDK8),Yeast
  • 货号：
    CSB-MP005077HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CDK8
  • Uniprot No.：
  • 别名：
    cdk8; CDK8 protein kinase; CDK8_HUMAN; Cell Division Kinase 8; Cell division protein kinase 8; Cyclin Dependent kinase 8; Cyclin-dependent kinase 8; K35; Mediator complex subunit cdk8; Mediator of RNA polymerase II transcription subunit cdk8; Protein kinase K35
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-464
  • 氨基酸序列
    MDYDFKVKLS SERERVEDLF EYEGCKVGRG TYGHVYKAKR KDGKDDKDYA LKQIEGTGIS MSACREIALL RELKHPNVIS LQKVFLSHAD RKVWLLFDYA EHDLWHIIKF HRASKANKKP VQLPRGMVKS LLYQILDGIH YLHANWVLHR DLKPANILVM GEGPERGRVK IADMGFARLF NSPLKPLADL DPVVVTFWYR APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED IKTSNPYHHD QLDRIFNVMG FPADKDWEDI KKMPEHSTLM KDFRRNTYTN CSLIKYMEKH KVKPDSKAFH LLQKLLTMDP IKRITSEQAM QDPYFLEDPL PTSDVFAGCQ IPYPKREFLT EEEPDDKGDK KNQQQQQGNN HTNGTGHPGN QDSSHTQGPP LKKVRVVPPT TTSGGLIMTS DYQRSNPHAA YPNPGPSTSQ PQSSMGYSAT SQQPPQYSHQ THRY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
  • 基因功能参考文献：
    1. these observations suggest that Zyxin promotes colon cancer tumorigenesis in a mitotic-phosphorylation-dependent manner and through CDK8-mediated YAP activation. PMID: 29967145
    2. Genes coregulated by CDK8/19 and NFkappaB include IL8, CXCL1, and CXCL2. PMID: 28855340
    3. Moreover, it identified CDK19 and CDK8 to be specifically overexpressed during prostate cancer progression, highlighting their potential as novel therapeutic targets in advanced prostate cancer. PMID: 27678455
    4. identification of the Mediator-associated kinase CDK8, and its paralog CDK19, as negative regulators of IL-10 production during innate immune activation PMID: 28805801
    5. These results identify CDK8 as a novel downstream mediator of estrogen receptors (ER) and suggest the utility of CDK8 inhibitors for ER-positive breast cancer therapy PMID: 28147342
    6. Our data suggest for the first time that CDK8 appears to contribute to the malignant mechanism of LSCC and may represent a significant prognostic marker for LSCC patients. PMID: 28220308
    7. On stimulation of TLR9, CDK8/19 positively regulates inflammatory gene transcription in cooperation with NF-kappaB and C/EBPbeta. PMID: 28151579
    8. Data suggest that, during neurogenesis, Mediator complex cyclin-dependent kinases (CDK8, CDK19) interact directly with PRC2 (polycomb repressive complex 2) subunit EZH2 (enhancer of zeste homolog 2), as well as SUZ12 (suppressor of zeste 12 homolog). PMID: 26002960
    9. the expression of CDK8 and its interactive genes has a profound impact on the response to adjuvant therapy in breast cancer in accordance with the role of CDK8 in chemotherapy-induced tumor-supporting paracrine activities--{REVIEW} PMID: 26452386
    10. Data suggest that MED13, MED12, CDK8 and cyclin C (CycC) comprise a four-subunit "kinase" module of the Mediator complex that functions as a major ingress of oncogenic and developmental signaling/gene expression in humans. [REVIEW] PMID: 26182352
    11. our data provided evidence that CDK-8 played a significant role in colon cancer hepatic metastasis by regulating the Wnt/beta-catenin signal pathway PMID: 26349978
    12. Collectively, our results implicate Ste12 and Tec1 as general and important contributors to the Cdk8, RNAPII-CTD regulatory circuitry as it relates to the maintenance of genome integrity PMID: 26496706
    13. miR-101 is a potent tumor repressor that directly represses CDK8 expression PMID: 26286725
    14. Mediator-associated kinases CDK8 and CDK19 restrain increased activation of key super-enhancer-associated genes in acute myeloid leukaemia (AML) cells PMID: 26416749
    15. The Skp2-mH2A1-CDK8 axis has a critical role in breast cancer development via dysregulation of the G2/M transition, polyploidy, cell growth dysregulation, and loss of tumor suppression. PMID: 25818643
    16. Current state of the art confirms that further development of CDK8 inhibitors will translate into targeted therapies in oncology PMID: 26006748
    17. Microarrays identified target genes for each CDK, and we selected six genes: two target genes of CDK8, two target genes of CDK19 and two genes that were targets for both. PMID: 22117896
    18. Suggest that miR-107 plays a key role in cisplatin resistance by targeting the CDK8 protein in non small cell lung cancer cells. PMID: 25400821
    19. A novel transcriptional repression mechanism of hMED18 mediated by hCDK8 and further a novel positive role of free CDK/cyclin module in transcriptional activation is described. PMID: 24840924
    20. CDK8 regulates the transcription factors turnover, C-terminal domain phosphorylation, and regulates the activator or repressor functions. (Review) PMID: 24139904
    21. Cdk8 is required for stress-induced Mdv1-Dnm1 interaction and mitochondrial fragmentation. PMID: 24439911
    22. Here, loss of CDK8 suppressed the reduced mRNA expression and RNAPII occupancy levels of CTD truncation mutants. PMID: 24009531
    23. CDK8 and CDK19, individually interact with PRMT5 and WDR77, and their interactions with PRMT5 cause transcriptional repression of C/EBPbeta target genes. PMID: 23749998
    24. The phosphorylation of S375 by CDK8 regulates E2F1 ability to repress transcription of beta-catenin/TCF-dependent genes, as well as activation of E2F1-dependent genes. PMID: 22945643
    25. results show a reverse correlation between CDK8 levels and several key features of the endometrial cancer cells, including cell proliferation, migration and invasion as well as tumor formation in vivo PMID: 23454913
    26. Study reports that HIF1A employs a specific variant of the Mediator complex to stimulate RNAPII elongation; the Mediator-associated kinase CDK8, but not the paralog CDK19, is required for induction of many HIF1A target genes. PMID: 23746844
    27. s found that interaction of the CDK8 kinase module with Mediator's middle module interferes with C-terminal domain-dependent RNAPII binding to a previously unknown middle-module CTD-binding site and with the holoenzyme formation process. PMID: 23563140
    28. CDK8 is a key regulator of STAT1 and antiviral responses. PMID: 23352233
    29. 2.2-A crystal structure of CDK8/CycC in complex with sorafenib; the CDK8 activation loop appears not to be phosphorylated. Based on the structure, we discuss an alternate mode of CDK8 activation to the general CDK activation by T-loop phosphorylation PMID: 21806996
    30. Cyclin-dependent kinase 8 mediates chemotherapy-induced tumor-promoting paracrine activities PMID: 22869755
    31. Retroviral cyclin enhances cyclin-dependent kinase-8 activity. PMID: 22379099
    32. we identify a role for the CDK8 oncogene in regulating tumor differentiation and stem cell pluripotency PMID: 22345154
    33. beta-catenin expression was suppressed by CDK8 interference in the gastric adenocarcinoma cell lines. PMID: 21344156
    34. significant inverse correlation between mH2A and CDK8 expression levels exists in melanoma patient samples PMID: 21179167
    35. CDK8 may identify a subset of colon cancer patients with poor prognosis. PMID: 20514474
    36. plays a role in mechanisms of transcriptional regulation upon protein phosphorylation. (review) PMID: 20408451
    37. Overexpression of CDK8 is associated with beta-catenin activation and colon cancer. PMID: 19790197
    38. The s show, using a human tumor cell line, that CDK8 is a positive regulator of genes within the serum response network, including several members of the activator protein 1 and early growth response family of oncogenic transcription factors. PMID: 20098423
    39. CDK8 play positive roles in transcriptional activation. PMID: 17212659
    40. RNA interference experiments demonstrate that CDK8 functions as a coactivator within the p53 transcriptional program. PMID: 17612495
    41. Within T/G-Mediator, cdk8 phosphorylates serine-10 on histone H3, which in turn stimulates H3K14 acetylation by GCN5L within the complex. PMID: 18418385
    42. by retaining RB1 and amplifying CDK8, colorectal tumour cells select conditions that collectively suppress E2F1 and enhance the activity of beta-catenin PMID: 18794899
    43. CDK8 kinase activity was necessary for beta-catenin-driven transformation and for expression of several beta-catenin transcriptional targets PMID: 18794900
    44. Med12--but not Med13--is essential for activating the CDK8 kinase. PMID: 19047373
    45. Med12 and Med13 are critical for human CDK8 subcomplex -dependent repression, whereas CDK8 kinase activity is not PMID: 19240132

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
  • 数据库链接：

    HGNC: 1779

    OMIM: 603184

    KEGG: hsa:1024

    STRING: 9606.ENSP00000370938

    UniGene: Hs.382306