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  4. Recombinant Escherichia coli UPF0350 protein ygfY (ygfY)

Recombinant Escherichia coli UPF0350 protein ygfY (ygfY)

  • 货号：
    CSB-YP354924ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP354924ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP354924ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP354924ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP354924ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    sdhE
  • Uniprot No.：
  • 别名：
    sdhE; cptB; ygfY; b2897; JW2865FAD assembly factor SdhE; Antitoxin CptB
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-88
  • 氨基酸序列
    MDINNKARIH WACRRGMREL DISIMPFFEH EYDSLSDDEK RIFIRLLECD DPDLFNWLMN HGKPADAELE MMVRLIQTRN RERGPVAI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    An FAD assembly protein, which accelerates covalent attachment of the cofactor into other proteins. Plays an essential role in the assembly of succinate dehydrogenase (SDH, respiratory complex II), an enzyme complex that is a component of both the tricarboxylic acid cycle and the electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit SdhA of SDH. Required for flavinylation of the flavoprotein subunit FdrA of fumarate reductase (FDR). Binds 2 different sites on the flavoprotein target FrdA (and presumably also SdhA), possibly positioning FAD and protein to facilitate the covalent bond formation; covalent attachment of FAD is not required for SDH or FDR complex enzyme formation. Overexpression of this protein and YgfX (formerly cptA) restores production of prodigiosin antibiotic (Pig) in Serratia strains with deletions of sdhE-ygfX.
  • 基因功能参考文献：
    1. These studies identify how the conserved SdhE assembly factor and its homologs participate in complex II maturation. PMID: 26644464
    2. SdhE interacted with, and flavinylated, the flavoprotein subunit FrdA, whilst mutations in a conserved RGxxE motif impaired the complete flavinylation and activation of FRD PMID: 24374335
    3. YgfY may be involved in transcriptional regulation, as shown by X-ray crystallographic analysis PMID: 15593094
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    SdhE FAD assembly factor family
  • 数据库链接：

    KEGG: ecj:JW2865

    STRING: 316385.ECDH10B_3071