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  4. Recombinant Eubacterium sp. Bile acid-CoA hydrolase (baiF)

Recombinant Eubacterium sp. Bile acid-CoA hydrolase (baiF)

  • 货号：
    CSB-YP321688EHF
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP321688EHF
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP321688EHF-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP321688EHF
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP321688EHF
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    baiF
  • Uniprot No.：
  • 别名：
    baiFBile acid-CoA transferase; EC 2.8.3.25; Bile acid-coenzyme A transferase; Bile acid-inducible operon protein F
  • 种属：
    Eubacterium sp. (strain VPI 12708)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-426
  • 氨基酸序列
    AGIKDFPKF GALAGLKILD SGSNIAGPLG GGLLAECGAT VIHFEGPKKP DNQRGWYGYP QNHRNQLSMV ADIKSEEGRK IFLDLIKWAD IWVESSKGGQ YDRLGLSDEV IWEVNPKIAI VHVSGYGQTG DPSYVTRASY DAVGQAFSGY MSLNGTTEAL KINPYLSDFV CGLTTCWAML ACYVSTILTG KGESVDVAQY EALARIMDGR MIQYATDGVK MPRTGNKDAQ AALFSFYTCK DGRTIFIGMT GAEVCKRGFP IIGLPVPGTG DPDFPEGFTG WMIYTPVGQR MEKAMEKYVS EHTMEEVEAE MQAHQIPCQR VYELEDCLND PHWKARGTIT EWDDPMMGHI TGLGLINKFK RNPSEIWRGA PLFGMDNRDI LKDLGYDDAK IDELYEQGIV NEFDLDTTIK RYRLDEVIPH MRKKEE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions in the bile acid 7alpha-dehydroxylation pathway, which forms secondary bile acids via the 7alpha-dehydroxylation of primary bile acids, and is carried out by intestinal anaerobic bacteria. Acts as a bile acid CoA transferase with broad bile acid substrate specificity. Catalyzes the transfer of the CoA moiety of secondary bile acid-CoA compounds to primary bile acids. Can use lithocholoyl-CoA, deoxycholoyl-CoA and allodeoxycholoyl-CoA as bile acid CoA donors and cholate, allocholate, chenodeoxycholate, ursodeoxycholate, and beta-muricholate as bile acid CoA acceptors. Also displays CoA hydrolase activity, being able to catalyze the hydrolysis of choloyl-CoA, 3-dehydrocholoyl-CoA, and chenodeoxycholoyl-CoA, releasing CoA and the corresponding free bile acid. However, this latter activity may not represent the actual activity of this enzyme, since using a transferase rather than hydrolase, the bacteria conserve the thioester bond energy, saving ATP molecules (Probable). Shows no hydrolytic activity with acetyl-CoA, isovaleryl-CoA, palmitoyl-CoA, or phenylacetyl-CoA as substrates.
  • 蛋白家族：
    CaiB/BaiF CoA-transferase family
  • 数据库链接：

    KEGG: ag:AAC45415