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  4. Recombinant Human 2-5A-dependent ribonuclease (RNASEL), partial

Recombinant Human 2-5A-dependent ribonuclease (RNASEL), partial

  • 中文名称：
    人RNASEL重组蛋白
  • 货号：
    CSB-EP313223HU1
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    2 5A dependent ribonuclease; 2 5A dependent RNase; 2' 5' oligoisoadenylate synthetase dependent; 2-5A-dependent ribonuclease; 2-5A-dependent RNase; DKFZp781D08126; Interferon induced 2 5A dependent RNase; MGC104972; MGC133329; PRCA 1; PRCA1; Ribonuclease 4; Ribonuclease L (2' 5' oligoisoadenylate synthetase dependent) ; Ribonuclease L; Ribonuclease4; RibonucleaseL; RN5A_HUMAN; RNase L; Rnasel; RNS 4; RNS4
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    63.4 kDa
  • 表达区域：
    330-741aa
  • 氨基酸序列
    HPPAEDWKPQSSHWGAALKDLHRIYRPMIGKLKFFIDEKYKIADTSEGGIYLGFYEKQEVAVKTFCEGSPRAQREVSCLQSSRENSHLVTFYGSESHRGHLFVCVTLCEQTLEACLDVHRGEDVENEEDEFARNVLSSIFKAVQELHLSCGYTHQDLQPQNILIDSKKAAHLADFDKSIKWAGDPQEVKRDLEDLGRLVLYVVKKGSISFEDLKAQSNEEVVQLSPDEETKDLIHRLFHPGEHVRDCLSDLLGHPFFWTWESRYRTLRNVGNESDIKTRKSESEILRLLQPGPSEHSKSFDKWTTKINECVMKKMNKFYEKRGNFYQNTVGDLLKFIRNLGEHIDEEKHKKMKLKIGDPSLYFQKTFPDLVIYVYTKLQNTEYRKHFPQTHSPNKPQCDGAGGASGLASPGC
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis. Might play a central role in the regulation of mRNA turnover. Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length.
  • 基因功能参考文献：
    1. Mitochondrial C11orf83 is a potent antiviral protein independent of interferon production; and knockdown of either OAS3 or RNase L impaired the antiviral capability of C11orf83. PMID: 28418037
    2. Our study suggests that RNASEL:p.Glu265* may be a genetic modifier of risk for early-onset breast cancer predisposition in carriers of high-risk mutations. PMID: 29422015
    3. Results reported in the present study suggest a sex-specific interaction between miR-146a and RNASEL genes in melanoma skin cancer susceptibility. PMID: 28654546
    4. These results demonstrate that ablation of RNase L activity promotes survival of ADAR1 deficient cells even in the presence of MDA5 and MAVS, suggesting that the RNase L system is the primary sensor pathway for endogenous dsRNA that leads to cell death. PMID: 28362255
    5. Mutations in the genes glucokinase regulatory protein (GCKR), RNase L (RNASEL), leukocyte immunoglobulin-like receptor 3 (LILRA3), and dynein axonemal heavy chain 10 (DNAH10) segregated with elevated HDLc levels in families, while no mutations associated with low HDLc. PMID: 24891332
    6. findings suggest that beside the RLR pathway, RNase L cleavage products can also activate the NLRP3-inflammasome pathway, which requires DHX33 (DExD/H-box helicase) and the mitochondrial adaptor protein MAVS. PMID: 26987611
    7. This study provides the evidence that germline variations in RNASEL are associated with fatal PCa in men (Gleason score >7 for rs486907 and RNASEL underexpressed [P = 0.007] in patients with PCa). PMID: 27318894
    8. By sequencing abundant RNA fragments generated by RNase L in cell lines, we identify site-specific cleavage of two groups of noncoding RNAs: Y-RNAs, whose function is poorly understood, and cytosolic tRNAs, which are essential for translation. PMID: 28808124
    9. RNASEL rs3738579 genotype was significantly related to severe necroinflammatory activity (NIA) grade of chronic hepatitis C patients. PMID: 28704535
    10. Serum RNase-L levels were inversely associated with metabolic syndrome and age. PMID: 28399925
    11. We show that mutations in RNase L found in HPC patients may promote prostate cancer by increasing expression of AR-responsive genes and cell motility and identify novel roles of RNase L as a prostate cancer susceptibility gene. PMID: 28257035
    12. Suggest that naturally occurring mutations in the RNase L gene might promote enhanced prostate cancer cell migration and metastasis. PMID: 26517238
    13. This review outlines the role of RNase-L in antimicrobial immunity and the cytoskeleton-associated innate response. [review] PMID: 26760998
    14. Data show that RNA decay by ribonuclease L (RNase L) has an important role in homeostasis and serves as a suppressor of cell adhesion. PMID: 26668391
    15. Single Nucleotide Polymorphisms in RNASEL involved in the immune response are generally not associated with intraprostatic inflammation in men without a Prostate cancer diagnosis. PMID: 26771888
    16. OAS3 displayed a higher affinity for dsRNA in intact cells than either OAS1 or OAS2, consistent with its dominant role in RNase L activation. PMID: 26858407
    17. Tanslation of vaccinia virus A27L and B5R genes is independent of PKR activation, but their expression is dependent on the RNase L activity. PMID: 26656695
    18. Our results demonstrate a novel role of RNase L generated small RNAs in cross-talk between autophagy and apoptosis that impacts the fate of cells during viral infections and cancer. PMID: 26263979
    19. Among 794 RNASEL Ssingle nucleotide polymorphism (SNPs) entries 124 SNPs were found nonsynonymous (ns) from which SIFT predicted 13 nsSNPs as nontolerable whereas PolyPhen-2 predicted 28. PMID: 26236721
    20. Virus infection and RNase L activation disrupt its association with Filamin A and release RNase L to mediate its canonical nuclease-dependent antiviral activities. PMID: 25352621
    21. These data show that RNase L targets specific sites in both host and viral RNAs to restrict influenza virus replication when NS1 protein is disabled. PMID: 25540362
    22. The IFNs inhibit viral infections in part through the 2',5'-oligoadenylate (2-5A) synthetase (OAS)/RNase L pathway. PMID: 24905202
    23. a model of feedback regulation in which RNase L and TTP target SRF mRNA and SRF-induced transcripts. PMID: 25301952
    24. data suggest that RNASEL, an enzyme involved in RNA turnover, is controlled by miR-146a and may be important in NMSC etiology PMID: 24699816
    25. The combined high affinity for double-stranded RNA and the capability to produce 2'-5'-linked oligoadenylates of sufficient length to activate RNase L suggests that OAS3 is a potent activator of RNase L. PMID: 25275129
    26. This study identified three RNASEL variants that are associated with risk for prostate cancer. PMID: 21360564
    27. RNAse L gene SNP 1385G>A does not have a clear clinical significance in CHC. PMID: 25286525
    28. RNase L activity limits FFA/obesity-induced impairment of insulin response in muscle cells via TLR3 and MnSOD expression. PMID: 24651439
    29. This work identifies novel roles for IFN-beta and RNase L in cell barrier functions that are targeted by bacterial effectors to escape host defense mechanisms and promote virulence. PMID: 24733098
    30. Association between SNPs from RNASEL and chromosome 8q24 with the risk of prostate cancer, and its aggressiveness, in a Hispanic (Chilean) population. PMID: 24224612
    31. this study reports 2.8 and 2.1 angstrom crystal structures of human RNase L in complexes with synthetic and natural ligands and a fragment of an RNA substrate. PMID: 24578532
    32. We show an association between RNASEL SNP rs12757998 and outcome after radiation therapy for prostate cancer PMID: 23382116
    33. the structural changes of human RNase L as a result of interactions with four different activators: natural 2-5 pA(4) and three tetramers with 3'-end AMP PMID: 22691533
    34. Data show that miR-29 acts via 4 target sites within the RNASEL 3' UTR. PMID: 23113544
    35. RNASEL mutations are associated with xenotropic murine leukemia virus and its R426Q polymorphism in patients with prostate cancer PMID: 23098452
    36. Two molecules of 2-5A at a time tether RNase L monomers via the ankyrin-repeat (ANK) domain. Each ANK domain harbors two distinct sites for 2-5A recognition that reside 50 A apart. PMID: 23084743
    37. among the members of the OAS family, OAS1 p46 and OAS3 p100 mediate the RNase L-dependent antiviral activity against HCV PMID: 23196181
    38. RNase L induces autophagy via c-Jun N-terminal kinase and double-stranded RNA-dependent protein kinase signaling pathways. PMID: 23109342
    39. study found that RNase L is highly expressed in lung cancer cells with significantly decreased enzymatic activity due to an increase of RLI, a specific inhibitor of RNase L PMID: 22925698
    40. Positive selection has operated on the RNASEL gene. PMID: 22513284
    41. Statistically significant differences were found between controls and patients in some of the genotyped regions of the RNASEL gene PMID: 22464196
    42. genotypes associated with the worst prognoses in prostate cancer are G/G in D541E, A/A in R462Q and A/G in I97L. PMID: 22083266
    43. The RNASEL 541Gln allele might be a low-penetrent risk factor for sporadic prostate cancer. PMID: 21656378
    44. Five SNPs were validated as being significantly associated with prostate cancer mortality, one each in the LEPR, CRY1, RNASEL, IL4, and ARVCF genes. PMID: 21846818
    45. The RNASEL -1385G/A polymorphism is associated with cancer risk in African descendents. PMID: 21221811
    46. Ribonuclease L (RNASEL) protein was shown to be up-regulated in lopinavir-treated SiHa cells, which was confirmed by PCR and western blot. Targeted silencing of RNASEL reduced the sensitivity of SiHa cells to lopinavir. PMID: 21685539
    47. Codon 462 polymorphisms within the RNASEL gene are not associated with an increased risk of cervical cancer. PMID: 21665181
    48. Data show that the IQ motif-containing Ras GTPase-activating-like protein 1 (IQGAP1) can associate with RNase L, and that phosphorylation occurs on the IQGAP1, and works as a regulator in apoptosis. PMID: 20875083
    49. Genetic variation is associated with RNASEL associated with prostate cancer. PMID: 20576793
    50. These studies suggest that the expression of Xpr1 and certain genotypes of the RNASEL gene, which could restrict XMRV infection, may play important roles in defining XMRV tropisms in certain cell types. PMID: 20410264

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  • 相关疾病：
    Prostate cancer, hereditary, 1 (HPC1)
  • 亚细胞定位：
    Cytoplasm. Mitochondrion.
  • 蛋白家族：
    Protein kinase superfamily
  • 组织特异性：
    Highly expressed in spleen and thymus followed by prostate, testis, uterus, small intestine, colon and peripheral blood leukocytes.
  • 数据库链接：

    HGNC: 10050

    OMIM: 176807

    KEGG: hsa:6041

    STRING: 9606.ENSP00000356530

    UniGene: Hs.518545