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  4. Recombinant Helicobacter pylori Urease subunit alpha (ureA)

Recombinant Helicobacter pylori Urease subunit alpha (ureA)

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  • 中文名称：
    幽门螺旋杆菌ureA重组蛋白
  • 货号：
    CSB-EP320452HUV
  • 规格：
    ￥2328
  • 促销：
    促销单品低至599元起
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    ureA
  • Uniprot No.：
  • 别名：
    ureA; hpuA; HP_0073; Urease subunit alpha; EC 3.5.1.5; Urea amidohydrolase subunit alpha
  • 种属：
    Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    30.5 kDa
  • 表达区域：
    1-238aa
  • 氨基酸序列
    MKLTPKELDKLMLHYAGELAKKRKEKGIKLNYVEAVALISAHIMEEARAGKKTAAELMQEGRTLLKPDDVMDGVASMIHEVGIEAMFPDGTKLVTVHTPIEANGKLVPGELFLKNEDITINEGKKAVSVKVKNVGDRPVQIGSHFHFFEVNRCLDFDREKTFGKRLDIASGTAVRFEPGEEKSVELIDIGGNRRIFGFNALVDRQADNESKKIALHRAKERGFHGAKSDDNYVKTIKE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The synthesis of Recombinant H.pylori ureA protein began with the isolation of ureA gene from the native genome. The next step was to amplify the ureA gene by inserting the ureA gene into an expression vector and then introducing the vector into the selected host cell, E.coli. Significant amounts of recombinant H.pylori ureA protein were produced by the host only when expression genes were added. For the purpose of protein purification, the vector contains N-terminal 6xHis tag to the ureA DNA sequence. The purity of this protein is 85%+ determined by SDS-PAGE.

    ureA (also known as hpuA) is a gene encoding a protein named urease subunit alpha (ureA) in Helicobacter pylori. The protein encoded by this gene is also known as urea amidohydrolase subunit alpha and belongs to urease gamma subunit family and urease beta subunit family. Its orthologous protein is known as the gamma/beta subunit (UreAB) in most other bacteria. This protein plays an important role in urea catabolic process.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach.
  • 亚细胞定位：
    Cytoplasm. Note=Also associates with the outer membrane upon autolysis of neighboring bacteria.
  • 蛋白家族：
    Urease gamma subunit family; Urease beta subunit family
  • 数据库链接：

    KEGG: heo:C694_00355

    STRING: 85962.HP0073