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  4. Recombinant Human Alpha-tocopherol transfer protein (TTPA)

Recombinant Human Alpha-tocopherol transfer protein (TTPA)

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  • 中文名称：
    人TTPA重组蛋白
  • 货号：
    CSB-EP025268HU
  • 规格：
    ￥1836
  • 促销：
    促销单品低至599元起
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    TTPA
  • Uniprot No.：
  • 别名：
    Alpha-tocopherol transfer protein; Alpha-TTP; AlphaTTP; ATTP; AVED; Tocopherol (alpha) transfer protein (ataxia (Friedreich-like) with vitamin E deficiency); TTP1; TTPA; TTPA_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    47.7kDa
  • 表达区域：
    1-278aa
  • 氨基酸序列
    MAEARSQPSAGPQLNALPDHSPLLQPGLAALRRRAREAGVPLAPLPLTDSFLLRFLRARDFDLDLAWRLLKNYYKWRAECPEISADLHPRSIIGLLKAGYHGVLRSRDPTGSKVLIYRIAHWDPKVFTAYDVFRVSLITSELIVQEVETQRNGIKAIFDLEGWQFSHAFQITPSVAKKIAAVLTDSFPLKVRGIHLINEPVIFHAVFSMIKPFLTEKIKERIHMHGNNYKQSLLQHFPDILPLEYGGEEFSMEDICQEWTNFIMKSEDYLSSISESIQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds alpha-tocopherol, enhances its transfer between separate membranes, and stimulates its release from liver cells. Binds both phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 4,5-bisphosphate; the resulting conformation change is important for the release of the bound alpha-tocopherol.
  • 基因功能参考文献：
    1. alphaTTP does not appear to regulate the uptake and intracellular localization of different vitamin E congeners in cultured liver cells. PMID: 30098456
    2. These findings provide the basis for a proposed mechanistic model that describes TTP-facilitated trafficking of alpha-tocopherol through hepatocytes. PMID: 27307040
    3. Current results show that alphaTTP plays a role in endometrial carcinoma. Possibly endometrial cancer cells attempt to protect themselves from increasing oxidative stress by up-regulating alphaTTP. PMID: 28213729
    4. Single-nucleotide polymorphisms that are commonly found in healthy humans dramatically affect promoter activity of the TTPA gene. PMID: 23079030
    5. The crystal structure of the alpha-TTP-phosphatidylinositol phosphates (PIPs) complex revealed that the familial vitamin E deficiency-related arginine residues interacted with phosphate groups of the PIPs and that the PIPs binding caused the lid of the alpha-tocopherol-binding pocket to open. PMID: 23599266
    6. study demonstrated that alpha -TTP can be upregulated in case of oxidative stress in BeWo trophoblast cells; speculate that possibly, alpha -TTP is notonly involved in normal pregnancy, but also in cases of pregnancy disorders with intense oxidative stress PMID: 22752767
    7. Data show that reduction ("knockdown") of tocopherol transfer protein (TTP) expression resulted in resistance to the vitamin E. PMID: 20826775
    8. Substitution of residues in helices A8 (F165A and F169A) and A10 (I202A, V206A and M209A) decreased the rate of intermembrane ligand transfer as well as protein adsorption to phospholipid bilayers. PMID: 21110980
    9. First case of a mutated form of the TTPA gene in a patient also carrying a spinocerebellar ataxia type 8 expansion. PMID: 12470185
    10. crystal structure reveals two conformations PMID: 12899840
    11. the positively charged surface of TTPA may serve to orient an interacting protein, which might function to regulate the release of alpha-T through an induced change in conformation of ATTP PMID: 14657365
    12. Findings suggest the possibility that ataxia with vitamin E deficiency syndrome (AVED) may not arise from an inability of TTP to bind or to transfer alpha tocopherol, but rather from defects in other activities of the protein. PMID: 15065857
    13. Nuclear localization of TTPA in in trophoblast, fetal capillaries' endothelium and amnion epithelium of human term placentamay represent a novel function of TTPA PMID: 15190938
    14. In Ataxia with vitamin E deficiency two TTPA mutations were identified: a truncating mutation in a homozygous patient, and a Gly246Arg missense mutation in a compound heterozygous patient associated with a mild and slowly progressive form of the disease. PMID: 15300460
    15. The physiological role of TTP is anchored in its ability to direct vitamin E trafficking from the endocytic compartment to transport vesicles that deliver the vitamin to the site of secretion at the plasma membrane. PMID: 16819822
    16. analysis of ligand transfer by the hepatic tocopherol transfer protein PMID: 18458085
    17. in first-trimester extravillous trophoblast, syncytiotrophoblast and amniotic epithelium PMID: 18511174
    18. The vitamin E deficiency with hereditary motor neuropathy was found to be homozygous for a 513insTT mutation in exon 3 of the alpha-tocopherol transfer protein gene. PMID: 18984846
    19. Total deletion of the TTPA gene is expected to be associated with severe phenotype in AVED patients. PMID: 19102053
    20. Genetic variation in TTPA and SEC14L2 is associated with serum alpha-tocopherol but does not have a direct effect on prostate cancer when vitamin E is administered. PMID: 19190344
    21. Hydrophobic features of alpha-TTP dominating the binding energy between protein and membrane. PMID: 19458973

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  • 相关疾病：
    Ataxia with isolated vitamin E deficiency (AVED)
  • 亚细胞定位：
    Cytoplasm.
  • 数据库链接：

    HGNC: 12404

    OMIM: 277460

    KEGG: hsa:7274

    STRING: 9606.ENSP00000260116

    UniGene: Hs.69049