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  4. Recombinant Human Acidic mammalian chitinase (CHIA)

Recombinant Human Acidic mammalian chitinase (CHIA)

  • 中文名称：
    Recombinant Human Acidic mammalian chitinase(CHIA)
  • 货号：
    CSB-EP874858HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    CHIA
  • Uniprot No.：
  • 别名：
    Acidic mammalian chitinase [Precursor]; Acidic mammalian chitinase; AMCase; Chia; CHIA_HUMAN; CHIT2; chitinase; acidic; DKFZp313J1722; ECF L; Lung specific protein TSA1902; Lung-specific protein TSA1902; RP5 1125M8.1; TSA1902
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Isoform 2
  • 来源：
    E.coli
  • 分子量：
    67.1kDa
  • 表达区域：
    1-368aa
  • 氨基酸序列
    MVSTPENRQTFITSVIKFLRQYEFDGLDFDWEYPGSRGSPPQDKHLFTVLVQEMREAFEQEAKQINKPRLMVTAAVAAGISNIQSGYEIPQLSQYLDYIHVMTYDLHGSWEGYTGENSPLYKYPTDTGSNAYLNVDYVMNYWKDNGAPAEKLIVGFPTYGHNFILSNPSNTGIGAPTSGAGPAGPYAKESGIWAYYEICTFLKNGATQGWDAPQEVPYAYQGNVWVGYDNIKSFDIKAQWLKHNKFGGAMVWAIDLDDFTGTFCNQGKFPLISTLKKALGLQSASCTAPAQPIEPITAAPSGSGNGSGSSSSGGSSGGSGFCAVRANGLYPVANNRNAFWHCVNGVTYQQNCQAGLVFDTSCDCCNWA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding.
  • 基因功能参考文献：
    1. rs10494132 polymorphism of CHIA might be a risk factor for asthma. PMID: 29233108
    2. we showed that M at the position 61 is highly conserved in the many mammals other than orangutan and humans. In addition, introduction of M61R and M61I to WT mouse AMCase lead to a significant reduction of its chitinolytic activity. Taken together, our present and previous data by Seibold et al. (2009) indicate that G339T (R61M) in humans is associated with asthma protection in the fashion of gain of function PMID: 27702777
    3. This is the first report on the association between low-frequency and common variants in the chitinases-related genes CHIA and CHI3L1 with the intensity of specific IgE to ABA-1 in a population naturally exposed to Ascaris and with Bet v 1 in a Swedish population. PMID: 27977724
    4. These data provide the experimental evidence that telomere shortening and related inflammatory proteins are associated with human IgAN, and it could be a new direction for the disease progression study. PMID: 24903994
    5. loss of acidic chitinase expression is a promising marker for corpus atrophy PMID: 24119614
    6. results showed that the expression of AMCase and CHIT-1 were differently modulated in monocyte macrophages at different stage of maturation. The behavior of these two active chitinase suggests that in the immune response their role is complementary. PMID: 23129258
    7. study demonstrated genetic associations between chitinase gene variants and lung function level and rate of decline in chronic obstructive pulmonary disease patients from the Lung Health Study; also functional effect of the rs3818822 polymorphism on AMCase levels and activity was demonstrated PMID: 22200767
    8. increased chitinolytic activity in nasal polyps PMID: 21711963
    9. Report increased CSF chitinase levels in patients with neuromyelitis optica and relapsing remitting multiple sclerosis. PMID: 21159721
    10. A ten base pair insertion in the second exon in the 5'UTR region of the AMCase gene may modify the gene expression and thus may affect the severity of asthma. PMID: 21511453
    11. Results showed the presence of CHIT1 and AMCase mRNA in gastric mucosa and the correlation with the presence of H. pylori was significant only for CHIT1 but not for AMCase expression. PMID: 19242357
    12. Environmental exposure to fungi does not modify the effect of CHIA SNPs on severe asthma exacerbations PMID: 20538957
    13. The levels of expression of AMCase and chitotriosidase mRNAs and proteins were increased in allergic turbinate mucosa compared with normal turbinate mucosa. PMID: 20422678
    14. Our study found no associations between single nucleotide polymorphisms in the genes CHIT1, CHIA, and CHI3L1 and asthma, changes in lung physiology, or allergy-related phenotypes in subjects with mild-to-moderate asthma PMID: 20226308
    15. expressed in exaggerated quantities in human asthma PMID: 15192232
    16. human AMCase has only one pH optimum for k(cat)/K(m) between pH 4 and 5. Steady state kinetics shows that human AMCase has "low" intrinsic transglycosidase activity, which leads to the observation of apparent substrate inhibition. PMID: 16584180
    17. results establish a significant association of CHIA with atopic asthma and serum total IgE levels in the Indian population PMID: 18602573
    18. lung epithelial cells secrete AMCase via an EGFR-dependent pathway that is activated by ADAM17 and mediates its effects via Ras. PMID: 18824549
    19. The three-dimensional (3D) model of the human acidic mammalian chitinase (hAMCase) was constructed based on the crystal structure of the human chitotriosidase (EC 3.2.1.44, PDB code 1HKK) by using InsightII/Homology module. PMID: 19085022
    20. secreted AMCase feeds back in an autocrine and/or paracrine manner to protect pulmonary epithelial cells from growth factor withdrawal- and Fas ligand-induced apoptosis PMID: 19342690
    21. Genetic association analyses of AMCase haplotypes for asthma revealed significant protective associations between the variant haplotype in several asthma cohorts. PMID: 19435888
    22. novel asthma susceptibility genes; review of genetic and fuctional studies PMID: 19644363

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  • 亚细胞定位：
    [Isoform 1]: Secreted. Note=Secretion depends on EGFR activity.; [Isoform 2]: Cytoplasm.; [Isoform 3]: Cytoplasm.
  • 蛋白家族：
    Glycosyl hydrolase 18 family, Chitinase class II subfamily
  • 组织特异性：
    Detected in lung epithelial cells from asthma patients (at protein level). Highly expressed in stomach. Detected at lower levels in lung.
  • 数据库链接：

    HGNC: 17432

    OMIM: 606080

    KEGG: hsa:27159

    STRING: 9606.ENSP00000341828

    UniGene: Hs.128814