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  4. Recombinant Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 (PLCD1)

Recombinant Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 (PLCD1)

  • 中文名称：
    人PLCD1重组蛋白
  • 货号：
    CSB-YP018130HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PLCD1重组蛋白
  • 货号：
    CSB-EP018130HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PLCD1重组蛋白
  • 货号：
    CSB-EP018130HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PLCD1重组蛋白
  • 货号：
    CSB-BP018130HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PLCD1重组蛋白
  • 货号：
    CSB-MP018130HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    PLCD1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1; EC 3.1.4.11; Phosphoinositide phospholipase C-delta-1; Phospholipase C-III; PLC-III; Phospholipase C-delta-1; PLC-delta-1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-756
  • 氨基酸序列
    MDSGRDFLTL HGLQDDEDLQ ALLKGSQLLK VKSSSWRRER FYKLQEDCKT IWQESRKVMR TPESQLFSIE DIQEVRMGHR TEGLEKFARD VPEDRCFSIV FKDQRNTLDL IAPSPADAQH WVLGLHKIIH HSGSMDQRQK LQHWIHSCLR KADKNKDNKM SFKELQNFLK ELNIQVDDSY ARKIFRECDH SQTDSLEDEE IEAFYKMLTQ RVEIDRTFAE AAGSGETLSV DQLVTFLQHQ QREEAAGPAL ALSLIERYEP SETAKAQRQM TKDGFLMYLL SADGSAFSLA HRRVYQDMGQ PLSHYLVSSS HNTYLLEDQL AGPSSTEAYI RALCKGCRCL ELDCWDGPNQ EPIIYHGYTF TSKILFCDVL RAIRDYAFKA SPYPVILSLE NHCTLEQQRV MARHLHAILG PMLLNRPLDG VTNSLPSPEQ LKGKILLKGK KLGGLLPPGG EGGPEATVVS DEDEAAEMED EAVRSRVQHK PKEDKLRLAQ ELSDMVIYCK SVHFGGFSSP GTPGQAFYEM ASFSENRALR LLQESGNGFV RHNVGHLSRI YPAGWRTDSS NYSPVEMWNG GCQIVALNFQ TPGPEMDVYQ GRFQDNGACG YVLKPAFLRD PNGTFNPRAL AQGPWWARKR LNIRVISGQQ LPKVNKNKNS IVDPKVTVEI HGVSRDVASR QTAVITNNGF NPWWDTEFAF EVVVPDLALI RFLVEDYDAS SKNDFIGQST IPLNSLKQGY RHVHLMSKNG DQHPSATLFV KISLQD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development. Binds phosphatidylinositol 4,5-bisphosphate.
  • 基因功能参考文献：
    1. A loss of epidermal PLCdelta1 impairs epidermal barrier function through dysregulation of Ca2+ and p38 mitogen-activated protein kinase (MAPK) signaling. This study also reveals a possible link among PLCdelta1 downregulation, p38 MAPK hyperactivation, and barrier defects in psoriasis-like skin inflammation. PMID: 28430185
    2. PLCD1 acts as a tumour suppressor, by KIF3A-mediated suppression of ERK1/2/beta-catenin/MMP7 signalling, at least in part, in breast cancer. PMID: 28423710
    3. Nine of the 32 (28.1%) iCCA patients had gene mutations at chromosome 3p, totaling 11 mutations across five genes. Those included five (15.6%) BAP1 mutations, two each (6.3%) of CACNA2D3 and RASSF1 mutations, and one each (3.1%) of ATG7 and PLCD1 mutations. Six (18.8%) cases had concurrent loss of chromosome 3p and gene mutations. PMID: 29122566
    4. The PLCdelta1 negatively regulates autophagy, and PLCdelta1 suppression contributes to the tolerance of CRC cells harboring KRAS mutations to nutrient deprivation and anti-cancer drug treatment. PMID: 28528980
    5. Phospholipase C delta 1 is both a KLF15-regulated gene and a novel repressor of airway smooth muscle hypertrophy. PMID: 28375666
    6. High PLC delta expression is associated with breast cancer. PMID: 28112359
    7. slow phase of Gi/o-mediated TRPC4 activation was diminished by inhibiting RhoA or enhancing PLCdelta function PMID: 26755577
    8. Both unsaturated and saturated dioleoyl-phosphatidylinositol-(4,5)-bisphosphate successfully recruited PHPLCdelta1 to cell membranes. PMID: 25995263
    9. Neuropeptide Y decreased PLCD1 expression in HUVECs. PMID: 24903829
    10. PLC-delta1 and TRPV6 are critical actor of Ca(2+) homeostasis in CF human bronchial epithelial cells. PMID: 25477137
    11. Ectopic expression of PLCD1 inhibits breast tumor cell proliferation in vivo by inducing apoptosis and suppressed tumor cell migration by regulating cytoskeletal reorganization. PMID: 25655282
    12. PLCdelta1 is required for maintenance of homeostasis in skin and metabolic tissues. [review] PMID: 23948486
    13. Provide evidence for intramolecular interactions in the PLC-delta1 PH domain. PMID: 23388389
    14. Genotyping using microsatellite markers linked the families to the gene PLCD1 on chromosome 3p22.2. Sequence analysis of the gene detected one novel (p.Ser740ArgfsX19) and one previously reported mutation (p.Arg437X). PMID: 23149345
    15. A novel mutation in the PLCD1 gene, which leads to an aberrant splicing event, underlies autosomal recessive leuconychia. PMID: 22458588
    16. PLCD1 acts as a functional tumor suppressor and may serve as a biomarker for possible early detection and prognosis of chronic myeloid leukemia. PMID: 22576628
    17. Increased PLC-delta1 activity causes enhanced coronary vasomotility such as that seen in patients with coronary spastic angina. PMID: 22265909
    18. Mutations in this gene cause hereditary porcelain nails. Review. PMID: 22078044
    19. Significantly deregulated pathways in colorectal cancer were identified and repression of PLCD1 and PLCE1 expression, was validated. PMID: 21909432
    20. these data show that mutations in PLCD1 underlie hereditary leukonychia, revealing a gene involved in molecular control of nail growth. PMID: 21665001
    21. PLCD1 is silenced by promoter methylation in breast cancer. Silencing of PLCD1 is reversed by demethylation which leads to the disruption of cell cycle at G2/M phase in breast cancer, indicating that PLCD1 functions as a tumor suppressor in this tumor. PMID: 20657189
    22. After PLC-delta 1 treatment with small interfering RNA, angiotensin II type 1 receptor-stimulated inositol phosphate formation is attenuated. PMID: 20018811
    23. Mechanism of 17-beta-estradiol-induced Erk1/2 activation in breast cancer cells. A role for HER2 AND PKC-delta PMID: 11960991
    24. the PLC-delta1 promoter is under the control of NF-kappaB, which mediates the expression of PLC-delta due to Abeta42 PMID: 14550290
    25. PLCdelta(1) and PLCdelta(4) are probably differentially regulated in distinct cellular compartments by PI(4,5)P(2) PMID: 15037625
    26. Nuclear translocation of phospholipase C-delta1 is linked to the cell cycle and nuclear phosphatidylinositol 4,5-bisphosphate PMID: 15809301
    27. the Ral-CaM complex defines a multifaceted regulatory mechanism for PLC-delta1 activation PMID: 15817490
    28. In this review, recent findings regarding the molecular machineries and mechanisms of the nucleocytoplasmic shuttling of PLCdelta1 will be discussed. PMID: 16240320
    29. These results support the model that activation of selected PLC isoforms at the cleavage furrow controls progression of cytokinesis through regulation of PIP2 levels PMID: 17041247
    30. PLC delta 1 plays an important suppressive role in the development and progression of esophageal squamous cell carcinoma PMID: 18006814
    31. Human ARHGAP6 protein possessing GTPase stimulating activity for RhoA on the catalytic properties of PLC-delta1, was studied. PMID: 18434237
    32. Data are consistent with the idea that phospholipase C d1 and d3 isoforms support the growth and migration of normal and neoplastic mammary epithelial cells in vitro. PMID: 19344632
    33. PLCD1 acts as a functional tumor suppressor involved in gastric carcinogenesis. PMID: 19448674

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  • 相关疾病：
    Nail disorder, non-syndromic congenital, 3 (NDNC3)
  • 组织特异性：
    Strongly expressed in lung, liver and heart. Also expressed at least in pancreas, kidney, skeletal muscle, placenta and brain.
  • 数据库链接：

    HGNC: 9060

    OMIM: 151600

    KEGG: hsa:5333

    STRING: 9606.ENSP00000430344

    UniGene: Hs.80776