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  4. Recombinant Human 26S proteasome non-ATPase regulatory subunit 4 (PSMD4)

Recombinant Human 26S proteasome non-ATPase regulatory subunit 4 (PSMD4)

  • 中文名称：
    人PSMD4重组蛋白
  • 货号：
    CSB-YP018908HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PSMD4重组蛋白
  • 货号：
    CSB-EP018908HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PSMD4重组蛋白
  • 货号：
    CSB-EP018908HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PSMD4重组蛋白
  • 货号：
    CSB-BP018908HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PSMD4重组蛋白
  • 货号：
    CSB-MP018908HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PSMD4
  • Uniprot No.：
  • 别名：
    26S protease subunit S5a; 26S proteasome non ATPase regulatory subunit 4; 26S proteasome non-ATPase regulatory subunit 4; 26S proteasome regulatory subunit rpn10; 26S proteasome regulatory subunit S5A; AF 1; AF; AF1; Angiocidin; Antisecretory factor 1; ASF; DS5a; MCB 1; MCB1; Multiubiquitin chain binding protein; Multiubiquitin chain-binding protein; OTTHUMP00000059963; Prosome macropain; Proteasome (prosome macropain) 26S subunit non ATPase 4; Proteasome 19S S5A; Proteasome 26S non ATPase subunit 4; Proteasome 26S subunit non ATPase 4; PSMD 4; Psmd4; PSMD4_HUMAN; pUB R5; pUBR5; Rpn 10; Rpn10; RPN10 homolog; S5A; S5a/antisecretory factor protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-377
  • 氨基酸序列
    MVLESTMVCV DNSEYMRNGD FLPTRLQAQQ DAVNIVCHSK TRSNPENNVG LITLANDCEV LTTLTPDTGR ILSKLHTVQP KGKITFCTGI RVAHLALKHR QGKNHKMRII AFVGSPVEDN EKDLVKLAKR LKKEKVNVDI INFGEEEVNT EKLTAFVNTL NGKDGTGSHL VTVPPGPSLA DALISSPILA GEGGAMLGLG ASDFEFGVDP SADPELALAL RVSMEEQRQR QEEEARRAAA ASAAEAGIAT TGTEDSDDAL LKMTISQQEF GRTGLPDLSS MTEEEQIAYA MQMSLQGAEF GQAESADIDA SSAMDTSEPA KEEDDYDVMQ DPEFLQSVLE NLPGVDPNNE AIRNAMGSLA SQATKDGKKD KKEEDKK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.
  • 基因功能参考文献：
    1. Preterm delivery is associated with low levels of anti-secretory factor in placenta. Inflammation, a potential trigger of preterm birth, is more pronounced in the preterm placenta and inversely related to the placental level of anti-secretory factor. PMID: 29265188
    2. We therefore suggest that PSMD4 or b-catenin might be potential targets for suppressing tumor aggressiveness, and consequently, improving outcomes in patients whose tumors express cNrf2. PMID: 27033953
    3. Binding (especially high-affinity binding) of non-ubiquitinated proteins to the Rpn10 proteasome subunit can both regulate the functioning of this proteasomal ubiquitin receptor (by competing with ubiquitinated substrates) and promote activation of other pathways for proteolytic degradation of proteins destined to the proteasome. PMID: 28988533
    4. The platelet 26S proteasome exhibits different basal activities of its catalytic subunits and chymotrypsin-like activity is most prominently enhanced by calcium dependent signaling. Collagen stimulation enhances 26S proteasome chymotrypsin-like activity in platelets. PMID: 27768934
    5. regulation of NY-ESO-1 processing by the ubiquitin receptors Rpn10 and Rpn13 as a well as by the standard and immunoproteasome is governed by non-canonical ubiquitination on non-lysine sites. PMID: 26903513
    6. Not only AF1, but an entire proteasome complex, seems to be present in blood. PMID: 25897558
    7. binds to death receptor-6, and induces monocyte cell line differentiation via NF-kB pathway PMID: 24829148
    8. The VWA domain regulation of ubiquitin and Ubl binding to S5a is restricted to the 26S proteasome. PMID: 25318673
    9. The degradation of TP53 and MDM2 by the proteasome can be selectively dependent on S5a in human cells. PMID: 24121268
    10. To examine angiocidin expression in SMMC-7221 and HepG2 cells and the role of angiocidin in liver cancer cell growth. Angiocidin is highly expressed in liver cancer cells, and it may play a key role in tumor growth of liver cancers. PMID: 24250289
    11. s demonstrate that human cytomegalovirus UL76 induces a novel nuclear aggresome, likely by subverting S5a of the ubiquitin-proteasome system. PMID: 23966401
    12. One consequence of the interaction between E6/E6AP and S5a is enhanced ubiquitination of this proteasome subunit. PMID: 24074603
    13. These studies suggest that diminished 26S activity in failing human hearts may be related ti impaired docking of the 19S to the 20S as a result of decreased Rpt subunit ATPase activity and alpha7 subunit phosphorylation. PMID: 23515276
    14. identified the VWA domain of hRpn10 as a receptor for ubiquitin-like proteins within the 26S proteasome and elucidated how FAT10 mediates efficient proteolysis by the proteasome PMID: 22434192
    15. Both higher PSMD4 expression levels and higher 1q21 copy numbers affected clinical outcome adversely. PMID: 21628408
    16. results suggest that there is different substrate specificity between S5a and hRpn13 at the level of delivery and S5a may be the major docking site for ERAD substrates. PMID: 20417181
    17. Overexpression of the S5a subunit of proteasome activator PA700 did not recover proteasome function in Huntington disease cells. S5a. PMID: 17327906
    18. parkin Ubld uses differential surfaces to recruit UIM regions from the S5a proteasomal subunit compared with Eps15 involved in cell signaling. PMID: 19875440
    19. S5a component of the 19S complex interacts with different ubiquitin-like (ubl) modules PMID: 11827521
    20. HHr23a binds to this protein, which changes its own conformation. PMID: 14557549
    21. structure of the ubiquitin-interacting motif of the proteasome subunit S5a bound to the ubiquitin-like domain of HR23B PMID: 14585839
    22. Results report the structure of S5a (196-306) alone and complexed with two monoubiquitin molecules, and present a model for how S5a and other ubiquitin-binding proteins recognize polyubiquitin. PMID: 15826667
    23. These results are consistent with the conclusion that the anti-tumor activity of angiocidin arises from its ability to ligate collagen and alpha2beta1 on endothelial cells and tumor cells. PMID: 16762342
    24. UIM2 domain of S5a binds preferentially to hHR23a over polyubiquitin, and a model is provided for the ternary complex that represents one of the mechanisms used by the proteasome to capture ubiquitylated substrates. PMID: 17408689
    25. results suggest that S5a is regulated during apoptosis at the transcriptional level and S5a upregulation by antiapoptotic signals can contribute to cell survival. PMID: 17459097
    26. S5a-UIMs can be used as upstream inhibitors of the proteasome pathway. PMID: 17949686
    27. In autoimmune disorders like MS, angiocidin activates T cells, macrophages, microglia & astrocytes to produce inflammatory cytokines and to stimulate antigen presentation. PMID: 18207252
    28. angiocidin activates monocytes to secrete cytokines and differentiates them to a macrophage-like phenotype through at least two pathways mediated by MAPK and NF-kappaB, as well as PI3K PMID: 18632645
    29. The presence of S5a in the reaction containing a substrate (luciferase), ubiquitination enzymes (an E2, UbcH5, and the U-box E3 ligase CHIP) and 26S proteasome significantly increased the rate of luciferase degradation. PMID: 19387488
    30. Angiocidin's ability to elicit tumor cell death may be mediated in part by it's pro-inflammatory effects on immune cells in the tumor microenvironment. PMID: 19519434
    31. The binding of S5a to K48-linked diubiquitin is defined at an atomic level resolution. PMID: 19683493
    32. Proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (also called subunit 5a) has two independent polyubiquitin binding sites whose sequences are highly conserved among higher eukaryotic orthologs. PMID: 9488668

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  • 蛋白家族：
    Proteasome subunit S5A family
  • 数据库链接：

    HGNC: 9561

    OMIM: 601648

    KEGG: hsa:5710

    STRING: 9606.ENSP00000357879

    UniGene: Hs.505059