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  4. Recombinant Griffithsia sp. Griffithsin (X31S)

Recombinant Griffithsia sp. Griffithsin (X31S)

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  • 中文名称：
    Recombinant Griffithsia sp. Griffithsin(X31S)
  • 货号：
    CSB-EP307563GDJ
  • 规格：
    ￥2328
  • 促销：
    促销单品低至599元起
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    N/A
  • Uniprot No.：
  • 种属：
    Griffithsia sp. (strain Q66D336) (Red alga)
  • 蛋白长度：
    Full length
  • 来源：
    E.coli
  • 分子量：
    20.2 kDa
  • 表达区域：
    1-121aa(X31S)
  • 氨基酸序列
    SLTHRKFGGSGGSPFSGLSSIAVRSGSYLDSIIIDGVHHGGSGGNLSPTFTFGSGEYISNMTIRSGDYIDNISFETNMGRRFGPYGGSGGSANTLSNVKVIQINGSAGDYLDSLDIYYEQY
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The production of this Recombinant Griffithsia sp. CHRNA1 protein began at the genetic level, where the coding sequence for the CHRNA1 protein was first isolated and cloned into an expression plasmid vector. Recombinant DNA technology was used in the process. Next step was cloning. The expression vector must be introduced into the host cell (E.coli) so that the cells could be cultured and expressed the desired Griffithsin protein. And we finally got the recombinant Griffithsin protein with the purity of 85%+ determined by SDS-PAGE.

    Griffithsin, derived from Griffithsia spp. marine red algae, is a small lectin consisting of 121 amino acids. Grifthsin is a domain-swapped dimer and each subunit has three nearly equivalent glycan-binding sites. Grifthsin binds glycan moieties associated with the glycoproteins of several enveloped viruses, resulting in inhibition of infectivity. Grifthsin has been shown to exhibit significant antiviral activity against human enveloped viruses including HIV, Middle East respiratory syndrome coronavirus (MERS-CoV), and severe acute respiratory syndrome corona virus (SARS-CoV), hepatitis C virus (HCV), herpes simplex virus 2 (HSV-2), and Japanese encephalitis virus (JEV). Moreover, Grifthsin shows excellent thermostability and is resistant to organic solvents and protease degradation. It possesses a superior safety profile: no cytotoxicity was observed against a variety of cell types, nor any major effects on peripheral blood mononuclear cell activation or cytokine and chemokine production. Thus, Grifthsin is an attractive candidate for development as an antiviral therapeutic.

     

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mixed specificity lectin with anti-HIV activity. Binds to HIV envelope glycoproteins, including exterior membrane glycoprotein gp120, and inhibits viral entry into cells. Binding to gp120 is dependent on gp120 being glycosylated, and is inhibited by mannose, glucose and N-acetylglucosamine.
  • 蛋白家族：
    Jacalin lectin family