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  4. Recombinant Human CCR4-NOT transcription complex subunit 3 (CNOT3)

Recombinant Human CCR4-NOT transcription complex subunit 3 (CNOT3)

  • 中文名称：
    Recombinant Human CCR4-NOT transcription complex subunit 3(CNOT3),Yeast
  • 货号：
    CSB-YP005666HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human CCR4-NOT transcription complex subunit 3(CNOT3),Yeast
  • 货号：
    CSB-EP005666HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human CCR4-NOT transcription complex subunit 3(CNOT3),Yeast
  • 货号：
    CSB-EP005666HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human CCR4-NOT transcription complex subunit 3(CNOT3),Yeast
  • 货号：
    CSB-BP005666HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human CCR4-NOT transcription complex subunit 3(CNOT3),Yeast
  • 货号：
    CSB-MP005666HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CNOT3
  • Uniprot No.：
  • 别名：
    CCR4 associated factor 3; CCR4-associated factor 3; CCR4-NOT transcription complex subunit 3; CNOT3; CNOT3_HUMAN; KIAA0691; LENG2; Leukocyte receptor cluster member 2; negative regulator of transcription 3, S. cerevisiae, homolog of; NOT3 (negative regulator of transcription 3, yeast) homolog; NOT3; NOT3H
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-753
  • 氨基酸序列
    MADKRKLQGE IDRCLKKVSE GVEQFEDIWQ KLHNAANANQ KEKYEADLKK EIKKLQRLRD QIKTWVASNE IKDKRQLIDN RKLIETQMER FKVVERETKT KAYSKEGLGL AQKVDPAQKE KEEVGQWLTN TIDTLNMQVD QFESEVESLS VQTRKKKGDK DKQDRIEGLK RHIEKHRYHV RMLETILRML DNDSILVDAI RKIKDDVEYY VDSSQDPDFE ENEFLYDDLD LEDIPQALVA TSPPSHSHME DEIFNQSSST PTSTTSSSPI PPSPANCTTE NSEDDKKRGR STDSEVSQSP AKNGSKPVHS NQHPQSPAVP PTYPSGPPPA ASALSTTPGN NGVPAPAAPP SALGPKASPA PSHNSGTPAP YAQAVAPPAP SGPSTTQPRP PSVQPSGGGG GGSGGGGSSS SSNSSAGGGA GKQNGATSYS SVVADSPAEV ALSSSGGNNA SSQALGPPSG PHNPPPSTSK EPSAAAPTGA GGVAPGSGNN SGGPSLLVPL PVNPPSSPTP SFSDAKAAGA LLNGPPQFST APEIKAPEPL SSLKSMAERA AISSGIEDPV PTLHLTERDI ILSSTSAPPA SAQPPLQLSE VNIPLSLGVC PLGPVPLTKE QLYQQAMEEA AWHHMPHPSD SERIRQYLPR NPCPTPPYHH QMPPPHSDTV EFYQRLSTET LFFIFYYLEG TKAQYLAAKA LKKQSWRFHT KYMMWFQRHE EPKTITDEFE QGTYIYFDYE KWGQRKKEGF TFEYRYLEDR DLQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity.
  • 基因功能参考文献：
    1. Cnot3 enhances cardiomyocyte proliferation by promoting cell cycle inhibitor mRNA degradation. PMID: 28473716
    2. our findings implicate CNOT3 in the coordination of colonic epithelial cell self-renewal, suggesting this factor as a new biomarker for molecular and prognostic classification of early-stage colorectal cancer PMID: 27899379
    3. A mutation in PRPF31 is hypostatic to a trait acting on CNOT3, with the RP11 phenotype only being observed when there is homozygous (recessive) inheritance of the higher expressivity CNOT3 ("symptomatic" or risk) allele. PMID: 24116917
    4. In asymptomatic carriers CNOT3 is expressed at low levels, allowing higher amounts of wild-type PRPF31 transcripts to be produced and preventing manifestation of retinal degeneration. PMID: 23144630
    5. CNOT3 is a tumor suppressor mutated in 7 of 89 (7.9%) adult T-cell acute lymphoblastic leukemias, and its knockdown causes tumors in a sensitized Drosophila melanogaster model. PMID: 23263491
    6. A high density SNP genotyping approach within the 19q13 chromosome region identifies an association of a CNOT3 polymorphism with ankylosing spondylitis. PMID: 22294640
    7. CNOT3 depletion stabilizes the MAD1 mRNA, and MAD1 knockdown attenuates the CNOT3 depletion-induced increase of the mitotic index. PMID: 22342980
    8. TBP-interacting protein 120B binds to NOT3 PMID: 12207886

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cytoplasm, P-body.
  • 蛋白家族：
    CNOT2/3/5 family
  • 组织特异性：
    Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
  • 数据库链接：

    HGNC: 7879

    OMIM: 604910

    KEGG: hsa:4849

    STRING: 9606.ENSP00000221232

    UniGene: Hs.343571