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  4. Recombinant Escherichia coli tRNA threonylcarbamoyladenosine biosynthesis protein TsaE (tsaE)

Recombinant Escherichia coli tRNA threonylcarbamoyladenosine biosynthesis protein TsaE (tsaE)

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  • 中文名称：
    大肠杆菌tsaE重组蛋白
  • 货号：
    CSB-EP360227ENVe1
  • 规格：
    ￥3324
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    tsaE
  • Uniprot No.：
  • 别名：
    tsaE; yjeE; b4168; JW4126tRNA threonylcarbamoyladenosine biosynthesis protein TsaE; t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    16.9kDa
  • 表达区域：
    1-153aa
  • 氨基酸序列
    MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTFSRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFANDAICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    Tag-Free
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro.
  • 基因功能参考文献：
    1. high-affinity binding of YjeE to ADP using equilibrium dialysis; YjeE hydrolyses ATP PMID: 15324301
    2. Repression of yjeE expression results in at least a proportion of very enlarged cells with an unusual peripheral distribution of DNA, showing that this gene is essential for the viability of E. coli. PMID: 19376873
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    TsaE family
  • 数据库链接：

    KEGG: ecj:JW4126

    STRING: 316385.ECDH10B_4363