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  4. Recombinant Halobacterium salinarum Cobalamin-binding protein (btuF)

Recombinant Halobacterium salinarum Cobalamin-binding protein (btuF)

  • 中文名称：
    Recombinant Halobacterium salinarum Cobalamin-binding protein(btuF),Yeast
  • 货号：
    CSB-YP534746HTL
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Halobacterium salinarum Cobalamin-binding protein(btuF),Yeast
  • 货号：
    CSB-EP534746HTL-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Halobacterium salinarum Cobalamin-binding protein(btuF),Yeast
  • 货号：
    CSB-BP534746HTL
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Halobacterium salinarum Cobalamin-binding protein(btuF),Yeast
  • 货号：
    CSB-MP534746HTL
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    btuF
  • Uniprot No.：
  • 别名：
    btuF; OE_2951R; Cobalamin-binding protein; Vitamin B12-binding protein
  • 种属：
    Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-369
  • 氨基酸序列
    LAPQPP AQHADADRAC SFPVTEPDAS NTAITLDSEP ERVVTLNPSA AQTMWELGDR DAVVGVSQFG TYLPTASQRT VVSGGQPSQT NVEAVVGLDP DLVLAPNTVR NTTVTRLRSA GITVFQFRAA TSIDGVVEKT ATIGRLTGNC AAAAATTAEM RDRVAAIADA VPDTDSARPR VYYHLGDGYT AGPNTFIGAA IEAAGGHNIA ADVNTTSSYP QLSEEVIVSQ DPDVVVTGVS ADRLDATASA LVAPSSVVRN TTAYATGNVV AVNTNHINQP APRIVEPMAR MANAFHNTTI NTTLDAQPSA TTTATSTAPP TDAADGTAPG FGVAAAVCAL AGAALVARR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I had a couple of questions regarding your product CSB-YP534746HTL:
1.I plan to use this protein in AFM imaging experiments. Do you think aseptic processing and/or endotoxin removal are necessary for these experiments?
2.Can you advise whether or not this protein is suitable for lyophilization?
3.CSB-YP534746HTL (yeast), possible to have the tag removed?

A:
Very nice to receive your inquiry.
1.We think aseptic processing or endotoxin removal are not necessary for these experiments.
2.Glycerol will be added into the buffer for liquid protein. If the glycerol will affect your experiments, lyophilized protein is suitable.
3.CSB-YP534746HTL (yeast), possible to have the tag removed?
If you need to remove the tag of this protein, lead time will be extended for 3 business days. And we will charge for tag removal service, here it is the details about the Tag Removal Service:
Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge for extra cost.
If we fail in removing the tag, we won’t charge for tag removal and provide the fusion protein, and remark this information in datasheet as follows “Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”

靶点详情

  • 功能：
    Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin (vitamin B12) import. Probably binds cobalamin and delivers it to the surface of BtuC.
  • 数据库链接：

    KEGG: hsl:OE_2951R