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  4. Recombinant Human Calcitonin gene-related peptide 1 (CALCA),Partial

Recombinant Human Calcitonin gene-related peptide 1 (CALCA),Partial

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  • 中文名称：
    Recombinant Human Calcitonin gene-related peptide 1(CALCA),Partial
  • 货号：
    CSB-RP153444h
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    CALCA
  • Uniprot No.：
  • 别名：
    CALCA; CALC1Calcitonin gene-related peptide 1; Alpha-type CGRP; Calcitonin gene-related peptide I; CGRP-I
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    30.6kDa
  • 表达区域：
    83-118aa
  • 氨基酸序列
    ACDTATCVTHRLAGLLSRSGGVVKNNFVPTNVGSKA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The recombinant Human CALCA protein is made through genetic engineering, also called gene splicing or recombinant DNA technology. By putting Human CALCA genes into the genetic material of the E.coli system. These microorganisms can be used as factories or producers to make proteins for medical, academic and research uses. DNA to be manipulated it must be placed within a “transport vehicle” in which proteins may be produced from the genetic code of the DNA. The host cells used for Human CALCA protein synthesis are E.coli cells, the whole production processes include isolation of CALCA gene, amplification of CALCA gene, cloning, CALCA gene selection, and expression, and the CALCA protein purification, the vector contains N-terminal GST tag in addition to the specific DNA sequence, this facilitates the purification of the recombinant protein and it’s finally detected with a purity of 90%+ by SDS-PAGE.

    CALCA, also called α-CGRP, is a modulatory neuropeptide widely distributed in the central and peripheral nervous system, most in cell bodies of the dorsal root ganglion (DRG). It has inotropic and chronotropic effects. Human α-CGRP is involved in the regulation of vascular tone, wound healing, inflammatory responses, nociception, appetite, and gastric emptying. α-CGRP plays a protective role in a variety of cardiovascular diseases such as heart failure, myocardial infarction, and myocardial ischemia/reperfusion injury (I/R injury).

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.
  • 基因功能参考文献：
    1. CGRP expression in the infrapatellar fat pad of knee osteoarthritis patients is regulated by the COX-2/mPGES-1/PGE2 pathway. PMID: 30205824
    2. PCT may not be sufficiently used as a sole marker of sepsis in neonates compared to CRP. PCT in conjunction with CRP and other tests for septic screen can aid in better diagnosis of neonatal sepsis. PMID: 29567889
    3. Levels of CRP and PCT in CSF did not present greater accuracy in differentiating bacterial from viral meningitis compared to serum levels. PMID: 30143494
    4. Medullary thyroid carcinoma (MTC) was histologically confirmed in all patients with positive procalcitonin (PCT) but not found in patients with negative PCT. Serum PCT levels were significantly higher in patients with MTC than in those without. PMID: 29635700
    5. This is the largest data set evaluating PCT in neonatal and pediatric patients on extracorporeal membrane oxygenation (ECMO). A PCT value of 0.5 ng/mL had the most utility for determining the absence or presence of a bacterial infection in the setting of ECMO with a high sensitivity and negative predictive values. PMID: 29153041
    6. The data shows a subgroup of patients 17/34 (50%) have either elevated PCT or CRP levels. This study is the first to report PCT values in antipsychotic drug-free patients with schizophrenia. PMID: 29723719
    7. Procalcitonin is reliable in discriminating medullary thyroid carcinoma patients with active disease from those with no evidence of disease. PMID: 29707993
    8. This retrospective cohort study aimed to identify the early postoperative kinetics of C-reactive protein (CRP) and procalcitonin (PCT) in children undergoing tetralogy of Fallot (ToF) correction. PMID: 29111402
    9. PCT could be a promising marker of clinical deterioration to septic shock in acute cholangitis. Therefore, PCT might be used as a decision-supporting biomarker for urgent biliary decompression. PMID: 29480416
    10. this study shows that pre-eclampsia affects procalcitonin production in placental tissue PMID: 29427369
    11. peripheral concentration of procalcitonin (both immediate and average levels) might be an independent predictor for prognosis in myocardial infarction patients. PMID: 29179592
    12. Patients with procalcitonin (PCT) above 0.25 mug/L are unlikely to have a unique diagnosis of flu. PMID: 28010128
    13. The cut-off point of procalcitonin level for diagnosing sepsis in the meta-static solid tumor was higher compared to the standard value. PMID: 29382396
    14. PCT is a useful marker for diagnosis of among catheter-related blood stream infection hemodialysis patients. PMID: 28777420
    15. PCT is a useful diagnostic predictor of intra-abdominal infection after colorectal surgery. PMID: 29305753
    16. It is unclear the receptor responsible by CGRP effects, as well as whether this neuropeptide acts directly or indirectly in liver, muscle and adipose tissue--{REVIEW} PMID: 28884411
    17. The aim of the study was to define basal serum calcitonin (bCT) cut-off thresholds for diagnosing medullary thyroid cancer. PMID: 29169190
    18. Serum levels of PCT and PSP are promising biomarkers for early diagnosis of pediatric acute osteomyelitis. PMID: 29091592
    19. Procalcitonin and CRP serum levels are associated with the appearance of intra-abdominal infection after colorectal cancer surgery. PMID: 28918433
    20. PCT distinguished sepsis and non-septic ICU patients. PMID: 28898742
    21. Levels of procalcitonin are significantly elevated in subjects with diabetic foot ulcers and are correlated with clinical indices such as glycemic index and HbA1c level. PMID: 28736121
    22. Serum procalcitonin can be used as a promising single biomarker, easily done in all setup with better accuracy. And it is comparable to computed tomography severity index and Ranson's scores in earlier prediction of severity of acute pancreatitis. PMID: 29446357
    23. PCT showed statistically significant differences between the groups of septic and non-septic burn patients. PMID: 28454850
    24. This study reports the transition dipole strengths and frequencies of the amyloid beta-sheet amide I mode for the aggregated proteins amyloid-beta1-40, calcitonin, alpha-synuclein, and glucagon. PMID: 28851219
    25. Inhibition of SP through use of a neurokinin 1 (NK1) antagonist is highly beneficial following both traumatic brain injury (TBI) and ischaemic stroke in pre-clinical models. The role of CGRP is more unclear, especially with respect to TBI, with both elevations and reductions in CGRP levels reported following trauma PMID: 28817088
    26. targeted non-alanine substitution analysis method and molecular modelling to the functionally important residues of extracellular loop 2 (ECL2) of the calcitonin gene related peptide (CGRP) receptor to reveal key molecular interactions. PMID: 28572046
    27. Patients with lower gastrointestinal perforation (GIP) exhibited significantly higher APACHE II score, SOFA score and serum PCT level than patients with upper GIP. PMID: 28706425
    28. PCT was an accurate predictor for the diagnosis of bacterial infection only in patients with acute liver failure unrelated to acetaminophen intoxication. PMID: 28272093
    29. The maintenance of a high level of procalcitonin within 7 days after surgery and/or a second increase in procalcitonin between the fourth and the seventh postoperative day correlated with postoperative infection after pediatric cardiac surgery. PMID: 28266954
    30. Systemic inflammation moderately occurs in the white coat hypertension. PCT monitoring may be a useful biomarker in inflammation related to atherosclerosis and early stage HT. PMID: 26040439
    31. initial serum levels of procalcitonin might be used as an indicator for aggravation in patients with hepatobiliary tract infection at the emergency department, even though there is hemodynamic stability. PMID: 26096246
    32. Serum procalcitonin levels are elevated in bacteremia. PMID: 28182347
    33. Procalcitonin levels are an early indicator of complications in ICU patients with multiple trauma and patients undergoing major surgery. PMID: 28164563
    34. These results establish that calcitonin gene-related peptide (CGRP) regulates HIV-1 degradation in Langerhans cells (LCs). As CGRP contributes to the sexual response and present within mucosal epithelia, HIV-1 proteasomal degradation in LCs might predominate in vivo and should be enhanced clinically. PMID: 28904199
    35. Diagnostic value of sTREM-1, IL-8, PCT, and CRP in febrile neutropenia after autologous stem cell transplantation PMID: 28920169
    36. PCT and CRP are reliable markers of infection and correlate with infection severity in children with liver disease. PMID: 26933799
    37. The intrapatellar fat pad of knee osteoarthritis patients produces relatively high levels of CGRP. PMID: 28431586
    38. CGRP and IL-4 positively regulated APN/CD13 expression and activity in psoriatic fibroblasts. PMID: 28387421
    39. Elevated levels of procalcitonin in the early phase after pediatric cardiac surgery are associated with increased risk for major adverse events and postoperative renal failure and increased postoperative morbidity. PMID: 27124563
    40. The results indicate a central role of neuroendocrine peptide aminoprocalcitonin (NPCT) in Alzheimer disease pathogenesis and suggest NPCT as a potential biomarker and therapeutic target. PMID: 27497681
    41. Preoperative calcitonin levels were correlated with the presence of tumor, whereas alkaline phosphatase (ALP) levels were not. There were no significant associations between tumor volume and ALP or calcitonin levels in the preoperative or postoperative periods. During long-term follow-up, serum ALP was significantly associated with tumor recurrence, but serum calcitonin was not. PMID: 27922893
    42. PCT median values were significantly higher in patients with sepsis than without during the first 10 postoperative days after cardiac surgery. PMID: 28550906
    43. Point-of-care procalcitonin test can be used to substantially reduce antibiotic exposure in patients hospitalized with acute exacerbation of COPD. PMID: 27382274
    44. Data suggest CGRP receptor (CGRPR) ECL2 (extracellular loop 2 domain) enables interaction with N-terminal residues of CGRP; this provides evidence for dual involvement of ECL2 in two-domain binding model of CGRP/CGRPR interaction; CGRPR is obligate heterodimer of CLR/RAMP1. (CGRP = calcitonin gene-related peptide; CLR = calcitonin receptor-like receptor; RAMP1 = receptor [calcitonin] activity modifying protein 1) PMID: 28691801
    45. Elevated Expression of PCT is associated with Sepsis. PMID: 28005932
    46. Serum procalcitonin levels were not increased in patients with ankylosing spondylitis. PMID: 24798190
    47. Both procalcitonin levels and white blood cell counts were significantly higher in patients with postoperative intraabdominal infections (IAIs) than those in patients without IAIs on postoperative day (POD) 1, POD 3, and POD 5, whereas C-reactive protein levels differed significantly on POD 3 and POD 5. PMID: 27884320
    48. High Procalcitonin expression is associated with engraftment syndrome. PMID: 27762068
    49. In mammalian neuromuscular junctions, different isoforms of exogenously applied calcitonin gene-related peptide uniformly potentiate amplitudes of evoked and spontaneous postsynaptic potentials acting presynaptically via an increase in acetylcholine quantal size. PMID: 27288020
    50. Mouse and human heart valves expressed mRNAs for the CRL ligands adrenomedullin (AM), adrenomedullin-2 (AM-2) and calcitonin gene-related peptide (CGRP) and for their receptor components, i.e., CRL and receptor-activity-modifying proteins 1-3. PMID: 27553639

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Calcitonin family
  • 数据库链接：

    HGNC: 1437

    OMIM: 114130

    UniGene: Hs.37058