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  4. Recombinant High-energy light unresponsive protein 1 (lite-1), partial

Recombinant High-energy light unresponsive protein 1 (lite-1), partial

  • 中文名称：
    秀丽隐杆线虫lite-1重组蛋白
  • 货号：
    CSB-YP623148CXY1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    秀丽隐杆线虫lite-1重组蛋白
  • 货号：
    CSB-EP623148CXY1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    秀丽隐杆线虫lite-1重组蛋白
  • 货号：
    CSB-EP623148CXY1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    秀丽隐杆线虫lite-1重组蛋白
  • 货号：
    CSB-BP623148CXY1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    秀丽隐杆线虫lite-1重组蛋白
  • 货号：
    CSB-MP623148CXY1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    lite-1
  • Uniprot No.：
  • 别名：
    lite-1; gur-2; C14F11.3; High-energy light unresponsive protein 1
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Photoreceptor for short wavelength (UV) light that mediates UV-light-induced avoidance behavior. Directly senses and absorbs both UV-A and UV-B light with very high efficiency. Absorption of UV-B but not UV-A light shows resistance to photobleaching. In contrast to other photoreceptors, does not use a prosthetic chromophore to capture photons and only depends on its protein conformation. Might have a role in response to white light exposure.
  • 基因功能参考文献：
    1. Unlike typical photoreceptors employing a prosthetic chromophore to capture photons, LITE-1 strictly depends on its protein conformation for photon absorption; further two tryptophan residues critical for LITE-1 function were identified. Interestingly, unlike GPCRs, LITE-1 adopts a reversed membrane topology. Thus, LITE-1, a taste receptor homolog, represents a distinct type of photoreceptor in the animal kingdom. PMID: 27863243
    2. receptor family members LITE-1 and GUR-3 are required for the inhibition of feeding by light and hydrogen peroxide. PMID: 25640076
    3. data suggest a model in which LITE-1 transduces light signals in a photoreceptor neuron via G protein signaling, leading to upregulation of the second messenger cGMP, followed by opening of cGMP-sensitive CNG channels and stimulation of photoreceptors PMID: 20436480
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Insect chemoreceptor superfamily, Gustatory receptor (GR) family
  • 组织特异性：
    Expressed in the AVG and PVT neurons of the tail.
  • 数据库链接：

    KEGG: cel:CELE_C14F11.3

    STRING: 6239.C14F11.3

    UniGene: Cel.802