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  4. Recombinant Human BRCA2 and CDKN1A-interacting protein (BCCIP)

Recombinant Human BRCA2 and CDKN1A-interacting protein (BCCIP)

  • 中文名称：
    Recombinant Human BRCA2 and CDKN1A-interacting protein(BCCIP),Yeast
  • 货号：
    CSB-YP882175HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human BRCA2 and CDKN1A-interacting protein(BCCIP),Yeast
  • 货号：
    CSB-EP882175HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human BRCA2 and CDKN1A-interacting protein(BCCIP),Yeast
  • 货号：
    CSB-EP882175HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human BRCA2 and CDKN1A-interacting protein(BCCIP),Yeast
  • 货号：
    CSB-BP882175HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human BRCA2 and CDKN1A-interacting protein(BCCIP),Yeast
  • 货号：
    CSB-MP882175HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    BCCIP
  • Uniprot No.：
  • 别名：
    bccip; BCCIP_HUMAN; BCCIPalpha; BCCIPbeta; BRCA2 and CDKN1A interacting protein; BRCA2 and CDKN1A-interacting protein; BRCA2 and Cip1/p21 interacting protein; Cdk inhibitor p21 binding protein; P21 and CDK associated protein 1; P21- and CDK-associated protein 1; Protein TOK-1; Protein TOK1; TOK 1; TOK 1alpha; TOK 1beta; TOK1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-314
  • 氨基酸序列
    MASRSKRRAV ESGVPQPPDP PVQRDEEEEK EVENEDEDDD DSDKEKDEED EVIDEEVNIE FEAYSLSDND YDGIKKLLQQ LFLKAPVNTA ELTDLLIQQN HIGSVIKQTD VSEDSNDDMD EDEVFGFISL LNLTERKGTQ CVEQIQELVL RFCEKNCEKS MVEQLDKFLN DTTKPVGLLL SERFINVPPQ IALPMYQQLQ KELAGAHRTN KPCGKCYFYL LISKTFVEAG KNNSKKKPSN KKKAALMFAN AEEEFFYEKA ILKFNYSVQE ESDTCLGGKW SFDDVPMTPL RTVMLIPGDK MNEIMDKLKE YLSV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    During interphase, required for microtubule organizing and anchoring activities. During mitosis, required for the organization and stabilization of the spindle pole. Isoform 2/alpha is particularly important for the regulation of microtubule anchoring, microtubule stability, spindle architecture and spindle orientation, compared to isoform 1/beta. May promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ).
  • 基因功能参考文献：
    1. We analyzed the BCCIP protein level in 470 cases of human breast cancer to determine the associations between BCCIP and 53BP1, p53, and subtypes of breast cancer. We further constructed a unique BCCIP knockdown mouse model to determine whether a partial BCCIP deficiency leads to spontaneous breast cancer formation PMID: 29047390
    2. BCCIPbeta, as a S7 modulator, contributes to the regulation of ribosomal and extraribosomal functions of S7 and has implications in cell growth and tumor development. PMID: 28510697
    3. this study identifies BCCIP as a novel factor critical for microtubule regulation and explicates a mechanism utilized by BCCIP in tumor suppression. PMID: 28394342
    4. Expression of BCCIP beta was notably higher in tumor tissues of esophageal squamous cell carcinoma (ESCC) and Eca 109 cells. In vitro studies such as starvation and refeeding assay along with BCCIP beta-shRNA transfection assay demonstrated that BCCIP beta expression promoted proliferation of ESCC cells. PMID: 27995408
    5. we demonstrate that BCCIPbeta induces a conformational change within the RAD51 filament that promotes release of ADP to help maintain an active presynaptic filament. Our findings reveal a functional role for BCCIPbeta as a RAD51 accessory factor in HR. PMID: 27694622
    6. The BCCIPbeta, but not BCCIPalpha, interacts with EIF6 and RPL23/UL14 and stabilizes the latter. PMID: 25150171
    7. Results indicate that BCCIP germ line mutations are unlikely to be a major contributor to familial breast/ovarian cancer risk in our population. PMID: 23911796
    8. In BRCA2, two novel frame shift mutations were identified as 5073-5074delCT and 6866delC. PMID: 11748848
    9. BRCA2 germline mutations appear to have a milder clinical phenotype when compared to non-BRCA2 mutations, since survival is higher among breast cancer patients carrying a BRCA2 mutation compared to sporadic breast cancers. PMID: 11754111
    10. Unique de novo mutation of BRCA2 has been identified in a woman with early onset breast cancer. PMID: 11836363
    11. Overexpression of BCCIP beta delays the G1 to S progression and results in an elevated p21 expression PMID: 14726710
    12. BCCIP is critical for BRCA2- and RAD51-dependent responses to DNA damage and homologous recombinational repair. PMID: 15713648
    13. BCCIP in maintaining the transactivation activity of wild type p53; down-regulation of BCCIP as a novel mechanism to impair the p53 function in cells harboring wild type p53 PMID: 17135243
    14. suggest an essential role of BCCIP in the maintenance of genomic integrity PMID: 17452982
    15. BCCIP-dependent repair of double strand breaks by homologous recombination is an early RAD51 response to ionizing radiation-induced DNA damage. PMID: 18413737
    16. LYRIC/AEG-1 is a negative regulator of BCCIPalpha, promoting proteasomal degradation either through direct interaction, or potentially through an indirect mechanism involving downstream effects of the NF-kappaB signaling pathway. PMID: 18440304
    17. BCCIP is phosphorylated by the Src tyrosine kinase and dephosphorylated by the PTPmu tyrosine phosphatase; neurite outgrowth assays suggest that BCCIP and PTPmu are in a common signal transduction pathway. PMID: 18773424
    18. BCCIP protein expression was not detectable in approximately 45% of all astrocytic tumors. PMID: 19653894
    19. regulation of p21 intracellular distribution is a new mechanism for BCCIP to modulate p21 functions PMID: 19713748

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  • 亚细胞定位：
    Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, spindle pole.; [Isoform 1]: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.; [Isoform 2]: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
  • 蛋白家族：
    BCP1 family
  • 组织特异性：
    Expressed at high levels in testis and skeletal muscle and at lower levels in brain, heart, kidney, liver, lung, ovary, pancreas, placenta, and spleen.
  • 数据库链接：

    HGNC: 978

    OMIM: 611883

    KEGG: hsa:56647

    STRING: 9606.ENSP00000357748

    UniGene: Hs.370292